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Bio-Analytical Liquid Chromatographic Method for the Determination of Linezolid in Plasma
Corresponding Author(s) : P.V.V. Satyanarayana
Asian Journal of Chemistry,
Vol. 25 No. 8 (2013): Vol 25 Issue 8
Abstract
An isocratic selective and sensitive high performance liquid chromatographic method for the estimation of linezolid in human plasma by precipitation of plasma proteins with 5 % perchloric acid, after precipitation the supernatant was separated and injecting 10 μL of sample volume. The chromatography separation was achieved on a Hypersil BDS C18 (4.6 ID × 250 mm, 5 μ particle size) column using a mixture of 20 Mm potassium dihydrogen phosphate buffer (pH 3.5 ± 0.1) and acetonitrile in a ratio of 85:15 (v/v) as the mobile phase. The column elutes were monitored at 252 nm. The total chromatographic run time was 12.5 min and the elution of linezolid and zidovudine (IS) retention times 10.5 and 5.2 respectively. The method was found to be linear in the concentration range of 50.0 to 20059.9 ng/mL. The method was successfully applied to in vitro pharmacokinetic-bioequivalence studies.
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- http://www.drugbank.ca/drugs/DB00601.
- http://www.rxlist.com/zyvox-drug.htm.
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- M.B. Lauren, L. Jian, L.N. Roger and R.R. Craig, Biomed. Chromatogr. BMC, 20, 782 (2006).
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- O. Sagirli, A. Onal, S. Toker and A. Oztunc, J. AOAC Int., 92, 1658 (2009).
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- D. Cattaneo, S.C.D. Baldelli, F.B.D. Conti, V.B.D. Cozzi, E.M.D. Clementi, Therap. Drug Monit., 32, 520 (2010).
- O.A. Phillips, M.E. Abdel-Hamid and N.A. Al-Hassawi, Analyst, 126, 609 (2001).
- V. Laurian, M. Dana, C. Magdalena, C. Rodica and G. Simion, J. Liq. Chromatogr. Rel. Technol., 34, 436 (2011).
- http://www/fda.gov/cder/guidance/index.htm
References
http://www.drugbank.ca/drugs/DB00601.
http://www.rxlist.com/zyvox-drug.htm.
S.A. Patel, P.U. Patel, N.J. Patel, M.M Patel and U.V. Bangoriya, Indian J. Pharm Sci., 69, 571 (2007).
J. Dharuman, V. Ravichandran, P.M. Krishna, S. Elsamul, S. Lavanya and C. Jayalakshmi, Indian J. Pharm. Sci., 66, 235 (2004).
M.B. Lauren, L. Jian, L.N. Roger and R.R. Craig, Biomed. Chromatogr. BMC, 20, 782 (2006).
B.B. Ba, B.B.B. Nso, C. Quentin and M.-C. Saux, J. Chromatogr. B., 854, 104 (2007).
O. Sagirli, A. Onal, S. Toker and A. Oztunc, J. AOAC Int., 92, 1658 (2009).
T. Friederike, M. Oliver, P. Martin and J. Christian, J. Chromatogr. Sci., 48, 325 (2010).
D. Cattaneo, S.C.D. Baldelli, F.B.D. Conti, V.B.D. Cozzi, E.M.D. Clementi, Therap. Drug Monit., 32, 520 (2010).
O.A. Phillips, M.E. Abdel-Hamid and N.A. Al-Hassawi, Analyst, 126, 609 (2001).
V. Laurian, M. Dana, C. Magdalena, C. Rodica and G. Simion, J. Liq. Chromatogr. Rel. Technol., 34, 436 (2011).
http://www/fda.gov/cder/guidance/index.htm