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Vol. 25 No. 8 (2013): Vol 25 Issue 8

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Electrochemical DNA Biosensor Improved by Imidazo[4,5-f]1,10-phenanthroline Iron(III) as an Indicator based on Pt-nanoparticles and Carbon Nanotubes Modified Electrode

https://doi.org/10.14233/ajchem.2013.13983
Shu-Yan Niu
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China
Ying-Qiang Qin
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China
Bin Han
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China

Corresponding Author(s) : Shu-Yan Niu

wenzhangggg@126.com

Asian Journal of Chemistry, Vol. 25 No. 8 (2013): Vol 25 Issue 8

Abstract

A novel and sensitive electrochemical DNA biosensor has been developed for the detection of DNA hybridization. The biosensor was proposed by using imidazo[4,5-f]1,10-phenanthroline iron(III) (Fe(phen)2IP·3ClO4·2H2O, abbreviated as [Fe(phen)2IP]3+), as an electroactive indicator based on multi-walled carbon nanotubes/platinum nanoparticles (MWCNTs/Ptnano) modified glassy carbon electrode (GCE). The interaction between [Fe(phen)2IP]3+ and double-stranded DNA (dsDNA) was investigated by cyclic voltammetry (CV). The experiment results showed that [Fe(phen)2IP]3+ presented an excellent electrochemical activity on GCE and could intercalate into the double helix of double-stranded DNA. The target ssDNA of the human hepatitis B virus (HBV) was quantified in a linear range from 1.13 × 10-11-1.13 × 10-9 M (r = 0.9892) with a detection limit of 5.56 × 10-12 M (3s, n = 11).

Keywords

Fe(phen)2IP·3ClO4·2H2O Carbon nanotubes Pt-nanoparticles DNA Hybridization Electrochemical DNA biosensor
Niu, S.-Y., Qin, Y.-Q., & Han, B. (2013). Electrochemical DNA Biosensor Improved by Imidazo[4,5-f]1,10-phenanthroline Iron(III) as an Indicator based on Pt-nanoparticles and Carbon Nanotubes Modified Electrode. Asian Journal of Chemistry, 25(8), 4375–4379. https://doi.org/10.14233/ajchem.2013.13983
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