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Vol. 25 No. 8 (2013): Vol 25 Issue 8

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Determination of Gossypol in Poultry Egg and Feed by Automated Solid-Phase Extraction and High-Performance Liquid Chromatography

https://doi.org/10.14233/ajchem.2013.14061
Zu-Hao Shi
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Meng-Jun Tang
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Xiao-Yan Zhang
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Zhi-Yun Tao
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Ming Wu
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Yu-Shi Gao
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Rong Gu
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Jun-Xian Lu
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Jun-hua Pu
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China
Qing-Lian Ge
Quality Inspection Center for Poultry of Ministry of Agriculture, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, P.R. China

Corresponding Author(s) : Yu-Shi Gao

vetszh@gmail.com

Asian Journal of Chemistry, Vol. 25 No. 8 (2013): Vol 25 Issue 8

Abstract

A high-performance liquid chromatographic method for the determination of gossypol in poultry egg and feed was developed. Gossypol was extracted with 90 % acetonitrile/water (v/v) in presence of ascorbic acid and purified by C18 cartridge with automated solid-phase extraction system. The samples were separated through an optimized chromatography condition on a C18 column (4.6 mm × 250 mm, 5 μm particle) and UV detection at 235 nm. A gradient with acetonitrile and water containing 0.1 % phosphoric acid was applied. The method was shown to be highly reproducible, with overall recovery was 89.5 % and relative standard deviation was 8.7 %.

Keywords

Gossypol High-performance liquid chromatography Egg Poultry feed
Shi, Z.-H., Tang, M.-J., Zhang, X.-Y., Tao, Z.-Y., Wu, M., Gao, Y.-S., … Ge, Q.-L. (2013). Determination of Gossypol in Poultry Egg and Feed by Automated Solid-Phase Extraction and High-Performance Liquid Chromatography. Asian Journal of Chemistry, 25(8), 4547–4550. https://doi.org/10.14233/ajchem.2013.14061
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References
  1. J. Colin-Negrete, H.E. Kiesling, T.T. Ross and J.F. Smith, J. Dairy Sci., 79, 2016 (1996).
  2. S.Z. Qian and Z.G. Wang, Ann. Rev. Pharmacol. Toxicol., 24, 329 (1984).
  3. R.L. Herman, J. Fish Biol., 2, 293 (1970).
  4. J. Rinchard, A. Ciereszko, K. Dabrowski and J. Ottobre, Toxicol. Lett., 111, 189 (2000).
  5. K. Dabrowski, J. Rinchard, K.-J. Lee, J.H. Blom, A. Ciereszko and J. Ottobre, Biol. Reprod., 62, 227 (2000).
  6. C. Van Poznak,A.D Seidman, M.M. Reidenberg, M.M. Moasser, N. Sklarin, K. Van Zee, P. Borgen, M. Gollub, D. Bacotti, T.J. Yao, R. Bloch, M. Ligueros, M. Sonenberg, L. Norton and C. Hudis, Breast Cancer Res Treat., 66, 239 (2001).
  7. M.J. Sikora, J.A. Bauer, M. Verhaegen, T.J. Belbin, M.B. Prystowsky, J.C. Taylor, J.C. Brenner, S. Wang, M.S. Soengas, C.R. Bradford and T.E. Carey, Cancer Biol. Ther., 7, 767 (2008).
  8. M.D. Shelley, L. Hartley, P.W. Groundwater and R.G. Fish, Anticancer Drugs., 11, 209 (2000).
  9. G.P. Tuszynski and G. Cossu, Cancer Res., 44, 768 (1984).
  10. A.S. El-Sharaky, M.M. Wahby, M.M. Bader El-Dein, R.A. Fawzy and I.N. El-Shahawy, Food Chem. Toxicol., 47, 2735 (2009).
  11. P.M. Zavos and P.N. Zarmakoupis-Zavos, Tohoku J. Exp. Med., 179, 167 (1996).
  12. K. Dabrowski, K.J. Lee, J. Rinchard, A. Ciereszko, J.H. Blom and J.S. Ottobre, Biochim. Biophys. Acta, 1525, 37 (2001).
  13. M. Nakamura, M. Ikeda, M. Komukai, T. Suyemitsu, S. Okinaga and K. Arai, Hum Reprod., 6, 714 (1991).
  14. B. Polsky, S.J. Segal, P.A. Baron, J.W. Gold, H. Ueno and D. Armstrong, 39, 579 (1989).
  15. K. Ratsula, M. Haukkamaa, K. Wichmann and T. Luukkainen, Contraception, 27, 571 (1983).
  16. L. Qin, X.J. Shen, J.H. Chen and S.J. Zhu, Guang Pu Xue Yu Guang Pu Fen Xi, 30, 635 (2010).
  17. N.A. Botsoglou and D.C. Kufidis, J. Assoc. Off. Anal. Chem., 73, 447 (1990).
  18. M.A. McClure, J. Chromatogr. A, 54, 25 (1971).
  19. G.B. Marcelle, M.S. Ahmed, J.M. Pezzuto, G.A. Cordell, D.P. Waller, D.D. Soejarto and H.H. Fong, J. Pharm. Sci., 73, 396 (1984).
  20. K.J. Lee, K. Dabrowski, J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 779, 313 (2002).
  21. X. Cui, C. Xie, L. Zhang and Z. Jin, Se Pu, 22, 559 (2004).
  22. R. Meyer, S. Vorster and I.A. Dubery, Anal. Bioanal. Chem., 380, 719 (2004).
  23. N.A. Botsoglou, J. Chromatogr. A, 587, 333 (1991).
  24. H.L. Kim, M.C. Calhoun and R.D. Stipanovic, Comp. Biochem. Physiol. B: Biochem. Mol. Biol., 113, 417 (1996).
  25. R.B. Fombad and M.J. Bryant, Trop. Anim. Health Prod., 36, 295 (2004).
  26. K.J. Lee, K. Dabrowski, J.H. Blom, S.C. Bai and P.C. Stromberg, J. Anim. Physiol. Anim. Nutr. (Berl.), 86, 201 (2002).
  27. K.J. Lee and K. Dabrowski, J. Agric. Food. Chem., 50, 3056 (2002).
  28. A. Tse-Yan Lee, Volume 2 Number 1 (2005); http://curezone.com/forums/fm.asp?i=863091 (2005).
  29. M.-Z. Wang, D.-F. Wu and Y.-W. Yu, J. Chromatogr. A, 343, 387 (1985).
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