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Vol. 25 No. 7 (2013): Vol 25 Issue 7

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Pressurized Liquid Extraction and HPLC Analysis for Determination of Polyphenols in Tobacco

https://doi.org/10.14233/ajchem.2013.13832
Zhiyan Chen
Technical Center, China Tobacco Guangxi Industrial Corporation, Nanning 530001, P.R. China
Shijie Huang
Technical Center, China Tobacco Guangxi Industrial Corporation, Nanning 530001, P.R. China
Qingde Su
Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China
Xiaolan Zhu
Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China

Corresponding Author(s) : Xiaolan Zhu

zxl8906@ustc.edu.cn

Asian Journal of Chemistry, Vol. 25 No. 7 (2013): Vol 25 Issue 7

Abstract

A simple and efficient pressurized liquid extraction followed by HPLC analysis method has been investigated for the extraction of polyphenols in tobacco. After pressurized liquid extraction and C18 disposable cartridge solid-phase extraction clean-up, to reduce the co-extraction of interference, polyphenols (chlorogenic acid, esculetin, rutin, scopoletin and quercitrin) were analyzed by HPLC with a diode array detector. When the method was validated, recoveries obtained were varied from 86.9 to 96.4 % and RSDs from 3.4 to 8.2 %.

Keywords

Pressurized liquid extraction Polyphenol Tobacco Solid-phase extraction
Chen, Z., Huang, S., Su, Q., & Zhu, X. (2013). Pressurized Liquid Extraction and HPLC Analysis for Determination of Polyphenols in Tobacco. Asian Journal of Chemistry, 25(7), 3889–3892. https://doi.org/10.14233/ajchem.2013.13832
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