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Vol. 25 No. 7 (2013): Vol 25 Issue 7

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Structural Identification of Chemical Constituents from Scutellaria baicalensis by HPLC-ESI-MS/MS and NMR Spectroscopy

https://doi.org/10.14233/ajchem.2013.13788
Wenhui Lin
School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, P.R. China
Shuang Liu
Haerbin University of Commerce, Haerbin 150076, P.R. China ; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd, Zhangjiang, Pudong 201203, Shanghai, P.R. China
Bin Wu
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd, Zhangjiang, Pudong 201203, Shanghai, P.R. China

Corresponding Author(s) : Bin Wu

wubin1031@hotmail.com; 893898379@qq.com; linwu1217@hotmail.com

Asian Journal of Chemistry, Vol. 25 No. 7 (2013): Vol 25 Issue 7

Abstract

Scutellariae Radix, the dried roots of Scutellaria baicalensis Georgi, which is one of the famous herb widely used in the treatment of pyrexia, jaundice and hypertension. To directly identify the compounds of the extract of the S. baicalensis was performed using HPLC-ESI-MS/MS. Chromatographic (analytical and preparative HPLC), chemical (hydrolysis) and spectroscopic (UV, 1H and 13C NMR, MS) techniques were applied for separation, isolation, purification and identification of new compounds. A systematic phytochemical investigantion of S. baicalensis led to the isolation of 7 new and 40 known chemical constituents. By HPLC-ESI-MS/MS and NMR spectral analyses, the new compounds were identified as 7-hydroxy-3,5,8-trimethoxy-3',4'-methylenedioxy flavone-7-O-b-glucopyranoside (1), 7-hydroxy-5,6,8-trimethoxy-3',4'-methylenedioxy flavone-7-O-b-glucopyranoside (2), patuletin-7-b-glucuronide (3), pinobankasin-6-C-glucopyranosyl-8-C-arabinopyranoside (4), chrysin-3-C-a-arabinopyranosyl-8-C-b-glucopyranoside (11), 6-sulfooxy-chrysin-7-glucoside (12) and 5-hydoxy-6,7,4'-trimethoxy-flavanone 5-sulfate (24).

Keywords

Scutellaria baicalensis; Structural elucidation Chemical constituents HPLC-MS/MS NMR
Lin, W., Liu, S., & Wu, B. (2013). Structural Identification of Chemical Constituents from Scutellaria baicalensis by HPLC-ESI-MS/MS and NMR Spectroscopy. Asian Journal of Chemistry, 25(7), 3799–3805. https://doi.org/10.14233/ajchem.2013.13788
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