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Antioxidant Activity of Consecutive Extracts of the Base, Stem and Leaves of Etlingera brevilabrum
Corresponding Author(s) : B. Mahdavi
Asian Journal of Chemistry,
Vol. 25 No. 7 (2013): Vol 25 Issue 7
Abstract
The base, stem and leaves of Etlingera brevilabrum were extracted consecutively with hexane, ethyl acetate, acetone, methanol, methanol:water (1:1) and water and also solely with ethanol. The total phenolic content and antioxidant assays of DPPH radical scavenging activity, b-carotene bleaching, ferrous ion chelating ability and reducing power were carried out on the extracts. In most of the assays, the leaf extracts showed more activity than the others. The methanolic extract of leaf showed the highest total phenolic content and inhibition of b-carotene bleaching. The methanol:water extract of the leaf exhibited maximum DPPH radical scavenging and reducing power activity. However, the base methanolic extract showed the highest ferrous ion chelating ability, even higher than the positive controls of ascorbic acid and citric acid. All the extracts gave good linear correlations between total phenolic content and radical scavenging activity as well as between total phenolic content and reducing power.
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- M. Habsah, M. Amran, M.M. Mackeen, N.H. Lajis, H. Kikuzaki, N. Nakatani, A.A. Rahman, Ghafar and A.M. Ali, J. Ethnopharmacol., 72, 403 (2000).
- S.H. Khaw, Gardens' Bull. Singapore, 53, 191239 (2001).
- E.W.C. Chan, Y.Y. Lim, S.K. Ling, S.P. Tan, K.K. Lim and M.G.H. Khoo, LWT-Food Sci. Technol., 42, 1026 (2009).
- E.W.C. Chan, Y.Y. Lim and M. Omar, Food Chem., 104, 1586 (2007).
- A.D. Poulsen, Etlingera of Borneo, Natural History Publications (Borneo) in Association with Royal Botanic Garden Edinburgh, edn. 1, p. 263 (2006).
- E.W.C. Chan, Y.Y. Lim, L.F. Wong, F.S. Lianto, S.K. Wong, K.K. Lim, C.E. Joe and T.Y. Lim, Food Chem., 109, 477 (2008).
- A.A. Elzaawely, T.D. Xuan and S.H. Koyamaand, Food Chem., 104, 1648 (2007).
- A.H. El-Ghorab, M. Nauman, F.M. Anjum, S. Hussain and M. Nadeem, J. Agric. Food Chem., 58, 8231 (2010).
- X. Liu, S. Ardo, M. Bunning, J. Parry, K. Zhou, C. Stushnoff, F. Stoniker, L. Yu and P. Kendall, LWT-Food Sci. Technol., 40, 552 (2007).
- F. Xu, L. Li, X. Huang, H. Cheng, Y. Wang and H. Cheng, J. Med. Plants Res., 4, 2544 (2010).
- G.C. Yen and H.Y. Chen, J. Agric. Food Chem., 43, 27 (1995).
- A.A. Karagözler, E. Bengi, Y.E. Çalmaz and D.A.Uygun, Food Chem., 111, 400 (2008).
- A. von Gadow, E. Joubert and C.F. Hansmann, J. Agric. Food Chem., 45, 632 (1997).
- X.J. Duan, W.W. Zhang, X.M. Li and B.G. Wang, Food Chem., 95, 37 (2006).
- N. Singh and P.S. Rajini, Food Chem., 85, 611 (2004).
- M.H. Alma,A. Mavi,A. Yildirim, M. Digrak and T. Hirata, Biol. Pharm. Bull., 26, 1725 (2003).
- H. Qi, X. Liu, J. Ma, Q, Zhang and Z. Li, J. Med. Plants Res., 4, 2445 (2010).
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- A. Ismail, Z.M. Marjan and C.W. Foong, Food Chem., 87, 581 (2004).
- Y.S. Velioglu, G. Mazza, L. Gao and B.D. Oomah, J. Agric. Food Chem., 46, 4113 (1998).
- G.C. Yen, P.D. Duh and C.L. Tsai, J. Agric. Food Chem., 41, 67 (1993).
- T.Y. Lim, Y.Y. Lim and C.M. Yule, Food Chem., 114, 594 (2009).
- N. Andarwulan, R. Batari, D.A. Sandrasari, B. Bolling and H. Wijaya, Food Chem., 121, 1231 (2010).
- M. Roche, C. Dufour, N. Mora and O. Dangles, Org. Biomol. Chem., 3, 423 (2005).
- B. Tepe, O. Eminagaoglu, H.A. Akpulat and E. Aydin, Food Chem., 100, 985 (2007).
- D. Villaño, M.S. Fernández-Pachón, M.L. Moyá and A.M. Troncoso, Talanta, 71, 230 (2007).
- J.K. Moon and T. Shibamoto, J. Agric. Food Chem., 57, 1655 (2009).
- T. Kulisic, A. Radonic, V. Katalinic and M. Milos, Food Chem., 85, 633 (2004).
- M. Antolovich, P.D. Prenzler, E. Patsalides, S. McDonald and K. Robards, Analyst, 127, 183 (2002).
- L.L. Stookey, Anal. Chem., 42, 779 (1970).
- Ì. Gülçin, I.G. Sat, S. Beydemir, M. Elmastas and Ö.I. Küfrevioglu, Food Chem., 87, 393 (2004).
- T. Wang, R. Jónsdóttir and G. Ólafsdóttir, Food Chem., 116, 240 (2009).
- N. Mohd-Esa, F.S Hern, A. Ismail and C.L. Yee, Food Chem., 122, 1055 (2010).
- M. Hassas-Roudsari, P.R. Chang, R.B. Pegg and R.T. Tyler, Food Chem., 114, 717 (2009).
References
M. Habsah, M. Amran, M.M. Mackeen, N.H. Lajis, H. Kikuzaki, N. Nakatani, A.A. Rahman, Ghafar and A.M. Ali, J. Ethnopharmacol., 72, 403 (2000).
S.H. Khaw, Gardens' Bull. Singapore, 53, 191239 (2001).
E.W.C. Chan, Y.Y. Lim, S.K. Ling, S.P. Tan, K.K. Lim and M.G.H. Khoo, LWT-Food Sci. Technol., 42, 1026 (2009).
E.W.C. Chan, Y.Y. Lim and M. Omar, Food Chem., 104, 1586 (2007).
A.D. Poulsen, Etlingera of Borneo, Natural History Publications (Borneo) in Association with Royal Botanic Garden Edinburgh, edn. 1, p. 263 (2006).
E.W.C. Chan, Y.Y. Lim, L.F. Wong, F.S. Lianto, S.K. Wong, K.K. Lim, C.E. Joe and T.Y. Lim, Food Chem., 109, 477 (2008).
A.A. Elzaawely, T.D. Xuan and S.H. Koyamaand, Food Chem., 104, 1648 (2007).
A.H. El-Ghorab, M. Nauman, F.M. Anjum, S. Hussain and M. Nadeem, J. Agric. Food Chem., 58, 8231 (2010).
X. Liu, S. Ardo, M. Bunning, J. Parry, K. Zhou, C. Stushnoff, F. Stoniker, L. Yu and P. Kendall, LWT-Food Sci. Technol., 40, 552 (2007).
F. Xu, L. Li, X. Huang, H. Cheng, Y. Wang and H. Cheng, J. Med. Plants Res., 4, 2544 (2010).
G.C. Yen and H.Y. Chen, J. Agric. Food Chem., 43, 27 (1995).
A.A. Karagözler, E. Bengi, Y.E. Çalmaz and D.A.Uygun, Food Chem., 111, 400 (2008).
A. von Gadow, E. Joubert and C.F. Hansmann, J. Agric. Food Chem., 45, 632 (1997).
X.J. Duan, W.W. Zhang, X.M. Li and B.G. Wang, Food Chem., 95, 37 (2006).
N. Singh and P.S. Rajini, Food Chem., 85, 611 (2004).
M.H. Alma,A. Mavi,A. Yildirim, M. Digrak and T. Hirata, Biol. Pharm. Bull., 26, 1725 (2003).
H. Qi, X. Liu, J. Ma, Q, Zhang and Z. Li, J. Med. Plants Res., 4, 2445 (2010).
M. Moyo, A.R. Ndhlala, J.F. Finnie and J. Van Staden, Food Chem., 123, 69 (2010).
S. Serçe, M. Özgen, A.A. Torun and S. Ercisli, J. Food Comp. Anal., 23, 619 (2010).
A. Ismail, Z.M. Marjan and C.W. Foong, Food Chem., 87, 581 (2004).
Y.S. Velioglu, G. Mazza, L. Gao and B.D. Oomah, J. Agric. Food Chem., 46, 4113 (1998).
G.C. Yen, P.D. Duh and C.L. Tsai, J. Agric. Food Chem., 41, 67 (1993).
T.Y. Lim, Y.Y. Lim and C.M. Yule, Food Chem., 114, 594 (2009).
N. Andarwulan, R. Batari, D.A. Sandrasari, B. Bolling and H. Wijaya, Food Chem., 121, 1231 (2010).
M. Roche, C. Dufour, N. Mora and O. Dangles, Org. Biomol. Chem., 3, 423 (2005).
B. Tepe, O. Eminagaoglu, H.A. Akpulat and E. Aydin, Food Chem., 100, 985 (2007).
D. Villaño, M.S. Fernández-Pachón, M.L. Moyá and A.M. Troncoso, Talanta, 71, 230 (2007).
J.K. Moon and T. Shibamoto, J. Agric. Food Chem., 57, 1655 (2009).
T. Kulisic, A. Radonic, V. Katalinic and M. Milos, Food Chem., 85, 633 (2004).
M. Antolovich, P.D. Prenzler, E. Patsalides, S. McDonald and K. Robards, Analyst, 127, 183 (2002).
L.L. Stookey, Anal. Chem., 42, 779 (1970).
Ì. Gülçin, I.G. Sat, S. Beydemir, M. Elmastas and Ö.I. Küfrevioglu, Food Chem., 87, 393 (2004).
T. Wang, R. Jónsdóttir and G. Ólafsdóttir, Food Chem., 116, 240 (2009).
N. Mohd-Esa, F.S Hern, A. Ismail and C.L. Yee, Food Chem., 122, 1055 (2010).
M. Hassas-Roudsari, P.R. Chang, R.B. Pegg and R.T. Tyler, Food Chem., 114, 717 (2009).