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Determination of Geniposide in Rat Plasma by UPLC-MS
Corresponding Author(s) : Tong Zhang
Asian Journal of Chemistry,
Vol. 25 No. 7 (2013): Vol 25 Issue 7
Abstract
A sensitive ultra performance liquid chromatography-mass spectrometry method has been developed and validated for the quantification of geniposide in rat plasma. Following solid-phase extraction, the analytes were separated on a UPLC BEH C18 column and analyzed in the selected ion recording with a positive ionization mode. The method was linear over the concentration range of 16.875-2700 ng/mL. Intra-day and inter-day precisions and accuracy were all within 15 % range. The lower limit of quantification was 16.875 ng/mL. The recovery of geniposide was more than 65 %. The present method was successfully applied for the estimation of the pharmacokinetic parameters of geniposide following oral administration to rats at different three doses.
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- Chinese Pharmacopoeia Committee Editing. Chinese Pharmacopoeia, Chemical and Industrial Publisher, Part I, 2005 edition, p. 173 (2005).
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Z.J. Zhu, Z.Y. Qiao and C.Y. Bao, Chin. Tradit. Herb. Drugs, 32, 530 (2001).
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H.Y. Hsu, E.J. Yang, S.Y. Lin and C.C. Lin, Cancer Lett., 113, 31 (1997).
Y. Suzuki, K. Kondo, Y. Ikeda and K. Umemura, Planta Med., 67, 807 (2001).
H.J. Koo, K.H. Lim, H.J. Jung and E.H. Park, J. Ethnopharmacol., 103, 496 (2006).
M.J. Lee, J.D. Hsu and C.J. Wang, Anti Cancer Res., 15, 411 (1995).
Q.C. Chen, W.Y. Zhang, H. Kim, I.S. Lee, Y. Ding, U.J. Youn, S.M. Lee, M. Na, B.S. Min and K. Bae, Phytother. Res., 24S, S1 (2010).
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G. Ye, H.Y. Zhu, H.L. Zhao, B. Xu and C.G. Huang, Biomed. Chromatogr., 20, 743 (2006).
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