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Spectrophotometric Study of the Biocoordination Chemistry of Strontium Nitrate and Glutathione
Corresponding Author(s) : A. Rasul
Asian Journal of Chemistry,
Vol. 25 No. 7 (2013): Vol 25 Issue 7
Abstract
The effect of strontium nitrate on the status of glutathione in aqueous media was examined spectrophotometrically. The wavelength of 412 nm was used for the determination of thiol concentration in samples in accordance with a well known Elman's method. During the experiments the pH was maintained at 7.6 by phosphate buffer which was proposed to be suitable for performing in vitro experiments on thiols by Evans. When glutathione was exposed to different concentrations of strontium nitrate in aqueous solution, there was a significant decrease in the concentration of glutathione which means that strontium nitrate causes chemical modulation of glutathione and converts this molecule to its oxidized or disulfide form or glutathione-strontium complex. Time dependent, temperature and pH effect of strontium nitrate on the chemical status of glutathione was also examined. It was observed that there was a decrease in concentration of glutathione with these parameters. This study was carried out in in vitro condition to establish the model of in vivo reaction. The exact mechanism of reaction between metal and glutathione still need to be investigated but formation of strontium-glutathione complex may be proposed.
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- B.M. Lomaestro and M. Malone, Ann. Pharmacother., 29, 1263 (1995).
- C. Cereser, J. Guichard, J. Drai, E. Bannier, I. Garcia, S. Boget, P. Parvaz and A. Revol, J. Chromatogr. B, 752, 123 (2001).
- T.P. Dalton, H.G. Shertzer and A. Puga, Ann. Rev. Pharmacol. Toxicol., 39, 67 (1999).
- K. Ogawa, Antiox Redox Signaling, 7, 973 (2005).
- A. Meister and M.E. Anderson, Ann. Rev. Biochem., 52, 711 (1983).
- H. Khan, S.J. Jan, Hashmatullah, M.F. Khan, K.A. Khan, Asim-urRehman and A. Wahab, Pak. J. Pharm. Sci., 23, 188 (2010).
- G.K. Balendiran, R. Dabur and D. Fraser, Cell Biochem. Funct., 22, 343 (2004).
- J. Vitecek, J. Petrlova, J. Petrek, V. Adam, D. Potesil, L. Havel, R. Mikelova, L. Trnkova and R. Kizek, Electrochim. Acta, 51, 5087 (2006).
- S.J.S. Flora, G. Flora and G. Saxena, In eds.: S.B. Cascas and J. Sordo, Environmental Occurrence, Health Effects and Management of Lead Poisoning, Lead Chemistry, Analytical Aspects, Environmental Impacts and Health Effects, Netherlands: Elsevier Publication, pp. 158-228 (2006).
- G.L. Ellman, Arch. Biochem. Biophys., 82, 70 (1959).
- A. Pastore, F. Piemonte, M. Locatelli,A.L. Russo, L.M. Gaeta, G. Tozzi and G. Federici, Clin. Chem., 47, 1467 (2003).
- H. Khan, M.F. Khan, A.U. Rehman and N. Ullah, Pak. J. Pharm. Sci., 24, 123 (2011).
- B.P. Ketchel, C.A. Heid, G.L. Wood, M.J. Miller, A.G. Mott, R.J. Anderson and G.J. Salamo, Appl. Optics, 38, 6159 (1999).
- P.J. Meunier, C. Roux and E. Seeman, New Engl. J. Med., 350, 459 (2004).
- H. Zahi, Pubmed., 42, 98 (2000).
- M.E.J. Curzon, Biol. Trace Elem. Res., 3, 309 (1981).
- R. Lide David, CRC Hand Book of Chemistry and Physics, CRC Press: Boca Raton FL, edn. 83, pp. 439-496 (2002).
- H. Khan, M.F. Khan, R.Z. Paracha and U. Hashmat, Gomal Univ. J. Res., 26, 1 ( 2010).
- I. Fabrik, J. Kukacka, J. Baloun, I. Sotornik, V. Adam, R. Prusa, D. Vajtr, P. Babula and R. Kizek, Electroanalysis, 21, 650 (2009).
References
B.M. Lomaestro and M. Malone, Ann. Pharmacother., 29, 1263 (1995).
C. Cereser, J. Guichard, J. Drai, E. Bannier, I. Garcia, S. Boget, P. Parvaz and A. Revol, J. Chromatogr. B, 752, 123 (2001).
T.P. Dalton, H.G. Shertzer and A. Puga, Ann. Rev. Pharmacol. Toxicol., 39, 67 (1999).
K. Ogawa, Antiox Redox Signaling, 7, 973 (2005).
A. Meister and M.E. Anderson, Ann. Rev. Biochem., 52, 711 (1983).
H. Khan, S.J. Jan, Hashmatullah, M.F. Khan, K.A. Khan, Asim-urRehman and A. Wahab, Pak. J. Pharm. Sci., 23, 188 (2010).
G.K. Balendiran, R. Dabur and D. Fraser, Cell Biochem. Funct., 22, 343 (2004).
J. Vitecek, J. Petrlova, J. Petrek, V. Adam, D. Potesil, L. Havel, R. Mikelova, L. Trnkova and R. Kizek, Electrochim. Acta, 51, 5087 (2006).
S.J.S. Flora, G. Flora and G. Saxena, In eds.: S.B. Cascas and J. Sordo, Environmental Occurrence, Health Effects and Management of Lead Poisoning, Lead Chemistry, Analytical Aspects, Environmental Impacts and Health Effects, Netherlands: Elsevier Publication, pp. 158-228 (2006).
G.L. Ellman, Arch. Biochem. Biophys., 82, 70 (1959).
A. Pastore, F. Piemonte, M. Locatelli,A.L. Russo, L.M. Gaeta, G. Tozzi and G. Federici, Clin. Chem., 47, 1467 (2003).
H. Khan, M.F. Khan, A.U. Rehman and N. Ullah, Pak. J. Pharm. Sci., 24, 123 (2011).
B.P. Ketchel, C.A. Heid, G.L. Wood, M.J. Miller, A.G. Mott, R.J. Anderson and G.J. Salamo, Appl. Optics, 38, 6159 (1999).
P.J. Meunier, C. Roux and E. Seeman, New Engl. J. Med., 350, 459 (2004).
H. Zahi, Pubmed., 42, 98 (2000).
M.E.J. Curzon, Biol. Trace Elem. Res., 3, 309 (1981).
R. Lide David, CRC Hand Book of Chemistry and Physics, CRC Press: Boca Raton FL, edn. 83, pp. 439-496 (2002).
H. Khan, M.F. Khan, R.Z. Paracha and U. Hashmat, Gomal Univ. J. Res., 26, 1 ( 2010).
I. Fabrik, J. Kukacka, J. Baloun, I. Sotornik, V. Adam, R. Prusa, D. Vajtr, P. Babula and R. Kizek, Electroanalysis, 21, 650 (2009).