Effect of Polyethylene Glycol on the Synthesis of Tyr-Arg Dipeptide by a-Chymotrypsin in an Organic Medium

Y. Hirano; Y. Nagao

doi:10.14233/ajchem.2013.13873

Issue

Vol. 25 No. 6 (2013): Vol 25 Issue 6

Issue Published : December 26, 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

Effect of Polyethylene Glycol on the Synthesis of Tyr-Arg Dipeptide by a-Chymotrypsin in an Organic Medium

https://doi.org/10.14233/ajchem.2013.13873

Y. Hirano

Department of Chemistry and Materials Engineering, Faculty of Chemistry Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan

Y. Nagao

Department of Applied Chemistry, Faculty of Engineering, Osaka Institute of Technology, 5-16-1 Ohmiya, Asahi-ku, Osaka 535-8585, Japan

Corresponding Author(s) : Y. Hirano

yhirano@kansai-u.ac.jp

Asian Journal of Chemistry, Vol. 25 No. 6 (2013): Vol 25 Issue 6
Article Published : January 9, 2013

Abstract

The enzymatic peptide synthesis in an organic medium was investigated using a-chymotrypsin in the presence of poly(ethylene glycol) (PEG). The added PEG enhanced the activity of a-chymotrypsin for the synthesis of the peptide Z-Tyr-Arg(NO₂)-OBzl.. The activity of a-chymotrypsin in the PEG-added system was higher than in a PEG-free system.

Keywords

Enzymatic peptide synthesis Michaelis-Menten constant Non-modified enzyme Poly(ethylene glycol) Tyr-Arg (Kyotorphin) α-Chymotrypsin

Hirano, Y., & Nagao, Y. (2013). Effect of Polyethylene Glycol on the Synthesis of Tyr-Arg Dipeptide by a-Chymotrypsin in an Organic Medium. Asian Journal of Chemistry, 25(6), 3401–3404. https://doi.org/10.14233/ajchem.2013.13873

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References

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P. Clapés, C. Morán and M.R. Infante, Biotechnol. Bioeng., 63, 333 (1999).
K. Nakanishi, T. Kamikubo and R. Matsumoto, Biotechnology, 3, 459 (1985).
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References

A.M. Klibanov, Chemtech, 16, 354 (1986).

A. Zaks and A.M. Klibanov, Proc. Nat. Acad. Sci. USA, 82, 3192 (1985).

K. Nakanishi, Y. Kimura and and R. Matsuno, Biotechnology, 4, 452 (1986).

J.S. Dordick, Enzym. Microbiol. Technol., 11, 194 (1989).

P. Lozano, D. Combes, J.L. Iborra and A. Manjon, Biotechnol. Lett., 15, 1223 (1993).

H. Waldmann and D. Sebastian, Chem. Rev., 94, 911 (1994).

R.L. Fandino, I. Gill and E.N. Vulfson, Biotechnol. Bioeng., 43, 1024 (1994).

H. Kitano, Y. Maeda, M. Yamamoto and R. Izumida, Macromol. Chem. Phys., 197, 4173 (1996).

P. Clapés, J.L. Torres and P. Adlercreutz, Bioorg. Med. Chem., 3, 245 (1995).

J.M. Cabral, M.R. Aires-Barros, H. Pinheiro and D.M. Prazeres, J. Biotechnol., 59, 133 (1997).

K. Kawashiro, H. Sugahara, S. Sugiyama and H. Hayashi, Biotechnol. Bioeng., 43, 26 (1997).

U. Eichhorn, A.S. Bommarius, K. Drauz and H.-D. Jakubke, J. Peptide Sci., 3, 245 (1997).

U. Eichhorn, K. Beck-Piotraschke, R. Schaaf and H.-D. Jakubke, J. Peptide Sci., 3, 261 (1997).

C. Lombard, J. Saulnier and J.M. Wallach, Protein Peptide Lett., 12, 621 (1994).

H. Sekizaki, K. Itoh, E. Toyota and K. Tanizawa, Chem. Pharm. Bull., 44, 1585 (1996).

H. Sekizaki, K. Itoh, E. Toyota and K. Tanizawa, Amino Acids, 17, 285 (1999).

P. Clapés, C. Morán and M.R. Infante, Biotechnol. Bioeng., 63, 333 (1999).

K. Nakanishi, T. Kamikubo and R. Matsumoto, Biotechnology, 3, 459 (1985).

H. Sekizaki, K. Itoh, A. Shibuya, E. Toyota, M. Kojoma and K. Tanizawa, Chem. Pharm. Bull., 56, 688 (2008).

K. Yoshinaga, S.G. Shefer and J.M. Harris, J. Bioactive Compatible Polym., 2, 49 (1987).

Issue

Vol. 25 No. 6 (2013): Vol 25 Issue 6

Effect of Polyethylene Glycol on the Synthesis of Tyr-Arg Dipeptide by a-Chymotrypsin in an Organic Medium

Corresponding Author(s) : Y. Hirano

Abstract

Keywords

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