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Study on Fingerprints of Different Organs of Arabidopsis thaliana by Using UPLC/ESI-Q-TOF MS
Corresponding Author(s) : Gang Bai
Asian Journal of Chemistry,
Vol. 25 No. 6 (2013): Vol 25 Issue 6
Abstract
To investigate the fingerprint of A. thaliana and compare those of the four different organs (rosette leaves, stems, flowers and stem leaves), an ultra performance liquid chromatography coupled with electrospray ionization quadrupole/time of flight mass spectrometry (UPLC/ESI-Q-TOF MS) was employed. Compounds were separated on the C18 column using a gradient elution of acetonitrile and 0.1 % formic acid in water. The principal components analysis was applied for data treatment. Clear discrimination was obtained in the score plot, providing the difference in chemical characteristic of different organs. Twenty compounds including flavone glycosides, oxylipins, aliphatic acids and others were identified based on their high resolution mass spectra and the MS/MS fragmentation pathways. It is concluded that the several compounds (oxophytodienoid acid, sn-2-O-(dinoroxophytodienoyl) monogalactosyl monoglyceride, kaempferitrin, robinin, arabidopside E and arabidopside A) were significant markers in the different organs of A. thaliana.
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- V. Arbona, D.J. Iglesias, M. Talon and A. Gomez-Cadenas, J. Agric. Food Chem., 57, 7338 (2009).
- E. Grata, J. Boccard, G. Glauser, P.A. Carrupt, E.E. Farmer, J.L. Wolfender and S. Rudaz, J. Sep. Sci., 30, 2268 (2007).
- M. Stobiecki, A. Skirycz, L. Kerhoas, P. Kachlicki, D. Muth, J. Einhorn and B. Mueller-Roeber, Metabolomics, 2, 197 (2006).
- O. Fiehn, J. Kopka, R.N. Trethewey and L. Willmitzer, Anal. Chem., 72, 3573 (2000).
- E. Grata, J. Boccard, D. Guillarme, G. Glauser, P.A. Carrupt, E.E. Farmer, J.L. Wolfender and S. Rudaz, J. Chromatogr. B, 871, 261 (2008).
- A. Fukushima, M. Kusano, H. Redestig, M. Arita and K. Saito, BMC Syst. Biol., 5, 1 (2011).
- J.L. Ward, C. Harris, J. Lewis and M.H. Beale, Phytochemistry, 62, 949 (2003).
- R.D. Hall, New Phytol., 169, 453 (2006).
- I.S. Lurie and S.G. Toske, J. Chromatogr. A, 1188, 322 (2008).
- R.S. Plumb, M.D. Jones, P.D. Rainville and J.K. Nicholson, J. Chromatogr. Sci., 46, 193 (2008).
- R.t'Kindt, L. De Veylder, M. Storme, D. Deforce and J. Van Bocxlaer, J. Chromatogr. B, 871, 37 (2008).
- P. Kachlicki, J. Einhorn, D. Muth, L. Kerhoas and M. Stobiecki, J. Mass Spectrom., 43, 572 (2008).
- R. Nakabayashi, M. Kusano, M. Kobayashi, T. Tohge, K. Yonekura-Sakakibara, N. Kogure, M. Yamazaki, M. Kitajima, K. Saito and H. Takayama, Phytochemistry, 70, 1017 (2009).
- A. Petsalo, J. Jalonen and A. Tolonen, J. Chromatogr. A, 1112, 224 (2006).
- S.J. Bloor and S. Abrahams, Phytochemistry, 59, 343 (2002).
- L.O. Regasini, J.C. Vellosa, D.H. Silva, M. Furlan, O.M. de Oliveira, N.M. Khalil, I.L. Brunetti, M.C. Young, E.J. Barreiro and V.S. Bolzani, Phytochemistry, 69, 1739 (2008).
- C. Reinbothe, A. Springer, I. Samol and S. Reinbothe, FEBS J., 276, 4666 (2009).
- I. Prost, S. Dhondt, G. Rothe, J. Vicente, M.J. Rodriguez, N. Kift, F. Carbonne, G. Griffiths, M.T. Esquerre-Tugaye, S. Rosahl, C. Castresana, M. Hamberg and J. Fournier, Plant Physiol., 139, 1902 (2005).
- G. Glauser, E. Grata, S. Rudaz and J.L. Wolfender, Rapid Commun. Mass Spectrom., 22, 3154 (2008).
- M.A. Perez and M.A. Nussbaum, J. Biomech., 36, 1225 (2003).
- M.K. Reid and K.L. Spencer, Environ. Pollut., 157, 2275 (2009).
References
V. Arbona, D.J. Iglesias, M. Talon and A. Gomez-Cadenas, J. Agric. Food Chem., 57, 7338 (2009).
E. Grata, J. Boccard, G. Glauser, P.A. Carrupt, E.E. Farmer, J.L. Wolfender and S. Rudaz, J. Sep. Sci., 30, 2268 (2007).
M. Stobiecki, A. Skirycz, L. Kerhoas, P. Kachlicki, D. Muth, J. Einhorn and B. Mueller-Roeber, Metabolomics, 2, 197 (2006).
O. Fiehn, J. Kopka, R.N. Trethewey and L. Willmitzer, Anal. Chem., 72, 3573 (2000).
E. Grata, J. Boccard, D. Guillarme, G. Glauser, P.A. Carrupt, E.E. Farmer, J.L. Wolfender and S. Rudaz, J. Chromatogr. B, 871, 261 (2008).
A. Fukushima, M. Kusano, H. Redestig, M. Arita and K. Saito, BMC Syst. Biol., 5, 1 (2011).
J.L. Ward, C. Harris, J. Lewis and M.H. Beale, Phytochemistry, 62, 949 (2003).
R.D. Hall, New Phytol., 169, 453 (2006).
I.S. Lurie and S.G. Toske, J. Chromatogr. A, 1188, 322 (2008).
R.S. Plumb, M.D. Jones, P.D. Rainville and J.K. Nicholson, J. Chromatogr. Sci., 46, 193 (2008).
R.t'Kindt, L. De Veylder, M. Storme, D. Deforce and J. Van Bocxlaer, J. Chromatogr. B, 871, 37 (2008).
P. Kachlicki, J. Einhorn, D. Muth, L. Kerhoas and M. Stobiecki, J. Mass Spectrom., 43, 572 (2008).
R. Nakabayashi, M. Kusano, M. Kobayashi, T. Tohge, K. Yonekura-Sakakibara, N. Kogure, M. Yamazaki, M. Kitajima, K. Saito and H. Takayama, Phytochemistry, 70, 1017 (2009).
A. Petsalo, J. Jalonen and A. Tolonen, J. Chromatogr. A, 1112, 224 (2006).
S.J. Bloor and S. Abrahams, Phytochemistry, 59, 343 (2002).
L.O. Regasini, J.C. Vellosa, D.H. Silva, M. Furlan, O.M. de Oliveira, N.M. Khalil, I.L. Brunetti, M.C. Young, E.J. Barreiro and V.S. Bolzani, Phytochemistry, 69, 1739 (2008).
C. Reinbothe, A. Springer, I. Samol and S. Reinbothe, FEBS J., 276, 4666 (2009).
I. Prost, S. Dhondt, G. Rothe, J. Vicente, M.J. Rodriguez, N. Kift, F. Carbonne, G. Griffiths, M.T. Esquerre-Tugaye, S. Rosahl, C. Castresana, M. Hamberg and J. Fournier, Plant Physiol., 139, 1902 (2005).
G. Glauser, E. Grata, S. Rudaz and J.L. Wolfender, Rapid Commun. Mass Spectrom., 22, 3154 (2008).
M.A. Perez and M.A. Nussbaum, J. Biomech., 36, 1225 (2003).
M.K. Reid and K.L. Spencer, Environ. Pollut., 157, 2275 (2009).