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Simultaneous Determination of Thiamine and Pyridoxine in Bulk Drug and Vitamin Tablet by HPLC-UV Method
Corresponding Author(s) : Yucel Kadioglu
Asian Journal of Chemistry,
Vol. 25 No. 6 (2013): Vol 25 Issue 6
Abstract
Thiamine and pyridoxine which are also called vitamin B1 and B6, play crucial role in biochemical reactions in human body. HPLC-UV method for the quantitative determination of thiamine and pyridoxine in bulk drug and vitamin tablet were developed and validated in the present study. The mobile phase consisted of a mixture of water (pH: 3.5) and methanol (91:9, v/v) flowing at a flow rate of 1.0 mL/min. Calibration curve was linear between concentration range of 1-40 μg/mL. The intraday and interday precision and accuracy were between 0.8-5.4 % and 0.2-6.6 % for both analytes, respectively. Besides, the method was successfully applied to the simultaneous determination of thiamine and pyridoxine in vitamin tablet.
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- T. Perez-Ruiz, C. Martinez-Lozano, A. Sanz and A. Guillen, J. Pharm. Biomed. Anal., 34, 551 (2004).
- R. Losa, M.I. Sierra,A. Fernandez, D. Blanco and J.M. Buesa, J. Pharm. Biomed. Anal., 37, 1025 (2005).
- P.L.M. Lynch and I.S. Young, J. Chromatogr. A., 881, 267 (2000).
- Monograph, Alternat. Med. Rev., 6, 87 (2001).
- D. Ozdemir and E. Dinc, Chem. Pharm. Bull., 52, 810 (2004).
- P.O. Barrales, A.D. Vidal, M.L.F. de Cordova and A.M. Diaz, J. Pharm. Biomed. Anal., 25, 619 (2001).
- Y.H. Chen, J.F. Zhang, F.S. Tian and Y.H. Zhang, Asian J. Chem., 23, 2169 (2011).
- F.R.P. Rocha, F.O. Fatibello and B.F. Reis, Talanta, 59, 191 (2000).
- E. Dinc, G. Kokdil and F. Onur, J. Pharm. Biomed. Anal., 22, 915 (2000).
- V. Such, J. Traveset, R. Gonzalo and E. Gelpi,Anal. Chem., 52, 412 (1980).
- E. Dinc and D. Baleanu, J. Pharm. Biomed. Anal., 31, 969 (2003).
- E. Dinc and D. Baleanu, Talanta, 59, 707 (2003).
- M. Barary, M. Abdel-Hamid, E. Hassan and M. Elsayed, Pharmazie, 41, 483 (1986).
- L. Garcia, S. Blazquez, M.P. San Andres and S. Vera, Anal. Chim. Acta, 434, 193 (2001).
- A.R. Pires, A.N. Araujo, J.A. Lopes, M. Conceiçao and B.S.M. Montenegro, Anal. Lett., 42, 1923 (2009).
- S.A. Halvatzis and M. Timotheou-Potamia, Anal. Chim. Acta, 227, 405 (1989).
- R.L. Kirchmeier and R.P. Upton, J. Pharm. Sci., 67, 1444 (1978).
- T. Cannella and G. Bichi, Boll. Chim. Farm., 122, 205 (1983).
- F.L. Lam, I.J. Holcomb, S.A. Fusari, J. AOAC Int., 67, 1007 (1984).
- N. Ramos-Martos, F. Aguirre-Gomez,A. Molina-Diaz and L.F. CapitanVallvey, J. AOAC Int., 84, 676 (2001).
- M.L. Marszall,A. Lebiedzinska, W. Czarnowski and P. Szefer, J. Chromatogr. A, 1094, 91 (2005).
- K. Li, Biomed. Chromatogr., 16, 504 (2002).
- N. Yantih, D. Widowati and W. Tiwiaryani, Can. J. Sci. Ind. Res., 2, 269 (2011).
- R. Admidzic, J. Brboric, O. Cudina and S. Vladimirov, J. Serb. Chem. Soc., 70, 1229 (2005).
- O. Hassan and M.J. Chee, Malay. J. Anal. Sci., 7, 251 (2001).
- K. Swan-Choo and T.E.-Siong., Malays. J. Nut., 2, 49 (1996).
- C.K. Markopoulou, K.A. Kagkadis and J.E. Koundourellis, J. Pharm. Biomed. Anal., 30, 1403 (2002).
- Validation of Analytical Procedures, Proceedings of the International Conference on Harmonization (ICH). Commission of the European Communities, (1996).
References
T. Perez-Ruiz, C. Martinez-Lozano, A. Sanz and A. Guillen, J. Pharm. Biomed. Anal., 34, 551 (2004).
R. Losa, M.I. Sierra,A. Fernandez, D. Blanco and J.M. Buesa, J. Pharm. Biomed. Anal., 37, 1025 (2005).
P.L.M. Lynch and I.S. Young, J. Chromatogr. A., 881, 267 (2000).
Monograph, Alternat. Med. Rev., 6, 87 (2001).
D. Ozdemir and E. Dinc, Chem. Pharm. Bull., 52, 810 (2004).
P.O. Barrales, A.D. Vidal, M.L.F. de Cordova and A.M. Diaz, J. Pharm. Biomed. Anal., 25, 619 (2001).
Y.H. Chen, J.F. Zhang, F.S. Tian and Y.H. Zhang, Asian J. Chem., 23, 2169 (2011).
F.R.P. Rocha, F.O. Fatibello and B.F. Reis, Talanta, 59, 191 (2000).
E. Dinc, G. Kokdil and F. Onur, J. Pharm. Biomed. Anal., 22, 915 (2000).
V. Such, J. Traveset, R. Gonzalo and E. Gelpi,Anal. Chem., 52, 412 (1980).
E. Dinc and D. Baleanu, J. Pharm. Biomed. Anal., 31, 969 (2003).
E. Dinc and D. Baleanu, Talanta, 59, 707 (2003).
M. Barary, M. Abdel-Hamid, E. Hassan and M. Elsayed, Pharmazie, 41, 483 (1986).
L. Garcia, S. Blazquez, M.P. San Andres and S. Vera, Anal. Chim. Acta, 434, 193 (2001).
A.R. Pires, A.N. Araujo, J.A. Lopes, M. Conceiçao and B.S.M. Montenegro, Anal. Lett., 42, 1923 (2009).
S.A. Halvatzis and M. Timotheou-Potamia, Anal. Chim. Acta, 227, 405 (1989).
R.L. Kirchmeier and R.P. Upton, J. Pharm. Sci., 67, 1444 (1978).
T. Cannella and G. Bichi, Boll. Chim. Farm., 122, 205 (1983).
F.L. Lam, I.J. Holcomb, S.A. Fusari, J. AOAC Int., 67, 1007 (1984).
N. Ramos-Martos, F. Aguirre-Gomez,A. Molina-Diaz and L.F. CapitanVallvey, J. AOAC Int., 84, 676 (2001).
M.L. Marszall,A. Lebiedzinska, W. Czarnowski and P. Szefer, J. Chromatogr. A, 1094, 91 (2005).
K. Li, Biomed. Chromatogr., 16, 504 (2002).
N. Yantih, D. Widowati and W. Tiwiaryani, Can. J. Sci. Ind. Res., 2, 269 (2011).
R. Admidzic, J. Brboric, O. Cudina and S. Vladimirov, J. Serb. Chem. Soc., 70, 1229 (2005).
O. Hassan and M.J. Chee, Malay. J. Anal. Sci., 7, 251 (2001).
K. Swan-Choo and T.E.-Siong., Malays. J. Nut., 2, 49 (1996).
C.K. Markopoulou, K.A. Kagkadis and J.E. Koundourellis, J. Pharm. Biomed. Anal., 30, 1403 (2002).
Validation of Analytical Procedures, Proceedings of the International Conference on Harmonization (ICH). Commission of the European Communities, (1996).