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An Anion Exchange Monolithic Cartridge for Extraction and Analysis of Oleanolic Acid from Oldenlandia diffusa
Corresponding Author(s) : K.H. Row
Asian Journal of Chemistry,
Vol. 25 No. 6 (2013): Vol 25 Issue 6
Abstract
An anion exchange monolithic cartridge was synthesized and used for extraction and analysis of oleanolic acid from Oldenlandia diffusa. The analysis was performed on HPLC with an eluting solution consisting of methanol-water-acetic acid (88:12:0.5, v/v/v, pH = 5.8) and a C18 column. An ultrasonic-assisted process was applied to accelerate the extraction process, which was obviously increasing the extraction efficiency. Under the optimum condition, good linearity was obtained in a range of 50-2000 μg mL-1. The intra-day and inter-day were less than 4.8 and 5.7 % and recoveries were ranging from 98.3-104.5 %. This method can be used for the extraction and analysis of drugs from natural plants due to its simplicity and reliability.
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- H.Y. Cheung, S.H. Cheung, M.L. Law and W.P. Lai, J. Chromatogr. B, 834, 195 (2006).
- P.K. Wu, W.C.S. Tai, Z.T. Liang, Z.Z. Zhao and W.L.W. Hsiao, Life Sci., 85, 113 (2009).
- Y. Zhang, Y. Chen, C. Fan, W. Ye and J. Luo, Fitoterapia, 81, 515 (2010).
- G.J. Xu, L.S. Xu and Z.T. Wang, Fujian Science and Technology Press, Fuzhou (1997).
- S. Gupta, D. Zhang, J. Yi and J. Shao, J. Herb. Pharmacother, 4, 21 (2004).
- C. Li, X. Xue, D. Zhou, F. Zhang, Q. Xu, L. Ren and X. Liang, J. Pharm. Biomed. Anal., 48, 205 (2008).
- S.H. Kim, B.Z. Ahn and S.Y. Ryu, Phytother. Res., 12, 553 (1998).
- T. Zhu and K.H. Row, Chromatographia, 71, 981 (2010).
- J. Liu, T. Zhu, M. Tian and K.H. Row, Asian J. Chem., 21, 6285 (2009).
- A. Gelencser, G. Kiss, Z. Krivacsy, Z. Varga-Puchony and J. Hlavay, J. Chromatogr. A, 693, 217 (1995).
- G. Yang, H. Liu, Y. Zhang, S. Wang, J. Yin, B. Yin and Y. Chen, J. Chromatogr. A, 1129, 231 (2006).
- T. Zhu and K. H. Row, J. Liquid Chromatogr. Rel. Technol., 32, 1423 (2009).
- T. Zhu and K.H. Row, Chromatographia, 69, 1477 (2009).
- Z. Liang, Z. Jiang, D.W. Fong and Z. Zhao, J. Food Drug Anal., 17, 69 (2009).
References
H.Y. Cheung, S.H. Cheung, M.L. Law and W.P. Lai, J. Chromatogr. B, 834, 195 (2006).
P.K. Wu, W.C.S. Tai, Z.T. Liang, Z.Z. Zhao and W.L.W. Hsiao, Life Sci., 85, 113 (2009).
Y. Zhang, Y. Chen, C. Fan, W. Ye and J. Luo, Fitoterapia, 81, 515 (2010).
G.J. Xu, L.S. Xu and Z.T. Wang, Fujian Science and Technology Press, Fuzhou (1997).
S. Gupta, D. Zhang, J. Yi and J. Shao, J. Herb. Pharmacother, 4, 21 (2004).
C. Li, X. Xue, D. Zhou, F. Zhang, Q. Xu, L. Ren and X. Liang, J. Pharm. Biomed. Anal., 48, 205 (2008).
S.H. Kim, B.Z. Ahn and S.Y. Ryu, Phytother. Res., 12, 553 (1998).
T. Zhu and K.H. Row, Chromatographia, 71, 981 (2010).
J. Liu, T. Zhu, M. Tian and K.H. Row, Asian J. Chem., 21, 6285 (2009).
A. Gelencser, G. Kiss, Z. Krivacsy, Z. Varga-Puchony and J. Hlavay, J. Chromatogr. A, 693, 217 (1995).
G. Yang, H. Liu, Y. Zhang, S. Wang, J. Yin, B. Yin and Y. Chen, J. Chromatogr. A, 1129, 231 (2006).
T. Zhu and K. H. Row, J. Liquid Chromatogr. Rel. Technol., 32, 1423 (2009).
T. Zhu and K.H. Row, Chromatographia, 69, 1477 (2009).
Z. Liang, Z. Jiang, D.W. Fong and Z. Zhao, J. Food Drug Anal., 17, 69 (2009).