Effects of Varroa destructor Anderson & Trueman Infestation on Antioxidant Enzymes of Adult Worker Honey Bee (Apis mellifera L.)

Yasar Gülmez; Dursun Kisa; Ilyas Can

doi:10.14233/ajchem.2016.19459

Issue

Vol. 28 No. 3 (2016): Vol 28 Issue 3

Issue Published : December 5, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Effects of Varroa destructor Anderson & Trueman Infestation on Antioxidant Enzymes of Adult Worker Honey Bee (Apis mellifera L.)

https://doi.org/10.14233/ajchem.2016.19459

Yasar Gülmez

Department of Biology, Gaziosmanpasa University, Tokat, Turkey

Dursun Kisa

Department of Biology, Gaziosmanpasa University, Tokat, Turkey

Ilyas Can

Department of Biology, Gaziosmanpasa University, Tokat, Turkey

Corresponding Author(s) : Yasar Gülmez

yasar_gulmez@yahoo.com

Asian Journal of Chemistry, Vol. 28 No. 3 (2016): Vol 28 Issue 3
Article Published : December 5, 2015

Abstract

One of the most important honeybee pathogens is the mite Varroa destructor, which threats honeybee health and incomes from bee keeping. Varroa mite, among other effects, can cause oxidative stress which is indicated by alterations in antioxidant enzyme activity and total protein levels. In this study, activities of catalase, glutathione S-transferase, superoxide dismutase enzymes and quantity of total protein levels were compared in mite infested and mite-free adult worker bees. Superoxide dismutase enzyme activity remarkably increased in mite infested bees as compared to uninfested ones. Catalase enzyme activity was found low in mite infested bees although no significant difference was observed in glutathione S-transferase enzyme activity. Total protein level in mite infested bees was detected lower than uninfested ones. Changes in enzyme activities and total protein levels are resulted from oxidative stress caused by mite infestation.

Keywords

Varroa destructor Honey bees Oxidative stress Antioxidant enzymes

Gülmez, Y., Kisa, D., & Can, I. (2015). Effects of Varroa destructor Anderson & Trueman Infestation on Antioxidant Enzymes of Adult Worker Honey Bee (Apis mellifera L.). Asian Journal of Chemistry, 28(3), 663–665. https://doi.org/10.14233/ajchem.2016.19459

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References

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References

T.C. Webster and K.S. Delaplane, Dadant and Sons, Inc., 280 pp, Hamilton, Illinois (2001).

M.L. Reshi, Y.C. Su and J.-R. Hong, Int. J. Cell Biol., Article ID 467452 (2014); doi:10.1155/2014/467452.

K. Żóltowska, P. Grochla and E. Łopieńska-Biernat, Ann. Parasitol., 52, 283 (2006).

K. Rahman, Clin. Interv. Aging, 2, 219 (2007).

G.F. Weirich, A.M. Collins and V.P. Williams, Apidologie (Celle), 33, 3 (2002); doi:10.1051/apido:2001001.

A.M. Korayem, M.M. Khodairy, A. Abdel-Aal and A.A.M. El-Sonbaty, J. Biol. Earth Sci., 2, B93 (2012).

A.M. Collins, V. Williams and J.D. Evans, Insect Mol. Biol., 13, 141 (2004); doi:10.1111/j.0962-1075.2004.00469.x.

T. Kayis, I. Emre and M. Coskun, Turkish J. Entomol., 36, 463 (2012).

P. Badotra, N.R. Kumar and K. Harjai, J. Global Biosci., 2, 199 (2013).

Z. Lipinski and K. Zoltowska, J. Apic. Res., 44, 126 (2005).

W.F. Beyer Jr. and I. Fridovich, Anal. Biochem., 161, 559 (1987); doi:10.1016/0003-2697(87)90489-1.

H. Aebi, Methods Enzymol., 105, 121 (1984); doi:10.1016/S0076-6879(84)05016-3.

W. Habig, H. Pabst and M.K. Jakoby, J. Biochem., 249, 7130 (1974).

J. Bradford, Anal. Biochem., 72, 248 (1976); doi:10.1016/0003-2697(76)90527-3.

A.J. Nappi, E. Vass, F. Frey and Y. Carton, Eur. J. Cell Biol., 68, 450 (1995).

M.M.A. Whitten, C.B. Mello, S.A.O. Gomes, Y. Nigam, P. Azambuja, E.S. Garcia and N.A. Ratcliffe, Exp. Parasitol., 98, 44 (2001); doi:10.1006/expr.2001.4615.

N. Krishnan and D. Kodrík, J. Insect Physiol., 52, 11 (2006); doi:10.1016/j.jinsphys.2005.08.009.

Y. Kono and I. Fridovich, J. Biol. Chem., 257, 5751 (1982).

S. Ahmad, D.L. Duval, L.C. Weinhold and R.S. Pardini, Insect Biochem., 21, 563 (1991); doi:10.1016/0020-1790(91)90111-Q.

C.M. Mathews, C.B. Summers and G.W. Felton, Arch. Insect Biochem. Physiol., 34, 57 (1997); doi:10.1002/(SICI)1520-6327(1997)34:1<57::AID-ARCH5>3.0.CO;2-T.

C.B. Summers and G.W. Felton, Biochim. Biophys. Acta, 1156, 235 (1993); doi:10.1016/0304-4165(93)90142-U.

J. Hemingway, N.J. Hawkes, L. McCarroll and H. Ranson, Insect Biochem. Mol. Biol., 34, 653 (2004); doi:10.1016/j.ibmb.2004.03.018.

P.T. Bowen-Walker and A. Gunn, Entomol. Exp. Appl., 101, 207 (2001); doi:10.1046/j.1570-7458.2001.00905.x.

C. van Dooremalen, E. Stam, L. Gerritsen, B. Cornelissen, J. van der Steen, F. van Langevelde and T. Blacquiere, J. Insect Physiol., 59, 487 (2013); doi:10.1016/j.jinsphys.2013.02.006.

Issue

Vol. 28 No. 3 (2016): Vol 28 Issue 3

Effects of Varroa destructor Anderson & Trueman Infestation on Antioxidant Enzymes of Adult Worker Honey Bee (Apis mellifera L.)

Corresponding Author(s) : Yasar Gülmez

Abstract

Keywords

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