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Vol. 25 No. 5 (2013): Vol 25 Issue 5

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Spectrophotometric Determination of Isoniazid from Pharmaceutical Preparations Using Natural Aldehyde

https://doi.org/10.14233/ajchem.2013.13444
K.F. Almani
Department of Pharmaceutics, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
M.G.H. Laghari
Department of Pharmaceutics, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
A.H. Memon
Department of Pharmaceutics, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
F.M.A. Rind
Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro, Pakistan
U.R. Mughal
Department of Pharmaceutics, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
M.L. Maheshwari
Department of Pharmaceutics, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
M.Y. Khuhawer
Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro, Pakistan

Corresponding Author(s) : K.F. Almani

falmani@hotmail.com

Asian Journal of Chemistry, Vol. 25 No. 5 (2013): Vol 25 Issue 5

Abstract

A spectrophotometric method has been developed for the micro determination of isoniazid from various pharmaceutical dosage forms. Method is based on a condensation reaction between isoniazid and ethanolic solution of cis-cinnamaldehyde to generate an instant yellow coloured schiff's base derivative of isoniazid. The coloured product formed absorbed in visible region and optimal detector response was obtained at a wavelength of 364 nm with a molar absorptivity of 3.1 × 104 L/mol/cm. The analytical parameters and their effects on the reported systems are investigated. A linear relationship was obtained between the absorbance and the concentration of the derivative which obeyed the Beer's law within 0.5-2.5 μg/mL. The low % relative standard deviation (0.2-1.5) values indicate good precision, high recovery values and accuracy of proposed method.

Keywords

Isoniazid cis-Cinnamaldehyde Spectrophotometry
Almani, K., Laghari, M., Memon, A., Rind, F., Mughal, U., Maheshwari, M., & Khuhawer, M. (2012). Spectrophotometric Determination of Isoniazid from Pharmaceutical Preparations Using Natural Aldehyde. Asian Journal of Chemistry, 25(5), 2522–2526. https://doi.org/10.14233/ajchem.2013.13444
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