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Pharmacokinetic Interaction Between Polyphyllin II and Hyperoside Extracts of Rhizoma Paridis and Hypericum Perforatum After Oral Administration in Beagle Dogs
Corresponding Author(s) : Jian Ni
Asian Journal of Chemistry,
Vol. 25 No. 5 (2013): Vol 25 Issue 5
Abstract
A rapid and specific RP-HPLC-UV method was developed for the analysis of polyphyllin II and hyperoside in beagle dog plasma. The analysis was carried out on a Diamonsil C18 (2) reversed-phase column (250 × 4.6 mm, 5 mm) by isocratic elution with acetonitrile and 0.1 % phosphoric acid (50: 50, v/v). The flow rate was 1.0 mL/min and the detection wavelength was set at 203 nm and 360 nm. Linear responses were obtained for polyphyllin II ranging from 3.3333 to 100 μg/mL and hyperoside ranging from 2 to 60 μg/mL. The intra and interday precisions (RSDs) were less than 9.23 and 9.24 % with the accuracy (%) ranging from 89.67 to 107.25 %. The extraction recovery ranged from 84.97 to 91.32 % with RSD less than 7.381 %. Stability studies showed that two compounds were stable in analytical process. In the pharmacokinetic interaction study, present findings indicate that polyphyllin II and hyperoside have a clear effect on the pharmacokinetic behaviour.
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References
The Pharmacopoeia Commission of PRC, Vol. 215, p. 243 (2010).
Y.H. Hua, S.L. Ma, Z.F. Fu, H.Z. Mou and H. Jiang, Chin. Arch. TCM, 29, 1387 (2011).
L.L. Yan, Y.J. Zhang, W.Y. Gao and S.L. Man, Chin. Trad. Herbal Drugs, 40, 424 (2009).
F. Shan, L.J. Yang, Y.F. Peng, X.R. Kang and Y.H. Ma, Chin. JMAP, 27, 961 (2010).
Z.W. Chen and C. Ma, Acta Pharmacol. Sin., 1, 20 (1999).
W.Q. Wang, C.G. Ma and X.Y. Xu, Acta Pharmacol. Sina, 4, 14 (1996).
J.R. Wei and J. Li, Mod. Chin. Med., 9, 26 (2007).
X.L. Bi, A.L. Xu and W.H. Luo, Chin. J. Exp. Trad. Med. Formulae, 16, 64 (2010).
Y. Ke, J. Ni and J.C. Yao, J. Beijing Univ. Trad. Chin. Med., 8, 555 (2006).