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Vol. 25 No. 4 (2013): Vol 25 Issue 4

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Spectroscopic Techniques Study on the Quenching Mechanism of Cytochrome B5 Affected by Cu(II)

https://doi.org/10.14233/ajchem.2013.13304
Q. Tang
Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian City 116024, Liaoning Province, P.R. China ; Liaoning Key Lab of Bioorganic Chemistry, Dalian University, Dalian City 116622, P.R. China
X.J. Peng
Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian City 116024, Liaoning Province, P.R. China
H.Y. Cao
Liaoning Key Lab of Bioorganic Chemistry, Dalian University, Dalian City 116622, P.R. China
X.F. Zheng
Liaoning Key Lab of Bioorganic Chemistry, Dalian University, Dalian City 116622, P.R. China
J.Y. Wang
Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian City 116024, Liaoning Province, P.R. China

Corresponding Author(s) : X.F. Zheng

dlxfzheng@126.com

Asian Journal of Chemistry, Vol. 25 No. 4 (2013): Vol 25 Issue 4

Abstract

The interaction between Cu2+ and cytochrome b5 (cytb5) has been investigated by multi-spectroscopic techniques. The results show that Cu2+ could interact with cytochrome b5 and induce fluorescence of the latter to be quenched. The quenching mechanism is dominated by dynamic quenching. Synchronous fluorescence and circular dichroism spectra demonstrate that Cu2+ could also influence the micro-circumatance of aromatic amino residues and secondary structure of the protein.

Keywords

Cytochrome B5 Quenching mechanism Spectrosciopic techniques
Tang, Q., Peng, X., Cao, H., Zheng, X., & Wang, J. (2012). Spectroscopic Techniques Study on the Quenching Mechanism of Cytochrome B5 Affected by Cu(II). Asian Journal of Chemistry, 25(4), 2054–2058. https://doi.org/10.14233/ajchem.2013.13304
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