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Vol. 25 No. 4 (2013): Vol 25 Issue 4

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Effect of Mirtazapine on Gastric Oxidative Stress and DNA Injury Created With Methotrexate in Rats

https://doi.org/10.14233/ajchem.2013.13296
Ismail Demiryilmaz
Department of General Surgery, Private Ibn-i Sina Hospital, Kayseri, Turkey
Hulya Uzkeser
Department of Physical Therapy and Rehabilitation, Regional Training and Research Hospital, Erzurum, Turkey
Nihal Cetin
Department of Pharmacology, Ataturk University, Erzurum, Turkey
Ahmet Hacimuftuoglu
Department of Pharmacology, Ataturk University, Erzurum, Turkey
Ebubekir Bakan
Department of Biochemistry, Ataturk University, Erzurum, Turkey
Durdu Altuner
Department of Pharmacology, Rize University Medical Faculty, Rize, Turkey

Corresponding Author(s) : Durdu Altuner

durdualtuner@hotmail.com

Asian Journal of Chemistry, Vol. 25 No. 4 (2013): Vol 25 Issue 4

Abstract

In this study, effect of mirtazapine on gastric oxidative stress and DNA injury created with methotrexate was investigated. Experimental results showed that GSH (nmol/g protein), MDA (μmol/g protein) and MPO (μ/g protein) in the gastric tissue of the control group rats receiving methotrexate are 4.97 ± 0.37, 2.78 ± 0.30 and 3.12 ± 0.18, respectively. GSH, MDA and MPO measurements in the gastric tissue of rats receiving mirtazapine + methotrexate were detected to be 9.23 ± 0.51(p < 0.0001), 1.80 ± 0.31(p < 0.0001) and 1.63 ± 0.25 (p < 0.0001), respectively. GSH, MDA and MPO values in the intact rat group were found 8 ± 0.38 (p < 0,0001), 1.63 ± 0.28 (p < 0.0001) and 1.37 ± 0.21 (p < 0.0001), respectively. In addition, while 8-ohdG/dG quantity that DNA injury product in the control group administered methotrexate was 2.4 ± 0.11 pmol/L, this quantity was 1.3 ± 0.14 pmol/L (p < 0.001), 1.1 ± 0.10 pmol/L (p < 0.001) in mirtazapine and intact group, respectively. As a result, it was seen that mirtazapine prevents increase of oxidative stress and DNA injury created with methotreaxete in the gastric tissue of rat.

Keywords

Mirtazapine Methotrexate Oxidative Stress DNA Injury Rat
Demiryilmaz, I., Uzkeser, H., Cetin, N., Hacimuftuoglu, A., Bakan, E., & Altuner, D. (2012). Effect of Mirtazapine on Gastric Oxidative Stress and DNA Injury Created With Methotrexate in Rats. Asian Journal of Chemistry, 25(4), 2047–2050. https://doi.org/10.14233/ajchem.2013.13296
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