Issue

Vol. 25 No. 4 (2013): Vol 25 Issue 4

Copyright (c) 2013 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS

https://doi.org/10.14233/ajchem.2013.13204
Rezeng Caidan
Department of Chinese Medicine Chemistry, Beijing University of Chinese Medicine, Beijing, P.R. China ; The research Center of Chinese and Tibetan Medicine, Medicine College of Qinghai University, Xining, P.R. China
Limao Cairang
Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, P.R. China ; Department of Chemistry Science, College of Nationality Normal, Qinghai Normal University, Xining, P.R. China
Liubin
Department of Chinese Medicine Chemistry, Beijing University of Chinese Medicine, Beijing, P.R. China
Suo Yourui
Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, P.R. China

Corresponding Author(s) : Liubin

liubinyn67@163.com

Asian Journal of Chemistry, Vol. 25 No. 4 (2013): Vol 25 Issue 4

Abstract

The aim of this study was to optimize the microwave extraction conditions of Rubus niveus Thunb. oil from Qinghai-Tibetan plateau via Box-Behnken design of RSM and then analyze the composition of oil by HPLC-MS/MS using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl-4-toluene-sulfonate as labeling reagent. The optimum microwave extraction conditions were as follows: irradiation power 437 W, extraction temperature 75 ºC and extraction time 12 min. Under these optimized conditions, the maximum oil extraction yield was 5.83 %. All fatty acid derivates had excellent linear responses with correlation coefficients greater than 0.9990. Detection limits (at signal-to-noise of 3:1) were 18.42 to 32.39 pmol. The results indicate that the predominant fatty acids (C10-C22) were 16:0, 18:2, 18:3, 18:0 and 22:0 in total fatty acids. And the unsaturated fatty acids were 18:3, 18:2, 18:1 and 20:4 and reached up to 40.54 % of the total mass contents of fatty acids (C10-C22) in Rubus niveus Thunb. from Qinghai-Tibetan plateau.

Keywords

Oil extraction Response surface methodology Fatty acids HPLC-MS Rubus niveus Thunb fruit
Caidan, R., Cairang, L., Liubin, ., & Yourui, S. (2012). Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS. Asian Journal of Chemistry, 25(4), 1866–1870. https://doi.org/10.14233/ajchem.2013.13204
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. T. Flynn and D.H. Lorence, Bishop Mus. Occas. Pap., 56, 5 (1998).
  2. C. Parmar and M.K. Kaushal, Wild Fruits, Kalyani Publishers, New Delhi, India, pp. 88-91 (1982).
  3. W.W. Christie, High-Performance Liquid Chromatography and Lipids, Pergamon Press (English), p. 156 (1987).
  4. K. Asakura, H. Ohrui and H. Meguro, Analyst, 118, 765 (1993).
  5. J.C. Marr, L.M. McDowell and M.A. Quilliam, Nat. Toxins, 5, 302 (1988).
  6. T. Iwata, T. Hirose, M. Nakamura and M. Yamaguchi, Analyst, 119, 1747 (1994).
  7. T. Toyo'oka, Anal. Chim. Acta, 465, 111 (2002).
  8. Y.P. Gao, Y.Y. Fu, Z.W. Sun, L. Kang, Z.Y. Ji, H.H. Zhao, L. Zhang and J.M. You, J. Qufu. Normal Univ., 37, 63 (2011).
  9. H.P. Li, G.Q. Zhao, S.T. Niu and Y.G. Luan, Comp. Mater. Sci., 38, 561 (2007).
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0