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Vol. 25 No. 3 (2013): Vol 25 Issue 3

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Spectrophotometric and Conductometric Investigation of Azithromycin with Ca2+ Cation and Determination of Azithromycin in Dosage Form by Spectrophotometric Method

https://doi.org/10.14233/ajchem.2013.12660
Razieh Sanavi Khoshnood
Department of Chemistry, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Toktam Laal Zakaria
Department of Chemistry, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Zarrin Eshaghi
Department of Chemistry, Payame Noor University, Mashhad, Iran

Corresponding Author(s) : Razieh Sanavi Khoshnood

rskhoshnood@yahoo.com

Asian Journal of Chemistry, Vol. 25 No. 3 (2013): Vol 25 Issue 3

Abstract

The interaction of the azithromycin with Ca(NO3)2.4H2O in methanol has been investigated by conductometric method to determine the number of complexes formed. The conductance data show that the stoichiometry of complex between azithromycin and Ca2+ is 2:1 [M:L2] in pure MeOH. The interaction of the azithromycin with Ca2+ was studied by spectrophotometric method under equilibrium conditions. Calcium is a biometal in human body. The interaction of azithromycin with Ca2+ salt has been found to form one complex with metal to ligand composition of 1:2. The value of (Kf = 3.7 × 104 M-2) was calculated from the absorption spectra for Ca2+-azithromycin complex at room temperature. Spectrophotometric method was developed and used to determine azithromycin-calcium at a lmax of 260 nm. The influence of pH, time, temperature and interferences has been tested. Also, the calibration graph of complex at the presence of HCl was linear (R2 = 0.980) over the range of (10-4-10-2 M). This simple method has been applied to the determination of azithromycin in dosage form.

Keywords

Azithromycin Ca2 cation Spectrophotometric Drug interaction Conductometric
Sanavi Khoshnood, R., Laal Zakaria, T., & Eshaghi, Z. (2012). Spectrophotometric and Conductometric Investigation of Azithromycin with Ca2+ Cation and Determination of Azithromycin in Dosage Form by Spectrophotometric Method. Asian Journal of Chemistry, 25(3), 1237–1240. https://doi.org/10.14233/ajchem.2013.12660
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References
  1. M. Rachidi and J. Elharti, Anal. Lett., 40, 53 (2007).
  2. B. Nigovic and B. Simunic, J. Pharm. Biomed. Anal., 32, 197 (2003).
  3. P. Zubata, R. Ceresole, M.A. Rosasco and M.T. Pizzorono, J. Pharm. Biomed. Anal., 27, 833 (2002).
  4. D. Li, D.G. Wang, Z.Y. Nie, Y.Q. Zhu and Y.H. Sun, Chin. J. Clin. Pharmacol., 12, 355 (2003).
  5. Y.H. Wu, X.B. Ji and S.S. Hu, Bioelectrochemistry, 64, 91 (2004).
  6. C. Taninaka, H. Ohtani, E. Hanada, H. Kotaki, H. Sato and T. Iga, J. Chromatogr. B, 738, 405 (2000).
  7. Y.H. Kim, J.V. Pothuluri and C.E. Cerniglia, J. Pharm. Biomed. Anal., 38, 390 (2005).
  8. G.W. Amsden and C.L. Gray, J. Antimicrob. Chemother., 47, 61 (2001).
  9. K.A. Rodvold, L.H. Danziger and M.H. Gotfried, Antimicrob. Agents Chemother., 47, 2450 (2003).
  10. F. Kees, S. Spangler and M. Wellenhofer, J. Chromatogr. A, 812, 284 (1998).
  11. R. Gandhi, C.L. Kaul and R. Panchagnula, J. Pharm. Biomed. Anal., 23, 1073 (2000).
  12. J.L. Davis, S.Y. Gardner, S.L. Jones, B.A. Schwabenton and M.G. Papich, J. Vet. Pharmacol. Therap., 25, 99 (2002).
  13. R.M. Shepard, G.S. Duthu, R.A. Ferraina and M.A. Mullins, J. Chromatogr. B, 565, 321 (1991).
  14. G. Bahrami and B. Mohammadi, J. Chromatogr. B, 830, 355 (2006).
  15. J.S. Torano and H.J. Guchelaar, J. Chromatogr. B, 720, 89 (1998).
  16. E. Wilmsa, H. Trumpie, W. Veenendaal and D. Touw, J. Chromatogr. B, 814, 37 (2005).
  17. G. Bahrami, S. Mirzaeei and A. Kiani, J. Chromatogr. B., 820, 277 (2005).
  18. A.W. El-Rjoob, J. Al-Mustafa, Z. Taha and M. Abous, Jordan J. Chem., 3, 199 (2008).
  19. H.F. Dos Santos, E.S. Xavier, M.C. Zerner and W.B. De Almeida, J. Mol. Struct., 527, 193 (2000).
  20. L. Lambs and G. Berthon, Inorg. Chim. Acta, 151, 33 (1988).
  21. M. Jezowska-Bojczuk, L. Lambs, H. Kozlowski and G. Berthon, Inorg. Chem., 32, 428 (1993).
  22. J.M. De Siqueira, S. Carvalho, E.B. Paniago, L. Tosi and H. Beraldo, J. Pharm. Sci., 83, 291 (1993).
  23. F.C. Machado, C. Domicheli, A. Garner-Suilerot and H. Beraldo, J. Inorg. Biochem., 60, 163 (1995).
  24. S.V. De Mello-Matos, H. Beraldo and J. Braldo, J. Braz. Chem. Soc., 6, 405 (1995).
  25. A. Bravo and J.R. Anacona, J. Coord. Chem., 44, 173 (1998).
  26. M.M. Shoukry, A.K. Abdel Hadi and W.M. Hosny, Synth. React. Inorg. Met.-Org., 25, 45 (1995).
  27. M.J. Zaworotko, H.H. Hammud, I. Abbas, V. Kravtsov and M.S. Masoud, J. Coord. Chem., 59, 65 (2006).
  28. N.P. Gensmantel, P. Poctor and M.I. Page, J. Chem. Soc. Perkin Trans. II, 1725 (1980).
  29. M. Hakimi, A. Nezhadali and A. Naemi, E-J. Chem., 5, 551 (2008).
  30. G.H. Rounaghi, M.H. Arbab Zavvar, F. Boosaeedi and R.S. Khoshnood, J. Incl. Phenom. Macrocycl. Chem., 47, 101 (2003).
  31. N.V. Chavgun, I.S. Zaitseva, E.N. Kabakova and N.V. Bodarew, Russ. J. Gen. Chem. 68, 1209 (1988).
  32. M. Rachidi, J. Elaharti, K. Digua, Y. Cherrah and A. Bouklouze, Anal. Lett., 39, 1917 (2006).
  33. S.S. Sabnis, N.D. Dhavale, V.Y. Jahav and S.V. Gandhi, Spectrochim. Acta A, 69, 849 (2008).
  34. R. Gandhi, O. Pillai, R. Thilagavathi, B. Gopalakrishnan, C.L. Kaul and R. Panchagnula, Eur. J. Pharm. Sci., 16, 175 (2002).
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