Copyright (c) 2013 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Role of Free Radicals on Retinitis Pigmentosa
Corresponding Author(s) : Serap Yalin
Asian Journal of Chemistry,
Vol. 25 No. 3 (2013): Vol 25 Issue 3
Abstract
Retinitis pigmentosa is a retinal disease that leads to blindness. It has an unclear etiology and no known cure available in literature. The aim of this study is to study the role of free radicals on retinitis pigmentosa. This study was conducted on a test group composed of subjects who have been diagnosed with retinitis pigmentosa for ten years; and a control group of healthy individuals. To analyze the role of reactive oxygen products on retinal cell apoptosis, malondialdehyde (MDA) as a product of lipid peroxidation was measured. Also, to study the protectiveness of antioxidant enzymes over the retinal cells from apoptosis; activities pertaining to superoxide dismutase, catalase and glutathione peroxidase have been analyzed. The malondialdehyde values at the patient group have been found to be significantly higher than those at the control group. Antioxidant enzyme activities were found to be significantly lower in patient groups. Similar pattern of change was observed between patient and control group for all parameters in females and males. The malondialdehyde value was significantly higher and other parameters were lower in patient group according to controls. Results obtained from this research indicate that radical damage was high and antioxidant enzymes were low in retinitis pigmentosa. We may interpret these results to mean that exposure to free radical or antioxidant enzyme insufficiency may be some of the factors that trigger retinitis pigmentosa.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- K. Komeima, B.S. Rogers and P.A. Campochiaro, Proc. Natl. Acad. Sci. (USA), 103, 11300 (2006).
- K. Komeima, B.S. Rogers and P.A. Campochiaro, J. Cell Physiol., 213, 809 (2007).
- U. Shinichi, K. Keiichi and S.Y. Lee, Mol. Therap., 17, 778 (2009).
- D.Y. Yu, S.J. Cringle and E.N. Su, Invest. Ophthalmol. Vis. Sci., 41, 3999 (2000).
- E.N. Marieb, Essentials of Human Anatomy & Physiology, Addison, p. 558 (2000).
- K. Yagi, Methods Mol. Biol., 108, 107 (1998).
- Y. Sun, L.W. Oberley and L.Ying, Clin. Chem., 34, 497 (1988).
- H. Aebi, Methods Enzymol., 105, 121 (1984).
- D. Paglia and W.N. Valentine, J. Lab. Clin. Med., 70, 158 (1967).
- C. Punzo, K. Kornacker and C.L. Cepko, Nat. Neurosci., 12, 44 (2009).
- H.W. Flynn, S.B. Bressler, G.C. Brown, T. Meredith, C.D. Regillo and R.D. Isernhagen, Retina and Vitreous, Basic and Clinical Science Course, Section 12, Foundation of the American Academy of Ophthalmology, p. 367 (2000).
- S.P. Dagier, L.A. Sullivan and J.A. Rodriquez, Behav. Brain. Sci., 18, 452 (1990).
- U. Acharya, S. Patel and E. Koundakjian, Nat. Rev. Mol. Cell. Biol., 4, 397 (2003).
- A.J. Hardcastle, D.L. Thiselton and L. Van Maldergem,Am. J. Hum. Genet., 64, 1210 (1999).
- R.J. Carmody, A.J. Mc Gowan and T.G. Cotter, Exp. Cell Res., 248, 520 (1999).
- B. Halliwell, W.M.C. Gutteridge, Free Radicals in Biology and Medicine, Oxford Medicine Press, p. 246 (1999).
- J.S. Jr. Zigler, Arch. Biochem. Biophys,. 225, 149 (1983).
- Z. Wu, J. Cell. Physiol., 209, 996 (2006).
- P. Ahuja-Jensen, S. Johnsen-Soriano, S. Ahuja, F. Bosch-Morell, M. SanchoTello, F.J. Romero, M. Abrahamson and T. van Veen, Neuroreport, 18, 797 (2007).
- R. Fernandes and T. Cotter, Biochem. Pharmacol., 48, 675 (1994).
- S. Usui, Mol. Therap., 17, 778 (2009).
- V. Geromel, N. Kadhom, I. Cebalos-Picot, O. Ouari,A. Polidori,A. Munnich, A. Rötig and P. Rustin, Hum. Mol. Genet., 10, 1221 (2001).
References
K. Komeima, B.S. Rogers and P.A. Campochiaro, Proc. Natl. Acad. Sci. (USA), 103, 11300 (2006).
K. Komeima, B.S. Rogers and P.A. Campochiaro, J. Cell Physiol., 213, 809 (2007).
U. Shinichi, K. Keiichi and S.Y. Lee, Mol. Therap., 17, 778 (2009).
D.Y. Yu, S.J. Cringle and E.N. Su, Invest. Ophthalmol. Vis. Sci., 41, 3999 (2000).
E.N. Marieb, Essentials of Human Anatomy & Physiology, Addison, p. 558 (2000).
K. Yagi, Methods Mol. Biol., 108, 107 (1998).
Y. Sun, L.W. Oberley and L.Ying, Clin. Chem., 34, 497 (1988).
H. Aebi, Methods Enzymol., 105, 121 (1984).
D. Paglia and W.N. Valentine, J. Lab. Clin. Med., 70, 158 (1967).
C. Punzo, K. Kornacker and C.L. Cepko, Nat. Neurosci., 12, 44 (2009).
H.W. Flynn, S.B. Bressler, G.C. Brown, T. Meredith, C.D. Regillo and R.D. Isernhagen, Retina and Vitreous, Basic and Clinical Science Course, Section 12, Foundation of the American Academy of Ophthalmology, p. 367 (2000).
S.P. Dagier, L.A. Sullivan and J.A. Rodriquez, Behav. Brain. Sci., 18, 452 (1990).
U. Acharya, S. Patel and E. Koundakjian, Nat. Rev. Mol. Cell. Biol., 4, 397 (2003).
A.J. Hardcastle, D.L. Thiselton and L. Van Maldergem,Am. J. Hum. Genet., 64, 1210 (1999).
R.J. Carmody, A.J. Mc Gowan and T.G. Cotter, Exp. Cell Res., 248, 520 (1999).
B. Halliwell, W.M.C. Gutteridge, Free Radicals in Biology and Medicine, Oxford Medicine Press, p. 246 (1999).
J.S. Jr. Zigler, Arch. Biochem. Biophys,. 225, 149 (1983).
Z. Wu, J. Cell. Physiol., 209, 996 (2006).
P. Ahuja-Jensen, S. Johnsen-Soriano, S. Ahuja, F. Bosch-Morell, M. SanchoTello, F.J. Romero, M. Abrahamson and T. van Veen, Neuroreport, 18, 797 (2007).
R. Fernandes and T. Cotter, Biochem. Pharmacol., 48, 675 (1994).
S. Usui, Mol. Therap., 17, 778 (2009).
V. Geromel, N. Kadhom, I. Cebalos-Picot, O. Ouari,A. Polidori,A. Munnich, A. Rötig and P. Rustin, Hum. Mol. Genet., 10, 1221 (2001).