Copyright (c) 2013 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Determination of Enantiomeric Purity of 2-Piperidinemethanamine by HPLC Combined With Pre-Column Derivation
Corresponding Author(s) : Hang Song
Asian Journal of Chemistry,
Vol. 25 No. 3 (2013): Vol 25 Issue 3
Abstract
A new HPLC method has been developed and validated for determination of enantiomeric purity of 2-piperidinemethanamine within a short run time of less than 10 min. The method was based on pre-column derivation of 2-piperidinemethanamine with 3,5-dinitrobenzoic acid and complete separation of enantiomers has been achieved on a CHI-DMB analytical column (250 mm × 4.6 mm) using n-hexane: ethanol (85:15 v/v) as mobile phase at a flow rate of 1.0 mL min-1 under UV photodiode-array detector detection. Then the effects of mobile phase and temperature on enantioselectivity were further evaluated. The method was validated with respect to precision, accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ) and robustness. The recoveries were between 99.1 and 102.3 % with percentage relative standard deviation less than 1.17 %. The LOD and LOQ for first enantiomer were 13.7 and 46.3 μg mL-1 and for second enantiomer were 15.2 and 51.4 μg mL-1, respectively. This method is expected to be accurate, stable, rapid and sensitive for determination of the enantiomeric purity of 2-piperidinemethanamine in bulk samples.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- A.J. Rao, V.K. Rao, P.V. Rao, B.S. Krishna, C.N. Raju and S.K. Ghosh, Int. J. Pharm. Biol. Sci., 1, 1 (2010).
- H. Tuerkmen, T. Pape, F.E. Hahn and B. Cetinkaya, Organometallics, 27, 571 (2008).
- M. Fujita, K. Chiba, Y. Tominaga and K. Hino, Chem. Pharm. Bull., 46, 787 (1998).
- Z.X. Wang, Y.Q. Li and B.R. Guntoori, US Patent 20050059825 (2005).
- H.K.J. Hall, O.E. Ekechukwu, A.J. Deutschmann and C. Rose, Polym. Bull., 3, 375 (1980).
- K. Morikawa, M. Honda, K. Endoh, T. Matsumoto, K. Akamatsu, H. Mitsui and M. Koizumi, J. Pharm. Sci., 79, 750 (1990).
- Y.W. Zhang, S. Yao, H. Zeng and H. Song, Curr. Pharm. Anal., 6, 114 (2010).
- J. Lu and A.M. Rustum, J. Chromatogr. Sci., 46, 466 (2008).
- Y.W. Zhang, D. Yang, S. Yao, Y. Cao and H. Song, J. Chromatogr. Sci., 49, 67 (2011).
- Q. Sun and S.V. Olesik, J. Chromatogr. B, 745, 159 (2000).
- G.S. Ding, Y. Liu, R.Z. Cong and J.D. Wang, Talanta, 62, 997 (2004).
- R.N. Rao, A.N. Raju and D. Nagaraju, J. Pharm. Biomed. Anal., 42, 347 (2006).
- A. Berthod, W. Li and D.W. Armstrong, Anal. Chem., 64, 873 (1992).
- B. Loun and D.S. Hage, Anal. Chem., 66, 3814 (1994).
- R.W. Stringham and J.A. Blackwell, Anal. Chem., 69, 1414 (1997).
References
A.J. Rao, V.K. Rao, P.V. Rao, B.S. Krishna, C.N. Raju and S.K. Ghosh, Int. J. Pharm. Biol. Sci., 1, 1 (2010).
H. Tuerkmen, T. Pape, F.E. Hahn and B. Cetinkaya, Organometallics, 27, 571 (2008).
M. Fujita, K. Chiba, Y. Tominaga and K. Hino, Chem. Pharm. Bull., 46, 787 (1998).
Z.X. Wang, Y.Q. Li and B.R. Guntoori, US Patent 20050059825 (2005).
H.K.J. Hall, O.E. Ekechukwu, A.J. Deutschmann and C. Rose, Polym. Bull., 3, 375 (1980).
K. Morikawa, M. Honda, K. Endoh, T. Matsumoto, K. Akamatsu, H. Mitsui and M. Koizumi, J. Pharm. Sci., 79, 750 (1990).
Y.W. Zhang, S. Yao, H. Zeng and H. Song, Curr. Pharm. Anal., 6, 114 (2010).
J. Lu and A.M. Rustum, J. Chromatogr. Sci., 46, 466 (2008).
Y.W. Zhang, D. Yang, S. Yao, Y. Cao and H. Song, J. Chromatogr. Sci., 49, 67 (2011).
Q. Sun and S.V. Olesik, J. Chromatogr. B, 745, 159 (2000).
G.S. Ding, Y. Liu, R.Z. Cong and J.D. Wang, Talanta, 62, 997 (2004).
R.N. Rao, A.N. Raju and D. Nagaraju, J. Pharm. Biomed. Anal., 42, 347 (2006).
A. Berthod, W. Li and D.W. Armstrong, Anal. Chem., 64, 873 (1992).
B. Loun and D.S. Hage, Anal. Chem., 66, 3814 (1994).
R.W. Stringham and J.A. Blackwell, Anal. Chem., 69, 1414 (1997).