Gas Chromatographic Determination of α-Keto Acids in Pharmaceutical Preparation Using 1,2-Propylenediamine as Derivatizing Reagent

K.U. Abbasi; M.Y. Khuhawar; K.P. Mahar; Arfana Mallah; A. Nawaz Siyal

doi:10.14233/ajchem.2013.12367

Issue

Vol. 25 No. 3 (2013): Vol 25 Issue 3

Issue Published : October 8, 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

Gas Chromatographic Determination of α-Keto Acids in Pharmaceutical Preparation Using 1,2-Propylenediamine as Derivatizing Reagent

https://doi.org/10.14233/ajchem.2013.12367

K.U. Abbasi

Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan

M.Y. Khuhawar

Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan

K.P. Mahar

Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan

Arfana Mallah

Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan

A. Nawaz Siyal

Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan

Corresponding Author(s) : M.Y. Khuhawar

mykhuhawar@usindh.edu.pk; khalidamahar@yahoo.com

Asian Journal of Chemistry, Vol. 25 No. 3 (2013): Vol 25 Issue 3
Article Published : October 8, 2012

Abstract

Analytical procedure has been developed for the gas chromatographic separation and determination of a-keto acids: pyruvic acid, 2-ketobutyric acid, 3-methyl-2-oxobutyric acid, 3-methyl-2-oxovaleric acid, 4-methyl-2-oxovaleric acid, 2-ketohexanoic acid and phenylpyruvic acid after derivatization with 1,2-propylenediamine from the column HP-5 (30 × 0.32 mm i.d). The detection was made by flame ionization. Linear calibration curves were obtained within 9.0-84 μg/mL and detection limits within 3.0-5.6 μg/mL of each a-keto acids. A pharmaceutical preparation ketosteril was analyzed for phenylpyruvic acid, 3-methyl-2-oxovaleric acid, 4-methyl-2-oxovaleric acid and 3-methyl-2-oxobutyric acid and the found amounts correlated with the labeled values. The extraction recovery from pharmaceutical preparation tested by standard addition of these compounds was calculated to be 94.0-96.8 % with RSD 0.1-0.8 %. A number of pharmaceutical additives and amino acids added did not affect the determination of a-keto acids with relative error within ± 3 %.

Keywords

Gas Chromatography α-Keto acids 1,2-Propylenediamine Drug analysis

Abbasi, K., Khuhawar, M., Mahar, K., Mallah, A., & Nawaz Siyal, A. (2012). Gas Chromatographic Determination of α-Keto Acids in Pharmaceutical Preparation Using 1,2-Propylenediamine as Derivatizing Reagent. Asian Journal of Chemistry, 25(3), 1211–1215. https://doi.org/10.14233/ajchem.2013.12367

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M. Petrarulo, M. Marangella and F. Linari, Clin. Chim. Acta, 196, 17 (1991).

M. Lange and M. Malyusz, J. Chromatogr. B., 662, 97 (1994).

M.S.M. Ardawi, Clin. Sci., 81, 603 (1991).

P.R. de Vasconcelos, M.G. Kettlewell and D.H. Williamson, Clin. Sci., 72, 683 (1987).

U. Fauth, W. HeimLich and I. Puente-Gonzalez, Phase, 17, 178 (1990).

A.C. Mclellan, S.A. Philips and P.J. Thornay, Anal. Biochem., 206, 17 (1992).

I. Nemet, L. Varga-Defterdarovic and Z. Turk, Clin. Biochem., 37, 875 (2004).

K. Pailla, F.B.-Cynober, C. Aussel, J.-P. De Bandt and L. Cynober, Clin. Chem., 46, 848 (2000).

W. Base, C. Barsigian, A. Schaeffer, E. Shaw, J. Martinez and W.C. Maddrey, Hepatology, 7, 324 (1987).

B.C. Hemming and C.J. Gubler, Anal. Biochem., 92, 31 (1979).

S. Hara, Y. Takemori, M. Yamaguchi and M. Nakamura, J. Chromatogr. B, 344, 33 (1985).

J.E. Spikner and J.C. Towne, Anal. Chem., 34, 1468 (1962).

P. De Schepper and G. Paramentier, Biochim. Biophys. Acta, 28, 507 (1958).

J. Dancis, J. Hutzler and M. Levitz, Biochem. Biophys. Acta, 78, 85 (1963).

M. Nakamura, S. Hara, M. Yamaguchi, Y. Takemori and Y. Ohkura, Chem. Pharm. Bull., 35, 687 (1987).

X. Ji, C. Yang, J. Yang and X. Peng, Fenxi Huaxue, 33, 527 (2005).

X. Fu, M. Kimura, M. Lga and S. Yamaguchi, J. Chromatogr. B., 758, 87 (2001).

L.I. Woolf, C. Hasinoff and A. Ferry, J. Chromatogr., 231, 237 (1982).

E.T. Urbansky and W.J. Bashe, J. Chromatogr. A., 867, 143 (2000).

F. Rocchiccioli, J.P. Leroux and P. Cartier, Biomed. Mass Spectrom., 8, 160 (1981).

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H.P. Schwartz, I.E. Kar and D.M. Bler, Anal. Biochem., 108, 360 (1980).

R.J. Early, J.R. Thompson, T. McAllister, T.W. Fenton and R.J. Christopherson, J. Chromatogr. B, 310, 1 (1984).

K. Tanaka, D.G. Hine, A. West-Dull and T.B. Lynn, Clin. Chem., 26, 1839 (1980).

A.A. Fernandez, S.C. Kalhan, N.G. Njoroge and G.S. Matousek, Biomed. Envioron.-Mass Spectrom., 13, 569 (1986).

D.L. Hachey, B.W. Patterson, P.J. Reeds and L.J. Elsas, Anal. Chem., 63, 919 (1991).

R. Navarro-Gonzalez,A. Negron-Mendoza and G. Albarran, J. Chromatogr. A, 587, 247 (1991).

A. Frigerio, P. Martelli, K.M. Baker and P.A. Biondi, J. Chromatogr. A, 81, 139 (1973).

Issue

Vol. 25 No. 3 (2013): Vol 25 Issue 3

Gas Chromatographic Determination of α-Keto Acids in Pharmaceutical Preparation Using 1,2-Propylenediamine as Derivatizing Reagent

Corresponding Author(s) : M.Y. Khuhawar

Abstract

Keywords

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