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Breeding of Producing L-Valine Strain Brevibacterium flavum NJ112 by Protoplast Fusion Technology
Corresponding Author(s) : Huanmin Liu
Asian Journal of Chemistry,
Vol. 25 No. 3 (2013): Vol 25 Issue 3
Abstract
With Brevibacterium flavum NVT1103 (Leul, a-ABhr, 2-TAhr, SGr) and JVHK597 (Leu–, Ile–, Metl, a-ABr, 2-TAr) as parental strains, conditions of two parental strains' protoplast formation and regeneration were groped about. On this basis, a series of protoplast fusion experiment were conducted to investigated the influence of fusion temperature in the fusion process, poly(ethylene glycol) concentration and the fusion time. Under unoptimized conditions, the L-valine production of the strain NJ112 derivative in flasks was 45.6 g/L.
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- C. Sanfeng and L. Dehu, Moden Microbial Genetics, Chemical Industry Press, Beijing, pp. 308-316 (2003).
- Z.J. Tan, Q.M. Xiao and K.Y. Xiao, Biotechnology, 2, 35 (2003).
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- H.M. Liu and W.G. Zhang, Afr. J. Biotechnol., 9, 21 (2010).
- H.M. Liu, X.Y. Ge and W.G. Zhang, Asian J. Chem., 23, 4483 (2011).
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References
C. Sanfeng and L. Dehu, Moden Microbial Genetics, Chemical Industry Press, Beijing, pp. 308-316 (2003).
Z.J. Tan, Q.M. Xiao and K.Y. Xiao, Biotechnology, 2, 35 (2003).
D. Lianxiang and L. Fuping, Microbiology Experimental Techniques, China Light Industry Press, Beijing, pp. 210-211 (2005).
L. Ferenczy, F. Kevei and J. Zsolt, Nature, 248, 793 (1974).
R. Uegaki, T. Fuiimori, H. Kaneko, K. Kato and M. Noguchi, Agric. Biol. Chem., 43, 1149 (1979).
H.M. Liu and W.G. Zhang, Afr. J. Biotechnol., 9, 21 (2010).
H.M. Liu, X.Y. Ge and W.G. Zhang, Asian J. Chem., 23, 4483 (2011).
R.E. Buchanan, Bergey's Manual of Srytematic Bacteriology, Science Press, Beijing, p. 869 (1984).
K. Fink, R.E. Cline and R.M. Fink, Anal. Chem., 35, 389 (1963).
S.K. Mandal and S.K. Majumdar, Sci. Culture, 36, 556 (1970).
H.M. Liu, X.Y. Ge and W.G. Zhang, Asian J. Chem., 23, 2151 (2011).
L. Zaizi, Fundamental Theory of Biochemical Engineering, Chemical Industry Press, Beijing, pp. 18-19 (1999).
Z. Yingfu, K. Zhenrong and D. Shengmiao, China Brewing, 2, 16 (2006).