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Vol. 25 No. 2 (2013): Vol 25 Issue 2

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Studying the Effective Concentration of RGDS on RGDS-Poly(DL-Lactic Acid)

https://doi.org/10.14233/ajchem.2013.12878
Ya Fu
Key Laboratory of Biorheological Science and Technology, Ministry of Education; Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, P.R. China ; College of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, P.R. China
Meina Huang
Key Laboratory of Biorheological Science and Technology, Ministry of Education; Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, P.R. China ; National Engineering Research Center for Magnesium Alloy, Department of Materials Science and Engineering, Chongqing University, Chongqing 400030, P.R. China
Yanfeng Luo
Key Laboratory of Biorheological Science and Technology, Ministry of Education; Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, P.R. China
Chengbo Hu
Key Laboratory of Biorheological Science and Technology, Ministry of Education; Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, P.R. China
Yuanliang Wang
Key Laboratory of Biorheological Science and Technology, Ministry of Education; Research center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, P.R. China

Corresponding Author(s) : Yuanliang Wang

wyl@cqu.edu.cn

Asian Journal of Chemistry, Vol. 25 No. 2 (2013): Vol 25 Issue 2

Abstract

RGDS-PDLLA is a biomimetic material obtained by covalently incorporating RGDS adhesion peptide. RGDS-PDLLA was prepared on basis of BDPLA (butanediamine-grafted polyactic acid). In present work, we prepared RGDS-PDLLA with gradient concentration RGDS and evaluated the cytocompatibility of gradient RGDS-PDLLA and took a novel method for studying the effective concentration of RGDS after grafting on BDPLA. When the concentration of RGDS in the RGDS-grafted-PDLLA (RGDS-PDLLA) varied from 0.142 to 0.302 μmol/g, cell cytocompatibility of osteoblasts was altered depending on the concentration of RGDS. 0.1 μmol/g RGDS on RGDS-PDLLA (RGDS-PDLLA) membrane can effectively promoted cell attachment, cell spreading. The concentration of RGDS increased from 0.142 to 0.302 μmol/g, the adhesion of osteoblasts on RGDS-PDLLA membrane increased slightly. However, we can find that when the RGDS increased to 2.42 μmol/g, the cell on films increased. These results suggest that the cell adhesion is sensitive to the RGDS concentration. By the study, we can confirm the optimal concentration of RGDS on RGDS-PDLLA.

Keywords

RGDS-PDLLA Effective concentration Arg-gly-asp-ser (RGDS) Adhesion
Fu, Y., Huang, M., Luo, Y., Hu, C., & Wang, Y. (2012). Studying the Effective Concentration of RGDS on RGDS-Poly(DL-Lactic Acid). Asian Journal of Chemistry, 25(2), 763–766. https://doi.org/10.14233/ajchem.2013.12878
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