Copyright (c) 2013 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Extraction of Cantharidin from Mylabris phalerata Pallas with Compound Enzyme and HPLC Analysis
Corresponding Author(s) : Xizhen Ge
Asian Journal of Chemistry,
Vol. 25 No. 2 (2013): Vol 25 Issue 2
Abstract
Cantharidin is a monoterpene anhydride released from beetle Mylabris phalerata Pallas or Mylabris cichorii Linnaeus as a defensive substance showing versatile pharmacological activities especially anticancer. Now efficient extraction methods for cantharidin have been established so far. Here, four extraction methods, including hydrochloric acid, NaOH solution, mixed extraction and compound enzyme (cellulase and pectinase) methods were compared with traditional Chinese pharmacopoeia extraction method. A quantitative HPLC method was developed for the estimation of cantharidin in Mylabris phalerata extract. The compound enzyme method was revealed to present the highest yield of cantharidin among these methods, followed in order by hydrochloric acid method, mixed extraction method, Chinese pharmacopoeia method and NaOH solution method. The HPLC conditions comprise: diamonsil C18 (250 mm × 4.6 mm, 5 μm), flow rate of 1.0 mL/min, detection wavelength of 273 nm, column temperature at 25 ºC, mobile phase with acetonitrile: water = 40:60. Under these conditions, cantharidin could be well extracted from Mylabris phalerata.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- G.S. Wang, J. Ethnopharmacol., 26, 147 (1989).
- Chinese Pharmacopoeia Committee, State Pharmacopoeia of the People's Republic of China [S], Beijing: Chemical Industry Press, p. 233 (2005).
- V. Bertini, M. Calderone, C. Fragai and E. Talluri, J. Med. Chem., 52, 4838 (2009).
- O.D. Laerum and O.H. Iversen, Cancer Res., 32, 1463 (1972).
- K. Bonness, I.V. Aragon, B. Rutland, S. Ofori-Acquah, N.M. Dean and R.E. Honkanen, Mol. Cancer Ther., 5, 2727 (2006).
- D. Liu and Z. Chen, Anticancer Agents Med Chem., 9, 392 (2009).
- Q. Liu, J.W. Chen, X. Li and B.C. Cai, J. Hainan Med. Coll., 17, 582 (2011).
- S. Chen, X.H. Xing, J.J. Huang and M.S. Xu,Enzyme Microbiol. Technol., 48, 100 (2011).
- A. Mehdinia, M. Asiabi, A. Jabbari and S.M. Abtahi, J. Chromatogr. B, 879, 2897 (2011).
- C.M. Wei, Y. Teng, B.J. Wang, X.M. Zhang, G.Y. Yuan, X.Y. Liu, R. Li and R.C. Guo, J. Chromatogr. B, 879, 1741 (2011).
References
G.S. Wang, J. Ethnopharmacol., 26, 147 (1989).
Chinese Pharmacopoeia Committee, State Pharmacopoeia of the People's Republic of China [S], Beijing: Chemical Industry Press, p. 233 (2005).
V. Bertini, M. Calderone, C. Fragai and E. Talluri, J. Med. Chem., 52, 4838 (2009).
O.D. Laerum and O.H. Iversen, Cancer Res., 32, 1463 (1972).
K. Bonness, I.V. Aragon, B. Rutland, S. Ofori-Acquah, N.M. Dean and R.E. Honkanen, Mol. Cancer Ther., 5, 2727 (2006).
D. Liu and Z. Chen, Anticancer Agents Med Chem., 9, 392 (2009).
Q. Liu, J.W. Chen, X. Li and B.C. Cai, J. Hainan Med. Coll., 17, 582 (2011).
S. Chen, X.H. Xing, J.J. Huang and M.S. Xu,Enzyme Microbiol. Technol., 48, 100 (2011).
A. Mehdinia, M. Asiabi, A. Jabbari and S.M. Abtahi, J. Chromatogr. B, 879, 2897 (2011).
C.M. Wei, Y. Teng, B.J. Wang, X.M. Zhang, G.Y. Yuan, X.Y. Liu, R. Li and R.C. Guo, J. Chromatogr. B, 879, 1741 (2011).