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Phytochemical Analysis of Curcumin from Turmeric by RP-HPLC
Corresponding Author(s) : J.Y. Ma
Asian Journal of Chemistry,
Vol. 25 No. 2 (2013): Vol 25 Issue 2
Abstract
Curcumin is isolated from turmeric. The effect of solvent composition on the total extraction yield of turmeric has been investigated using the dipping method. HPLC-UV diode-array (DAD), electrospray mass spectrometry (ESI) and TLC have been used simultaneously to analyze curcumin in a fresh turmeric extract. From the results, it is evident that the percentage of curcumin extracted from turmeric by pure methanol was higher than any aqueous methanol composition; although the total extraction yield was the highest in 100 % water.
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- J.C. Tilak, M. Banerjee, H. Mohan and T.P. Devasagayam, Phytother. Res., 18, 798 (2004).
- X.G. He, L.Z. Lin, L.Z. Lian and M. Lindermaier, J. Chromatogr. A, 818, 127 (1998).
- X. Sun, C, Gao, W. Cao, X. Yang and E. Wang, J. Chromatogr., 962, 117 (2002).
- B.B. Aggarwal, A. Kumar and A.C. Bharti, Anticancer Res., 23, 363 (2003).
- S. Gilda, M. Kanitkar, R. Bhonde and A. Paradkar, Food Sci. Technol., 43, 59 (2010).
- S. Pandit, H.J. Kim, J.E. Kim and J.G. Jeon, Food Chem., 126, 1565 (2011).
- R.K. Singh, D. Rai, D. Yadav, A. Bhargava, J. Balzarini and E.D. Clercq, Eur. J. Med. Chem., 45, 1078 (2010).
- W. Thongchai, B. Liawruangrath and S. Liawruangrath, Food Chem., 112, 494 (2009).
- B.B. Aggarwal and K.B. Harikumar, Int. J. Biochem. Cell Biol., 41, 40 (2009).
- G.K. Jayaprakasha, L.J.M. Rao and K.K. Sakariah, Food Sci. Technol., 16, 533 (2005).
- H.H. Tomesen and J. Karlsen, J. Chromatogr., 259, 367 (1983).
- H.D. Jang, K.S. Chang, Y.S. Huang, C.L. Hsu, S.H. Lee and M.S. Su, Food Chem., 103, 749 (2007).
- P.Y. Zhan, X.H. Zeng, H.M. Zhang and H.H. Li, Food Chem., 129, 700 (2011).
- J.H. Lee and M.G. Choung, Food Chem., 124, 1217 (2011).
- P. Anand, S.G. Thomas, A.B. Kunnumakkara, C. Sundaram, K.B. Harikumar, B. Sung, S.T. Tharakan, K. Misra, I.K. Priyadarsini, K.N. Rajasekharan and B.B. Aggarwal, Biochem. Pharm., 76, 1590 (2008).
References
J.C. Tilak, M. Banerjee, H. Mohan and T.P. Devasagayam, Phytother. Res., 18, 798 (2004).
X.G. He, L.Z. Lin, L.Z. Lian and M. Lindermaier, J. Chromatogr. A, 818, 127 (1998).
X. Sun, C, Gao, W. Cao, X. Yang and E. Wang, J. Chromatogr., 962, 117 (2002).
B.B. Aggarwal, A. Kumar and A.C. Bharti, Anticancer Res., 23, 363 (2003).
S. Gilda, M. Kanitkar, R. Bhonde and A. Paradkar, Food Sci. Technol., 43, 59 (2010).
S. Pandit, H.J. Kim, J.E. Kim and J.G. Jeon, Food Chem., 126, 1565 (2011).
R.K. Singh, D. Rai, D. Yadav, A. Bhargava, J. Balzarini and E.D. Clercq, Eur. J. Med. Chem., 45, 1078 (2010).
W. Thongchai, B. Liawruangrath and S. Liawruangrath, Food Chem., 112, 494 (2009).
B.B. Aggarwal and K.B. Harikumar, Int. J. Biochem. Cell Biol., 41, 40 (2009).
G.K. Jayaprakasha, L.J.M. Rao and K.K. Sakariah, Food Sci. Technol., 16, 533 (2005).
H.H. Tomesen and J. Karlsen, J. Chromatogr., 259, 367 (1983).
H.D. Jang, K.S. Chang, Y.S. Huang, C.L. Hsu, S.H. Lee and M.S. Su, Food Chem., 103, 749 (2007).
P.Y. Zhan, X.H. Zeng, H.M. Zhang and H.H. Li, Food Chem., 129, 700 (2011).
J.H. Lee and M.G. Choung, Food Chem., 124, 1217 (2011).
P. Anand, S.G. Thomas, A.B. Kunnumakkara, C. Sundaram, K.B. Harikumar, B. Sung, S.T. Tharakan, K. Misra, I.K. Priyadarsini, K.N. Rajasekharan and B.B. Aggarwal, Biochem. Pharm., 76, 1590 (2008).