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Vol. 25 No. 1 (2013): Vol 25 Issue 1

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Role of Different Cytokinins and Auxins for Micropropagation, Callogenesis and Plant Regeneration in Lilium (Lilium longiflorum)

https://doi.org/10.14233/ajchem.2013.13140
Aamir Ali
Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan
Saira Yasmin
Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan
Rabia Shaheen Niazi
Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan
Abdul Majid
Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan
Naima Huma Naveed
Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan

Corresponding Author(s) : Aamir Ali

aamirali73@hotmail.com

Asian Journal of Chemistry, Vol. 25 No. 1 (2013): Vol 25 Issue 1

Abstract

In vitro propagation of Lilium longiflorum was carried out to explore its potential for micropropagation and callogenesis using meristem and twin scales explants and to study the influence of different concentrations and combinations of various phytoharmones along with varying physical factors like temperature and light. MS medium supplemented with 2 mg/L of BAP was found to be best for shoot formation from meristem, with 84 % shoot formation within 12 days of meristem inoculation while in case of twin scales, 88 % shoot initiation response was obtained on MS medium containing 1.5 mg/L of BAP with 0.5 mg/L of NAA after 12 days of inoculation. Maximum shoot multiplication response was obtained at 3 mg/L of Kinetin with an average of 4.8 shoots per culture vial after 8.2 days of inoculation. The best in vitro rooting response was obtained on MS medium supplemented with 1.5 mg/L of NAA i.e. an average of 5.2 roots were formed after 12 days of inoculation with an average root length of 1.76 cm. When well developed in vitro plants were shifted for hardening on different media (sand, silt, peat, sand+ peat and sand+ silt+ peat in the ratio of 1:1, 1:1:1 respectively). 100 % Hardening response was recorded on Peat. Maximum callus induction response (88 %) was obtained from twin scale explant after 9.8 days of inoculation on MS supplemented with 2.0 mg/L of NAA + 0.25 mg/L of BAP. A temperature of 26 ºC and photoperiod of 16 h/8 h light/dark in every 24 h cycle showed best response for in vitro culturing of Lilium.

Keywords

Cytokinins Auxins Micropropagation Callogenesis Lilium
Ali, A., Yasmin, S., Shaheen Niazi, R., Majid, A., & Huma Naveed, N. (2012). Role of Different Cytokinins and Auxins for Micropropagation, Callogenesis and Plant Regeneration in Lilium (Lilium longiflorum). Asian Journal of Chemistry, 25(1), 427–432. https://doi.org/10.14233/ajchem.2013.13140
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