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Vol. 27 No. 9 (2015): Vol 27 Issue 9

Copyright (c) 2015 AJC

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Determination of Bovine Serum Albumin and Metronidazole by Electrochemical and Fluorescent Methods

https://doi.org/10.14233/ajchem.2015.18649
Gen Liu
School of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, P.R. China
Wanjun Wang
School of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, P.R. China
Wei Ma
School of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, P.R. China
Dengming Sun
School of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, P.R. China

Corresponding Author(s) : Gen Liu

liugen7084@126.com

Asian Journal of Chemistry, Vol. 27 No. 9 (2015): Vol 27 Issue 9

Abstract

Poly(L-tyrosine)-graphene modified glassy carbon electrode (PLT-GN/GCE) was prepared by cyclic voltammetric method. Electrochemical method, fluorescence and UV-visible spectroscopy were applied to study the interaction of metronidazole and bovine serum albumin. Fluorescence spectrum of bovine serum albumin in the presence of metronidazole clearly shows that metronidazole acts as a quencher and it is a static quenching process. From the experimental data of electrochemical and fluorescent experiment, the binding constants were deduced to be 5.481 × 104 and 5.369 × 104 L mol-1, respectively and the numbers of binding site were 1.422 and 1.468 at 25 °C, respectively. The calibration plots for bovine serum albumin with electrochemical method and for metronidazole with fluorescent method were in the range of 5 × 10-7 to 1 × 10-4 and 2 × 10-7 to 7 × 10-5 mol L-1, respectively and the detection limits for bovine serum albumin and metronidazole were 1 × 10-7 and 5 × 10-8 mol L-1, respectively. The method had been successfully applied to the determination of metronidazole and bovine serum albumin in samples with satisfactory results.

Keywords

Metronidazole Bovine serum albumin Graphene L-Tyrosine Determination
Liu, G., Wang, W., Ma, W., & Sun, D. (2015). Determination of Bovine Serum Albumin and Metronidazole by Electrochemical and Fluorescent Methods. Asian Journal of Chemistry, 27(9), 3333–3338. https://doi.org/10.14233/ajchem.2015.18649
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