Copyright (c) 2015 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Direct TLC Optical Resolution of Two Sulphur-Containing DL-a-Amino Acids Using Vancomycin as Chiral Selector
Corresponding Author(s) : Min Zi
Asian Journal of Chemistry,
Vol. 27 No. 9 (2015): Vol 27 Issue 9
Abstract
Direct TLC enantioseparation for naturally occurring two sulphur-containing a-amino acids, cysteine and methionine, were achieved on silica gel plates using vancomycin as chiral impregnating reagent with only one developing solvent n-butanol-methanol-water- acetic acid (5:1.6:1.2:0.4, v/v/v/v). The effects of developing solvents, the ratio of mobile phase, concentration of chiral selector and temperature on the enantioseparation had been studied. This is the first report on TLC direct resolution of cysteine and methionine using vancomycin as chiral selector with same developing solvent.
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- M. Del Bubba, L. Checchini and L. Lepri, Anal. Bioanal. Chem., 2, 533 (2013); doi:10.1007/s00216-012-6514-5.
- M. Sajewicz and T. Kowalska, Acta Chromatogr., 22, 499 (2010); doi:10.1556/AChrom.22.2010.4.1.
- R. Bhushan and J. Martens, Biomed. Chromatogr., 11, 280 (1997); doi:10.1002/(SICI)1099-0801(199709)11:5<280::AID-BMC697>3.0.CO;2-U.
- R. Bhushan and J. Martens, Biomed. Chromatogr., 15, 155 (2001); doi:10.1002/bmc.56.
- R. Bhushan and V. Parshad, J. Chromatogr. A, 736, 235 (1996); doi:10.1016/0021-9673(95)01330-X.
- R. Bhushan and G.T. Thiongo, J. Planar Chromatogr., 13, 33 (2000).
- L. Lepri, V. Coas and P.G. Desideri, J. Planar Chromatogr., 4, 338 (1991).
- R. Bhushan and M. Arora, Biomed. Chromatogr., 15, 433 (2001); doi:10.1002/bmc.96.
- R. Bhushan, J. Martens and G.T. Thiongo, J. Pharm. Biomed. Anal., 21, 1143 (2000); doi:10.1016/S0731-7085(99)00203-4.
- R. Bhushan, G.P. Reddy and S. Joshi, J. Planar Chromatogr., 7, 126 (1994).
- K. Günther, J. Martens and M. Schickedanz, Angew. Chem. Int. Ed. Engl., 23, 506 (1984); doi:10.1002/anie.198405061.
- C. Yuan, J. Planar Chromatogr., 27, 318 (2014); doi:10.1556/JPC.27.2014.4.13.
- C. Yuan, Asian J. Chem., 27, 2043 (2015); doi:10.14233/ajchem.2015.17824.
- D.W. Armstrong and Y. Zhou, J. Liq. Chromatogr., 17, 1695 (1994); doi:10.1080/10826079408013451.
- L. Lepri, V. Coas, P.G. Desideri and D. Santianni, Chromatographia, 36, 297 (1993); doi:10.1007/BF02263881.
- L. Lepri, V. Coas, P.G. Desideri and L. Pettini, J. Planar Chromatogr., 5, 364 (1992).
- A. Berthod, Y. Liu, C. Bagwill and D.W. Armstrong, J. Chromatogr. A, 731, 123 (1996); doi:10.1016/0021-9673(95)01198-6.
- D.W. Armstrong, Y. Tang, S. Chen, Y. Zhou, C. Bagwill and J.R. Chen, Anal. Chem., 66, 1473 (1994); doi:10.1021/ac00081a019.
- A. Péter, G. Török and D.W. Armstrong, J. Chromatogr. A, 793, 283 (1998); doi:10.1016/S0021-9673(97)00938-2.
- A. Péter, G. Török, D.W. Armstrong, G. Tóth and D. Tourwe, J. Chromatogr. A, 904, 1 (2000); doi:10.1016/S0021-9673(00)00917-1.
- G. Török, A. Péter, D.W. Armstrong, D. Tourwe, G. Tóth and J. Sapi, Chirality, 13, 648 (2001); doi:10.1002/chir.10004.
References
M. Del Bubba, L. Checchini and L. Lepri, Anal. Bioanal. Chem., 2, 533 (2013); doi:10.1007/s00216-012-6514-5.
M. Sajewicz and T. Kowalska, Acta Chromatogr., 22, 499 (2010); doi:10.1556/AChrom.22.2010.4.1.
R. Bhushan and J. Martens, Biomed. Chromatogr., 11, 280 (1997); doi:10.1002/(SICI)1099-0801(199709)11:5<280::AID-BMC697>3.0.CO;2-U.
R. Bhushan and J. Martens, Biomed. Chromatogr., 15, 155 (2001); doi:10.1002/bmc.56.
R. Bhushan and V. Parshad, J. Chromatogr. A, 736, 235 (1996); doi:10.1016/0021-9673(95)01330-X.
R. Bhushan and G.T. Thiongo, J. Planar Chromatogr., 13, 33 (2000).
L. Lepri, V. Coas and P.G. Desideri, J. Planar Chromatogr., 4, 338 (1991).
R. Bhushan and M. Arora, Biomed. Chromatogr., 15, 433 (2001); doi:10.1002/bmc.96.
R. Bhushan, J. Martens and G.T. Thiongo, J. Pharm. Biomed. Anal., 21, 1143 (2000); doi:10.1016/S0731-7085(99)00203-4.
R. Bhushan, G.P. Reddy and S. Joshi, J. Planar Chromatogr., 7, 126 (1994).
K. Günther, J. Martens and M. Schickedanz, Angew. Chem. Int. Ed. Engl., 23, 506 (1984); doi:10.1002/anie.198405061.
C. Yuan, J. Planar Chromatogr., 27, 318 (2014); doi:10.1556/JPC.27.2014.4.13.
C. Yuan, Asian J. Chem., 27, 2043 (2015); doi:10.14233/ajchem.2015.17824.
D.W. Armstrong and Y. Zhou, J. Liq. Chromatogr., 17, 1695 (1994); doi:10.1080/10826079408013451.
L. Lepri, V. Coas, P.G. Desideri and D. Santianni, Chromatographia, 36, 297 (1993); doi:10.1007/BF02263881.
L. Lepri, V. Coas, P.G. Desideri and L. Pettini, J. Planar Chromatogr., 5, 364 (1992).
A. Berthod, Y. Liu, C. Bagwill and D.W. Armstrong, J. Chromatogr. A, 731, 123 (1996); doi:10.1016/0021-9673(95)01198-6.
D.W. Armstrong, Y. Tang, S. Chen, Y. Zhou, C. Bagwill and J.R. Chen, Anal. Chem., 66, 1473 (1994); doi:10.1021/ac00081a019.
A. Péter, G. Török and D.W. Armstrong, J. Chromatogr. A, 793, 283 (1998); doi:10.1016/S0021-9673(97)00938-2.
A. Péter, G. Török, D.W. Armstrong, G. Tóth and D. Tourwe, J. Chromatogr. A, 904, 1 (2000); doi:10.1016/S0021-9673(00)00917-1.
G. Török, A. Péter, D.W. Armstrong, D. Tourwe, G. Tóth and J. Sapi, Chirality, 13, 648 (2001); doi:10.1002/chir.10004.