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Vol. 27 No. 9 (2015): Vol 27 Issue 9

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Effect of Extraction Conditions on Purity and Structure of Pectin in Tobacco by CP/MAS 13C NMR Spectroscopy

https://doi.org/10.14233/ajchem.2015.18799
Xiao-Lan Zhu
Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China
Yusong Wang
Lab for Physical and Chemistry of Analysis, University of Science and Technology of China, Hefei 230052, P.R. China
Yun Gao
Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China
Hui Lin
Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China
Jun Chen
Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China

Corresponding Author(s) : Xiao-Lan Zhu

zxl8906@ustc.edu.cn

Asian Journal of Chemistry, Vol. 27 No. 9 (2015): Vol 27 Issue 9

Abstract

The structure and composition of pectins depend on their source, the method used to extract from other soluble material, the analytical procedures and so on. This study was to investigate the influence of extraction conditions (pH, temperature and extraction time) on the yield, purity and structure of pectin in tobacco by CP/MAS 13C NMR spectroscopy. The results showed that the highest yield of pectin (10.87 %) was obtained at pH 1.5, but the highest purity of pectin (galacturonic acid content, GalA) was obtained at pH 2, which was 78.4 %. The degrees of methylation and acetylation decreased with increasing of acid strength (at pH 1.5-2.5), temperature and extraction time. With stronger acid, higher temperature and longer time, the degrees of methylation and degrees of acetylation of pectin decrease. These data provides a prerequisite for controlling and manipulating pectins processing properties.

Keywords

Pectin Extraction conditions Purity Structure CP/MAS 13C NMR
Zhu, X.-L., Wang, Y., Gao, Y., Lin, H., & Chen, J. (2015). Effect of Extraction Conditions on Purity and Structure of Pectin in Tobacco by CP/MAS 13C NMR Spectroscopy. Asian Journal of Chemistry, 27(9), 3365–3369. https://doi.org/10.14233/ajchem.2015.18799
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