A New Method for Estimation of Surfactin

Muhammad Usman Mubarak; Ahmad Adnan; Muhammad Imtiaz Shafiq; Abdul Rehman; Javeed A. Awan

doi:10.14233/ajchem.2016.19367

Issue

Vol. 28 No. 1 (2016): Vol 28 Issue 1

Issue Published : October 5, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

A New Method for Estimation of Surfactin

https://doi.org/10.14233/ajchem.2016.19367

Muhammad Usman Mubarak

Institute of Chemistry, University of the Punjab, Lahore-54000, Pakistan; Government College University, Lahore-54000, Pakistan

Ahmad Adnan

Government College University, Lahore-54000, Pakistan

Muhammad Imtiaz Shafiq

Institute of Chemistry, University of the Punjab, Lahore-54000, Pakistan; Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore-54000, Pakistan

Abdul Rehman

Institute of Chemical Engineering and Technology University of the Punjab, Lahore-54000, Pakistan

Javeed A. Awan

Institute of Chemical Engineering and Technology University of the Punjab, Lahore-54000, Pakistan

Corresponding Author(s) : Muhammad Imtiaz Shafiq

imtiazshafiq@gmail.com

Asian Journal of Chemistry, Vol. 28 No. 1 (2016): Vol 28 Issue 1
Article Published : October 6, 2015

Abstract

Surfactin is a lipopeptide biosurfactant produced by some strains of Bacillus subtilis. Due to its surface tension reducing ability it has a potential application as a drug in certain types of cancer diseases. It has a strong ability to cause hemolysis. In this work the hemolytic activity of surfactin was used for its estimation by measuring released haemoglobin spectrophotometrically. Herein a new method has been developed involving microscopic counting of red blood cells for surfactin estimation. There has been a good agreement between the newly developed microscopic counting of red blood cells and already reported spectrophotometric method.

Keywords

Surfactin Bacillus subtilis Red blood cells Haemoglobin Hemolytic activity

Mubarak, M. U., Adnan, A., Shafiq, M. I., Rehman, A., & Awan, J. A. (2015). A New Method for Estimation of Surfactin. Asian Journal of Chemistry, 28(1), 230–232. https://doi.org/10.14233/ajchem.2016.19367

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References

K. Arima, A. Kakinuma and G. Tamura, Biochem. Biophys. Res. Commun., 31, 488 (1968); doi:10.1016/0006-291X(68)90503-2.
M.S. Yeh, Y.H. Wei and J.S. Chang, Biotechnol. Prog., 21, 1329 (2005); doi:10.1021/bp050040c.
S.-Y. Kim, J.Y. Kim, S.-H. Kim, H.J. Bae, H. Yi, S.H. Yoon, B.S. Koo, M. Kwon, J.Y. Cho, C.E. Lee and S. Hong, FEBS Lett., 581, 865 (2007); doi:10.1016/j.febslet.2007.01.059.
X. Cao, A. Wang, C. Wang, D. Mao, M. Lu, Y. Cui and R. Jiao, Chem. Biol. Interact., 183, 357 (2010); doi:10.1016/j.cbi.2009.11.027.
A. Grau, J.C. Gómez Fernández, F. Peypoux and A. Ortiz, Biomembranes, 1418, 307 (1999); doi:10.1016/S0005-2736(99)00039-5.
D. Gerson and J. Zajic, Dev. Ind. Microbiol., 19, 577 (1978).
D.A. Davis, H.C. Lynch and J. Varley, Enzyme Microb. Technol., 25, 322 (1999); doi:10.1016/S0141-0229(99)00048-4.
R.S. Makkar and S.S. Cameotra, J. Ind. Microbiol. Biotechnol., 20, 48 (1998); doi:10.1038/sj.jim.2900474.
M.P. Vinardell and M.R. Infante, Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol., 124, 117 (1999); doi:10.1016/S0742-8413(99)00057-2.
I.M. Banat, Biotechnol. Lett., 15, 591 (1993); doi:10.1007/BF00138546.
M. Kracht, H. Rokos, M. Ozel, M. Kowall, G. Pauli and J. Vater, J. Antibiot., 52, 613 (1999); doi:10.7164/antibiotics.52.613.
F. Peypoux, J. Bonmatin and J. Wallach, Appl. Microbiol. Biotechnol., 51, 553 (1999); doi:10.1007/s002530051432.
A. Morán, M.A. Martínez and F. Siñeriz, Biotechnol. Lett., 24, 177 (2002); doi:10.1023/A:1014140820207.
S.-C. Lin and H.-J. Jiang, Biotechnol. Techniq., 11, 413 (1997); doi:10.1023/A:1018468723132.
F. Mottu, M.-J. Stelling, D.A. Rüfenacht and E. Doelker, PDA J. Pharm. Sci. Technol., 55, 16 (2001).

References

K. Arima, A. Kakinuma and G. Tamura, Biochem. Biophys. Res. Commun., 31, 488 (1968); doi:10.1016/0006-291X(68)90503-2.

M.S. Yeh, Y.H. Wei and J.S. Chang, Biotechnol. Prog., 21, 1329 (2005); doi:10.1021/bp050040c.

S.-Y. Kim, J.Y. Kim, S.-H. Kim, H.J. Bae, H. Yi, S.H. Yoon, B.S. Koo, M. Kwon, J.Y. Cho, C.E. Lee and S. Hong, FEBS Lett., 581, 865 (2007); doi:10.1016/j.febslet.2007.01.059.

X. Cao, A. Wang, C. Wang, D. Mao, M. Lu, Y. Cui and R. Jiao, Chem. Biol. Interact., 183, 357 (2010); doi:10.1016/j.cbi.2009.11.027.

A. Grau, J.C. Gómez Fernández, F. Peypoux and A. Ortiz, Biomembranes, 1418, 307 (1999); doi:10.1016/S0005-2736(99)00039-5.

D. Gerson and J. Zajic, Dev. Ind. Microbiol., 19, 577 (1978).

D.A. Davis, H.C. Lynch and J. Varley, Enzyme Microb. Technol., 25, 322 (1999); doi:10.1016/S0141-0229(99)00048-4.

R.S. Makkar and S.S. Cameotra, J. Ind. Microbiol. Biotechnol., 20, 48 (1998); doi:10.1038/sj.jim.2900474.

M.P. Vinardell and M.R. Infante, Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol., 124, 117 (1999); doi:10.1016/S0742-8413(99)00057-2.

I.M. Banat, Biotechnol. Lett., 15, 591 (1993); doi:10.1007/BF00138546.

M. Kracht, H. Rokos, M. Ozel, M. Kowall, G. Pauli and J. Vater, J. Antibiot., 52, 613 (1999); doi:10.7164/antibiotics.52.613.

F. Peypoux, J. Bonmatin and J. Wallach, Appl. Microbiol. Biotechnol., 51, 553 (1999); doi:10.1007/s002530051432.

A. Morán, M.A. Martínez and F. Siñeriz, Biotechnol. Lett., 24, 177 (2002); doi:10.1023/A:1014140820207.

S.-C. Lin and H.-J. Jiang, Biotechnol. Techniq., 11, 413 (1997); doi:10.1023/A:1018468723132.

F. Mottu, M.-J. Stelling, D.A. Rüfenacht and E. Doelker, PDA J. Pharm. Sci. Technol., 55, 16 (2001).

Issue

Vol. 28 No. 1 (2016): Vol 28 Issue 1

A New Method for Estimation of Surfactin

Corresponding Author(s) : Muhammad Imtiaz Shafiq

Abstract

Keywords

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