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Vol. 28 No. 1 (2016): Vol 28 Issue 1

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Effect of Cross-Linkers on Activity of Alcohol Dehydrogenase Immobilized on Polyaniline Magnetic Nanoparticles

https://doi.org/10.14233/ajchem.2016.19229
Lihua Jin
Colloge of Bioengineering, Beijing Polytechnic, Beijing 100029, P.R. China
Ye Li
Colloge of Bioengineering, Beijing Polytechnic, Beijing 100029, P.R. China
Xiang Hao Ren
Key Laboratory of Urban Stormwater System and Water Environment, Ministry of Education, School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing 100044, P.R. China

Corresponding Author(s) : Lihua Jin

kimlihua@qq.com

Asian Journal of Chemistry, Vol. 28 No. 1 (2016): Vol 28 Issue 1

Abstract

In this study, alcohol dehdyrogenase (ADH) was immobilized on polyaniline magnetic nanoparticles (PAMP), which were used to completely recover the immobilized enzyme. When glutaraldehyde was used as a cross linker to immobilize alcohol dehdyrogenase on polyaniline magnetic nanoparticles, no alcohol dehdyrogenase activity was observed after immobilization. However, when 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) was used as a cross linker, immobilized alcohol dehdyrogenase was found to display improved activity. The Kcat values of the free and immobilized alcohol dehdyrogenases were determined to be 82 and 17 mM/min/mg protein, respectively. Immobilized alcohol dehdyrogenase showed enhanced stability under acidic conditions at pH 4.5. While the activity of free alcohol dehdyrogenase was zero, the activity of immobilized alcohol dehdyrogenase was 50 % of its original activity at pH 4.5. The immobilized enzyme was also highly stable under rigorous shaking conditions relative to free alcohol dehdyrogenase. After 30 days of incubation at room temperature and 200 rpm, the residual activity of the free enzyme was zero, whereas that of the immobilized alcohol dehdyrogenase was 90.7 % after 60 days. The immobilized alcohol dehdyrogenase was recovered after completion of the enzyme reaction and it was still active after 15 consecutive reactions. The activity of immobilized alcohol dehdyrogenase was over 90 % of its original activity.

Keywords

Alcohol dehydrogenase Glutaraldehyde Polyaniline magnetic nanoparticles
Jin, L., Li, Y., & Ren, X. H. (2015). Effect of Cross-Linkers on Activity of Alcohol Dehydrogenase Immobilized on Polyaniline Magnetic Nanoparticles. Asian Journal of Chemistry, 28(1), 55–59. https://doi.org/10.14233/ajchem.2016.19229
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References
  1. S.M. Thomas, R. DiCosimo and V. Nagarajan, Trends Biotechnol., 20, 238 (2002); doi:10.1016/S0167-7799(02)01935-2.
  2. G. Cea, L. Wilson, J.M. Bolívar, A. Markovits and A. Illanes, Enzyme Microb. Technol., 44, 135 (2009); doi:10.1016/j.enzmictec.2008.10.021.
  3. S.J. Benkovic and A. Ballesteros, Trends Biotechnol., 15, 385 (1997); doi:10.1016/S0167-7799(97)01098-6.
  4. H. Zhao, K. Chockalingam and Z. Chen, Curr. Opin. Biotechnol., 13, 104 (2002); doi:10.1016/S0958-1669(02)00291-4.
  5. J.N. Talbert and J.M. Goddard, Food Bioprod. Process., 91, 693 (2013); doi:10.1016/j.fbp.2013.08.006.
  6. J.N. Talbert and J.M. Goddard, Process Biochem., 48, 656 (2013); doi:10.1016/j.procbio.2013.03.001.
  7. K. Pospiskova and I. Safarik, Carbohydr. Polym., 96, 545 (2013); doi:10.1016/j.carbpol.2013.04.014.
  8. G. Lee, J. Kim and J.- Lee, Enzyme Microb. Technol., 42, 466 (2008); doi:10.1016/j.enzmictec.2007.12.006.
  9. G. Lee, H. Joo and J.- Lee, J. Mol. Catal., B Enzym., 54, 116 (2008); doi:10.1016/j.molcatb.2007.11.020.
  10. K.E. Lee, M. Wang, E.J. Kim and S.H. Hahn, Curr. Appl. Phys., 9, 683 (2009); doi:10.1016/j.cap.2008.06.006.
  11. N.-J. Kim, S.- Choi, H.J. Lee and K.J. Kim, Curr. Appl. Phys., 9, 643 (2009); doi:10.1016/j.cap.2008.04.015.
  12. K. Yu, T. Luo, Y. Zhang, C. Yang, L. Shang, C. Li and Q. Li, Mater. Lett., 63, 566 (2009); doi:10.1016/j.matlet.2008.11.045.
  13. S. Park, S.H. Kim, K. Won, J.W. Choi, Y.H. Kim, H.J. Kim, Y.-H. Yang and S.H. Lee, Carbohydr. Polym., 115, 223 (2015); doi:10.1016/j.carbpol.2014.08.096.
  14. P. Jochems, T. Mueller, Y. Satyawali, L. Diels, W. Dejonghe and U. Hanefeld, Biochem. Eng. J., 93, 137 (2015); doi:10.1016/j.bej.2014.10.007.
  15. B.R. Facin, B. Moret, D. Baretta, L.A. Belfiore and A.T. Paulino, Food Chem., 179, 44 (2015); doi:10.1016/j.foodchem.2015.01.088.
  16. G.Y. Li, K.L. Huang, Y.R. Jiang, D.L. Yang and P. Ding, Int. J. Biol. Macromol., 42, 405 (2008); doi:10.1016/j.ijbiomac.2008.01.005.
  17. M.C.C. Buc, Enzyme Technology, Cambridge University Press, Cambridge (1990).
  18. S. Park, Y. Lee, D.N. Kim, S. Park, E. Jang and W.-G. Koh, React. Funct. Polym., 69, 293 (2009); doi:10.1016/j.reactfunctpolym.2009.02.001.
  19. M. Matto and Q. Husain, J. Mol. Catal., B Enzym., 57, 164 (2009); doi:10.1016/j.molcatb.2008.08.011.
  20. M.P. Massafera and S.I.C. Torresi, Sens. Actuators B Chem., 137, 476 (2009); doi:10.1016/j.snb.2009.02.013.
  21. D.E. Wong, M. Dai, J.N. Talbert, S.R. Nugen and J.M. Goddard, J. Mol. Catal., B Enzym., 110, 16 (2014); doi:10.1016/j.molcatb.2014.09.007.
  22. I. Van de Voorde, K. Goiris, E. Syryn, C. Van den Bussche and G. Aerts, Process Biochem., 49, 2134 (2014); doi:10.1016/j.procbio.2014.09.010.
  23. S.K.S. Patel, V.C. Kalia, J.-H. Choi, J.-R. Haw, I.-W. Kim and J.K. Lee, J. Microbiol. Biotechnol., 24, 639 (2014); doi:10.4014/jmb.1401.01025.
  24. J. Kim, J.W. Grate and P. Wang, Chem. Eng. Sci., 61, 1017 (2006); doi:10.1016/j.ces.2005.05.067.
  25. T. Godjevargova, R. Nenkova and V. Konsulov, J. Mol. Catal., B Enzym., 38, 59 (2006); doi:10.1016/j.molcatb.2005.11.010.
  26. K. Gabrovska, A. Georgieva, T. Godjevargova, O. Stoilova and N. Manolova, J. Biotechnol., 129, 674 (2007); doi:10.1016/j.jbiotec.2007.01.014.
  27. T. Godjevargova and K. Gabrovska, J. Biotechnol., 103, 107 (2003); doi:10.1016/S0168-1656(03)00107-X.
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