Promotion Effect of La3+ on the Catalytic Activity of Horseradish Peroxidase in vitro

Shun Wang; Shaofen Guo; Guangna Zhang; Lu Cao; Zhonghe Tian

doi:10.14233/ajchem.2015.17832

Issue

Vol. 27 No. 7 (2015): Vol 27 Issue 7, 2015

Issue Published : March 29, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Promotion Effect of La3+ on the Catalytic Activity of Horseradish Peroxidase in vitro

https://doi.org/10.14233/ajchem.2015.17832

Shun Wang

College of Resources and Environment, Linyi University, Linyi 276005, P.R. China

Shaofen Guo

Shandong Provincial Key Laboratory of Water and Soil Conservation & Environmental Protection, Linyi University, Linyi 276005, P.R. China; College of Resources and Environment, Linyi University, Linyi 276005, P.R. China

Guangna Zhang

Shandong Provincial Key Laboratory of Water and Soil Conservation & Environmental Protection, Linyi University, Linyi 276005, P.R. China

Lu Cao

College of Resources and Environment, Linyi University, Linyi 276005, P.R. China

Zhonghe Tian

College of Resources and Environment, Northeast Agricultural University, Harbin 150030, P.R. China

Corresponding Author(s) : Shaofen Guo

guoshaofen@lyu.edu.cn

Asian Journal of Chemistry, Vol. 27 No. 7 (2015): Vol 27 Issue 7, 2015
Article Published : March 29, 2015

Abstract

In this paper, the promotion effect of La³⁺ on the catalytic activity of horseradish peroxidase was investigated in vitro by using the combination of cyclic voltammetry, ultraviolet-visible, atomic force microscopy, high performance liquid chromatography and inductively coupled plasma mass spectrometry. The results indicated that the low concentration of La³⁺ could promote the catalytic activity of horseradish peroxidase by changing the secondary structure or the conformation of horseradish peroxidase and loosing the tertiary structure of the horseradish peroxidase and/or acting as an activator. La³⁺ could lead to the decrease in planarity of the porphyrin cycle in heme group, thus the exposure extent of heme Fe(III) is increased, the electron transfer is convenient and thus horseradish peroxidase catalytic activity is promoted, but La³⁺ did not bind with horseradish peroxidase. Therefore, there exists a weak interaction between La³⁺ and horseradish peroxidase in vitro. The result could provide a reference point on better understanding in agricultural application of rare earth elements.

Keywords

La3 Horseradish peroxidase Catalytic activity Conformation change

Wang, S., Guo, S., Zhang, G., Cao, L., & Tian, Z. (2015). Promotion Effect of La3+ on the Catalytic Activity of Horseradish Peroxidase in vitro. Asian Journal of Chemistry, 27(7), 2365–2368. https://doi.org/10.14233/ajchem.2015.17832

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References

H. Liu, P. He, Z. Li, Y. Liu and J. Li, Electrochim. Acta, 51, 1925 (2006); doi:10.1016/j.electacta.2005.06.034.

Y.-W. He and C.-S. Loh, Plant Sci., 159, 117 (2000); doi:10.1016/S0168-9452(00)00338-1.

H. Wu, X. Zhang, P. Liao, Z. Li, W. Li, X. Li, Y. Wu and F. Pei, J. Inorg. Biochem., 99, 2151 (2005); doi:10.1016/j.jinorgbio.2005.07.014.

J.Z. Ni, Bioinorganic Chemistry of Rare-Earth Elements, Science Press, Beijing, pp. 18 (1995). (in Chinese).

C. Liang, X. Huang, W. Tao and Q. Zhou, J. Rare Earths, 24, 364 (2006); doi:10.1016/S1002-0721(06)60125-7.

S.F. Guo, R. Cao, A.H. Lu, Q. Zhou, T.H. Lu, X.L. Ding, C.J. Li and X.H. Huang, J. Biol. Inorg. Chem., 13, 587 (2008); doi:10.1007/s00775-008-0347-x.

S.F. Guo, Q. Zhou, T.H. Lu, X.L. Ding and X.H. Huang, Spectrochim. Acta A, 70, 818 (2008); doi:10.1016/j.saa.2007.09.039.

K. Chattopadhyay and S. Mazumdar, Biochemistry, 39, 263 (2000); doi:10.1021/bi990729o.

D. Pahari, A. Patel and D. Behere, J. Inorg. Biochem., 60, 245 (1995); doi:10.1016/0162-0134(95)00024-0.

S.F. Guo, L.H. Wang, A.H. Lu, T.H. Lu, X.L. Ding and X.H. Huang, Spectrochim. Acta A, 75, 936 (2010); doi:10.1016/j.saa.2009.11.033.

K. Wang, R. Li, Y. Cheng and B. Zhu, Coord. Chem. Rev., 190-192, 297 (1999); doi:10.1016/S0010-8545(99)00072-7.

J.L. Tang, J.G. Jiang, Y.H. Song, Z.Q. Peng, Z.Y. Wu, S.J. Dong and E.K. Wang, Chem. Phys. Lipids, 120, 119 (2002); doi:10.1016/S0009-3084(02)00109-3.

Y. Zhang, P. He and N. Hu, Electrochim. Acta, 49, 1981 (2004); doi:10.1016/j.electacta.2003.12.028.

D. Sun, C. Cai, X. Li, W. Xing and T. Lu, J. Electroanal. Chem., 566, 415 (2004); doi:10.1016/j.jelechem.2003.11.055.

H.J. Jiang, X.H. Huang, X.F. Wang, X. Li, W. Xing, X.L. Ding and T.H. Lu, J. Electroanal. Chem., 545, 83 (2003); doi:10.1016/S0022-0728(03)00111-6.

Issue

Vol. 27 No. 7 (2015): Vol 27 Issue 7, 2015

Promotion Effect of La3+ on the Catalytic Activity of Horseradish Peroxidase in vitro

Corresponding Author(s) : Shaofen Guo

Abstract

Keywords

Full Article

Download Citation

References

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