Issue

Vol. 28 No. 1 (2016): Vol 28 Issue 1

Copyright (c) 2016 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Content of Arabinogalactan from Cherry Gum (Prunus avium) and as Polymer Carrier for Immobilization of Trypsin

https://doi.org/10.14233/ajchem.2016.19318
Shamo Z. Tapdigov
Nanostructured Metal-Polymer Catalysis Department, Institute of Catalysis and Inorganic Chemistry named after Academician M. Nagiyev, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Nizami A. Zeynalov
Nanostructured Metal-Polymer Catalysis Department, Institute of Catalysis and Inorganic Chemistry named after Academician M. Nagiyev, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Saadat F. Humbatova
Nanostructured Metal-Polymer Catalysis Department, Institute of Catalysis and Inorganic Chemistry named after Academician M. Nagiyev, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Jalal A. Nagiev
Department of Radiochemistry, Institute of Radiation Problems, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Aygun F. Isazadeh
Nanostructured Metal-Polymer Catalysis Department, Institute of Catalysis and Inorganic Chemistry named after Academician M. Nagiyev, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Mirvari Kh. Hasanova
Nanostructured Metal-Polymer Catalysis Department, Institute of Catalysis and Inorganic Chemistry named after Academician M. Nagiyev, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Samira F. Safaraliyeva
Nanostructured Metal-Polymer Catalysis Department, Institute of Catalysis and Inorganic Chemistry named after Academician M. Nagiyev, Azerbaijan National Academy of Sciences, Baku, Azerbaijan

Corresponding Author(s) : Shamo Z. Tapdigov

shamotapdiqov@mail.ru

Asian Journal of Chemistry, Vol. 28 No. 1 (2016): Vol 28 Issue 1

Abstract

In this research work, we studied the content, structure and properties of arabinogalactan separated from cherry gum. The main polymer chain and linked polysaccharide macromolecules were investigated by NMR and FTIR spectroscopy. We also defined the amount of some metals ion (Na, Mg, Ca, K, Zn, Cu, Mn, Fe, Pb, Cd) and activity as 137Cs, 226Ra, 228Ra, 40K, 235U, 238U radioactive elements in content by atom-absorption spectroscopy and g-spectrometer. Hydrogels based on arabinogalactan were synthesized and immobilization of trypsin was studied. It was shown that at pH = 8 during the first 3 h separation of trypsin into solution is 8 %, but after 40 h it is found to be 92-98 %.

Keywords

Arabinogalactan Polysaccharide Cherry gum NMR and FTIR spectroscopy Immobilization Trypsin
Tapdigov, S. Z., Zeynalov, N. A., Humbatova, S. F., Nagiev, J. A., Isazadeh, A. F., Hasanova, M. K., & Safaraliyeva, S. F. (2015). Content of Arabinogalactan from Cherry Gum (Prunus avium) and as Polymer Carrier for Immobilization of Trypsin. Asian Journal of Chemistry, 28(1), 189–193. https://doi.org/10.14233/ajchem.2016.19318
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. N.A. Peppas, Crit. Opin. Colloid Interface Sci., 2, 531 (1997); doi:10.1016/S1359-0294(97)80103-3.
  2. E.V. Groman, E.T. Menz, P.M. Enriquez and C. Jung, Delivery of Therapeutic Agents to Receptors using Polysaccharides, US Patent, 5,544,386 (1996).
  3. J. Kost and R. Langer, in ed. N.A. Peppas, Hydrogels in Medicine and Pharmacy, CRC Press Inc. Boca Raton, vol. 3, p. 95 (1987).
  4. J. Liu, L. Li and Y. Cai, Eur. Polym. J., 42, 1767 (2006); doi:10.1016/j.eurpolymj.2006.03.005.
  5. K.M. Manjanna, Pharm. Res., 2, 1271 (2009).
  6. G. Fariba and V.F. Ebrahim, Iranian Polymer J., 18, 63 (2009).
  7. P.L. Brannon, Med. Plastics Biomater., 4, 34 (1997).
  8. E.H. Schacht, J. Phys. Conf. Ser., 3, 22 (2004); doi:10.1088/1742-6596/3/1/004.
  9. T. Coviello, P. Matricardi, C. Marianecci and F. Alhaique, J. Control. Rel., 119, 5 (2007); doi:10.1016/j.jconrel.2007.01.004.
  10. X. Chen, W. Li, W. Zhong, Y. Lu and T. Yu, J. Appl. Polym. Sci., 65, 2257 (1997); doi:10.1002/(SICI)1097-4628(19970912)65:11<2257::AID-APP23>3.0.CO;2-Z.
  11. H. Hara, M. Anezaki and K. Sciba, Rice Cake Products Containing Polysaccharides Storable at Ambient Temperature, US Patent 2000197457 A2, Chem. Abstr., 133, 104231 (2000).
  12. K.A. Smeds, A. Pfister-Serres, D.L. Hatchella and M.W. Grinstaff, J. Macromol. Sci. Part A, 36, 981 (1999); doi:10.1080/10601329908951194.
  13. D.S. Magnin, E.C. Dumitriu and E. Chornet, J. Bioact. Compat. Polym., 18, 355 (2003); doi:10.1177/0883911503038375.
  14. M. Prabaharan and J.F. Mano, Macromol. Biosci., 6, 991 (2006); doi:10.1002/mabi.200600164.
  15. A.R. Manrich, C.M. Galvão, C.D. Jesus, R.C. Giordano and R.L.C. Giordano, J. Biol. Macrom., 43, 54 (2008); doi:10.1016/j.ijbiomac.2007.11.007.
  16. A.P. Nikolas, Biomaterials Sci., 12, 35 (2003).
  17. M.G. Cascone, N. Barbani, C.C. P.Giusti, G. Ciardelli and L. Lazzeri, J. Biomater. Sci., 12, 267 (2001); doi:10.1163/156856201750180807.
  18. A.Ya. Chernyak, K.V. Antonov, B.A. Dmitriey, N.K. Kochetkov, L.N. Padyukov and N.V. Tsvetkova, Russain J. Bioorg. Chem., 10, 1376 (1984).
  19. L.A. Badykova, Ph.D. Thesis, Interaction of the Siberian Larch Arabinogalactan with 5-Aminosalicylic acid, 4-Aminosalycilic acid and Hydrazide, Ufa, Russia, p. 27 (2007).
  20. M.R. Mucalo, C.R. Bullen, M. Manley-Harris and T.M. McIntire, J. Mater. Sci., 37, 493 (2002); doi:10.1023/A:1013757221776.
  21. L.A. Grishchenko, G.P. Aleksandrova, L.P. Feoktistova, A.N. Sapozhnikov, B.G. Sukhov and B.A. Trofimov, Russian J. Gen. Chem., 76, 1111 (2006); doi:10.1134/S1070363206070189.
  22. R.R. Robinson, J. Feirtag and J.L. Slavin, J. Am. Coll. Nutr., 20, 279 (2001); doi:10.1080/07315724.2001.10719048.
  23. R.V. Nunes, H.R. Mehansho, and R.I. Mellican, Beverage Compositions Comprising Arabinogalactan and Defined Vitamins, PCT In Appl. WO 2002026055 A2. 136 (2002).
  24. G.O. Aspinall, Some Recent Developments in the Chemistry of Arabinogalactans, Actes du Symposium International de Grenoble, pp. 89-97 (1964).
  25. S.A. Medvedeva, G.P. Aleksandrova, V.I. Dubrovina, T.D. Tchetverikova, L.A. Grishchenko, I.M. Krasnikova, L.P. Feoktictova and N.A. Tukavkina, Chem. Computat. Simul. Butlerov Commun., 7, 45 (2002).
  26. S. Willfor, R. Sjoholm and B. Holmbom, Isolation and Characterisation of Water-Soluble Arabinogalactans from Heartwood of Norway Spruce and Scots Pine, 10th International Symposium on Wood Pulping Chemistry Proceeding, Japan Tappi, Vol. 2, pp. 32-43 (1999).
  27. E.V. Groman, P.M. Enriquez, C. Jung and L. Josephson, Bioconjug. Chem., 5, 547 (1994); doi:10.1021/bc00030a010.
  28. J.H. Prescott, E.V. Groman and G. Gulyas, Carbohydr. Res., 301, 89 (1997); doi:10.1016/S0008-6215(97)00078-5.
  29. A.M. Showalter, Cell. Mol. Life Sci., 58, 1399 (2001); doi:10.1007/PL00000784.
  30. H. Yamada, Bioactive Arabinogalactan-Proteins and Related Pectic Polysaccharides in Sino-Japanese Herbal Medicines, Cell Dev. Biol. Arabinogalactan-Proteins, 20th Symposium Plant Physiological, Kluwer Academic/Plenum Publishers: New York, vol. 135, pp. 221-229 (2000).
  31. A.V. Reis, M.R. Guilherme, O.A. Cavalcanti, A.F. Rubira and E.C. Muniz, Polymer, 47, 2023 (2006); doi:10.1016/j.polymer.2006.01.058.
  32. A. Belicova, L. Ebringer, J. Krajcovic, Z. Hromádková and A. Ebringerová, World J. Microbiol. Biotechnol., 17, 293 (2001); doi:10.1023/A:1016676401954.
  33. A.E. Clarke, R.L. Anderson and B.A. Stone, Phytochemistry, 8, 521 (1979); doi:10.1016/S0031-9422(00)84255-7.
  34. O.A. Bushneva, R.Q. Ovodova, A.Ch. Shashcov and A.O. Chijov, Biochimiya, 71, 798 (2006).
  35. S. Willfor, R. Sjoholm, C. Laine and B. Holmbom, Wood Sci. Technol., 36, 101 (2002); doi:10.1007/s00226-001-0137-x.
  36. J. Wu, M. Luan and J. Zhao, Int. J. Biol. Macromol., 39, 185 (2006); doi:10.1016/j.ijbiomac.2006.03.017.
  37. R. Villalonga, A. Fragoso, R. Cao, P.D. Ortiz, M.L. Villalonga and A.E. Damiao, Supramol. Chem., 17, 387 (2005); doi:10.1080/10610270500126743.
  38. N.E. Kotelnikova, S.A. Mikhailova and E.N. Vlasova, Russian J. Appl. Chem., 80, 322 (2007); doi:10.1134/S1070427207020309.
  39. V.V. Korshak and M.I. Shitilman, Polymers in Processes Immobilization and Modification of Nature Compounds, Nauka, Moscow, pp. 260 (1984).
  40. L. Brannon-Peppas and N.A. Peppas, J. Control. Release, 8, 267 (1989); doi:10.1016/0168-3659(89)90048-5.
  41. E.A. Markvicheva, A.S. Bronin, N.E. Kudryavtseva, I.F. Kuzkina, I.I. Pashkin, Yu.E. Kirsh, L. D. Rumsh and V.P. Zubov, Russian J. Bioorg. Chem., 20, 257 (1994).
  42. L. Ding, Y. Li, Y. Jiang, Z. Cao and J. Huang, J. Polym. Sci., 8, 343 (2000).
  43. L.I. Valuev, T.A. Valueva and N.A. Plate, Uspexi biologicheskaya khim., 43, 30 (2003).
  44. I.Yu Galaev, Russian Chem. Rev., 64, 471 (1995); doi:10.1070/RC1995v064n05ABEH000161.
  45. V.P. Torchilin, I.L. Reyzer, V.H. Smirnov and E.I. Chazov, Russian J. Bioorg. Chem., 2, 1252 (1976).
  46. F.M. Sidelkovskaya, Chemistry N-vinylpyrrolidone and its Copolymers, Nauka, Moscow, p. 149 (1970).
  47. Yu.E. Kirsh, Poly-N-vinypyrrolidone and other Poly-N-vinylamide, Nauka, Moscow, p. 252 (1998).
  48. S.M. Mammedova, Sh.Z. Tapdiqov, S.F. Humbatova and N.A. Zeynalov, J. Chem. Chemical Eng., 8, 800 (2014).
  49. L.I. Valuev and N.A. Plate, Russian Polym. Sci., 34, 88 (1992).
  50. M. Nouaimi, K. Möschel and H. Bisswanger, J. Enzyme Microbiol., 29, 567 (2001); doi:10.1016/S0141-0229(01)00429-X.
  51. S.Z. Tapdiqov, S.R. Humbatova and N.A. Zeynalov, J. News Baku State Univ., 2, 22 (2013).
  52. A. Autissier and D.C. Letourneur, Eur. Cell. Mater., 3, 50 (2007).
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0