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Vol. 27 No. 7 (2015): Vol 27 Issue 7, 2015

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Optimized Solid-Phase Extraction of Astaxanthin from Portunus trituberculatus Using Response Surface Methodology and Ionic Liquid-Based Sorbents

https://doi.org/10.14233/ajchem.2015.18850
Y.R. Lee
Department of Chemistry and Chemical Engineering, Inha University, 253, Yonghyun-Dong, Nam Gu, Incheon 402751, Republic of Korea
Y.J. Lee
Department of Chemistry and Chemical Engineering, Inha University, 253, Yonghyun-Dong, Nam Gu, Incheon 402751, Republic of Korea
K.H. Row
Department of Chemistry and Chemical Engineering, Inha University, 253, Yonghyun-Dong, Nam Gu, Incheon 402751, Republic of Korea

Corresponding Author(s) : K.H. Row

rowkho@inha.ac.kr

Asian Journal of Chemistry, Vol. 27 No. 7 (2015): Vol 27 Issue 7, 2015

Abstract

A 17-run Box-Behnken design was used to optimize the extraction conditions of astaxanthin from Portunus trituberculatus. The effects of the extraction time (h), extraction temperature (°C) and liquid to solid ratio were investigated. The following optimal conditions were derived using a response surface methodology: extraction time = 2 h, extraction temperature = 83 °C and ratio of liquid to solid = 10:1. The theoretical amount of astaxanthin (29.34 μg g-1) was obtained under the above conditions using Design-Expert software. The solid-phase extraction method was used to purify astaxanthin from Portunus trituberculatus using ionic liquid-based silica and polymer, such as SilprMImCl, SilprBimCl, SilprNH2, PEImCl, PBImCl and PHImCl sorbents. The optimal solid-phase extraction conditions were water as the washing solvent and ethanol as the elution solvent. Under these conditions, 18.3 μg g-1 astaxanthin was obtained, which was in good agreement with the value predicted from the Box-Behnken design model.

Keywords

Purification Solid phase extraction Astaxanthin Response surface methodology
Lee, Y., Lee, Y., & Row, K. (2015). Optimized Solid-Phase Extraction of Astaxanthin from Portunus trituberculatus Using Response Surface Methodology and Ionic Liquid-Based Sorbents. Asian Journal of Chemistry, 27(7), 2691–2698. https://doi.org/10.14233/ajchem.2015.18850
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