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Vol. 31 No. 1 (2019): Vol 31 Issue 1

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Oxidative Cleavage of DNA by Transition Metal Complexes: Synthesis, Spectral Characterization and DNA Interactions of Copper(II) Complexes with Quinquedentate Schiff Base Ligands

https://doi.org/10.14233/ajchem.2019.21558
M. Pragathi
Department of Chemistry, Sri Vishnu Gavimatam Government Degree College, Kalyandurg-515761, India
K. Hussain Reddy
Department of Chemistry, Sri Krishnadevaraya University, Anantapuramu-515003, India

Corresponding Author(s) : M. Pragathi

pragathi.madur@gmail.com

Asian Journal of Chemistry, Vol. 31 No. 1 (2019): Vol 31 Issue 1

Abstract

Designing of dinucleating ligands, with an additional donor atom that can bridge two metals in a more or less fixed geometry has rapidly developed in recent years. Part of the interest stems from the fact that the corresponding complexes are often studied as enzyme mimics. Two quinquedentate ligands have been synthesized by condensing salicylaldehyde/o-hydroxy- acetophenone with 2-hydroxy-1,3- propanediamine. The ligands and their metal complexes are synthesized and characterized by physicochemical and spectral analysis. Electrochemical behaviour of the complexes is investigated through cyclic voltammetric studies. E1/2 values are observed at 0.360 and 0.331 V vs. Ag/AgCl for the complexes. The non-equivalent current in cathodic and anodic peaks (ic/ia = 1.224 and 1.065 at 100 mV s-1) for metal complexes indicate quasi-reversible behaviour. Binding interactions of the dinuclear copper(II) complexes with calf thymus DNA are investigated using absorption spectrophotometry. Cleavage activities of these complexes are uncovered on a double stranded pBR plasmid DNA by using gel electrophoresis experiments in different conditions. At micromolar concentration, the ligands exhibit no significant activity, whereas the metal complexes show significantly enhanced nuclease activity due to the presence of metal ions. Copper complexes cleave DNA more effectively in the presence of oxidant. This is consistent with the increased production of hydroxyl radicals by cuprous ions similar to the well known “Fenton reaction”.

Keywords

Oxidative cleavage of DNA Quinquedentate ligands Copper(II) complexes Spectrophotometry Fenton reaction
Pragathi, M., & Reddy, K. H. (2018). Oxidative Cleavage of DNA by Transition Metal Complexes: Synthesis, Spectral Characterization and DNA Interactions of Copper(II) Complexes with Quinquedentate Schiff Base Ligands. Asian Journal of Chemistry, 31(1), 148–156. https://doi.org/10.14233/ajchem.2019.21558
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