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Vol. 27 No. 4 (2015): Vol 27 Issue 4

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Simple, Sensitive and Rapid Ultra-High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry for the Determination of Nicotine and its Nine Metabolites in Human Urine

https://doi.org/10.14233/ajchem.2015.18048
Zhong Fan
Techonology Centre, China Tobacco Guangxi Industrial Co. Ltd., Nanning 530001, P.R. China
Huimin Liu
Chemistry Department, Zhengzhou Tobacco Research Institute of CNTC, Zhenzhou 450001, P.R. China
Fuwei Xie
Chemistry Department, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
Qiaoling Xia
Chemistry Department, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
Sheng Wang
Chemistry Department, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
Zhiyan Chen
Techonology Centre, China Tobacco Guangxi Industrial Co. Ltd., Nanning 530001, P.R. China
Xiaolan Li
Techonology Centre, China Tobacco Guangxi Industrial Co. Ltd., Nanning 530001, P.R. China

Corresponding Author(s) : Xiaolan Li

xiaolan-dg@163.com

Asian Journal of Chemistry, Vol. 27 No. 4 (2015): Vol 27 Issue 4

Abstract

A simple, sensitive and rapid UHPLC-MS/MS method was developed for the quantication of nicotine and its nine metabolites in human urine. The assay only involves centrifugation and filtration of four times diluted urine, analysis was performed on an Agilent RRHD Eclipse XDB-C18 column (50 mm × 2.1 mm i.d., 1.8 μm, Agilent) using a gradient of 10 mM ammonium acetate, pH 6.8 and methanol as a mobile phase at a flow rate of 200 μL min-1. Separated compounds were determined by atmospheric pressure chemical ionization (APCI) tandem mass spectrometry in the positive ion mode using MRM. The results showed that: recoveries for nicotine and nine of its major metabolites ranged from 93.2-103.8 %; RSD for all the compounds were between 0.9 and 9.5 %. The described method was suitable for determining the nicotine dose in large-scale human bio-monitoring studies.

Keywords

UHPLC-MS/MS Nicotine Metabolites Human urine
Fan, Z., Liu, H., Xie, F., Xia, Q., Wang, S., Chen, Z., & Li, X. (2015). Simple, Sensitive and Rapid Ultra-High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry for the Determination of Nicotine and its Nine Metabolites in Human Urine. Asian Journal of Chemistry, 27(4), 1403–1407. https://doi.org/10.14233/ajchem.2015.18048
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References
  1. D. Yildiz, Toxicon, 43, 619 (2004); doi:10.1016/j.toxicon.2004.01.017.
  2. A.R. Triker, Beitr. Tabakforsch Int., 22, 147 (2006).
  3. C. Boswell, M. Curvall, R.K. Elswick Jr and D. Leyden, Biomarkers, 5, 341 (2000).
  4. E. Bruckert, N. Jacob, L. Lamaire, J. Truffert, F. Percheron and J.L. de Gennes, Clin. Chem., 38, 1705 (1992).
  5. J.J. Langone, H.B. Gjika and H. Van Vunakis, Use of Immunoassay Techniques for the Determination of Nicotine and its Metabolites, in eds.: J.W. Gorrod and P. Jacob III, Analytical Determination of Nicotine and Related Compounds and Their Metabolites, Elsevier, Amsterdam, pp. 265-283 (1999).
  6. R. Heinrich-Ramm, R. Wegner, A.H. Garde and X. Baur, Int. J. Hyg. Environ. Health, 205, 493 (2002); doi:10.1078/1438-4639-00173.
  7. R.J. Bjercke, G. Cook, N. Rychlik, H.B. Gjika, H. Van Vunakis and J.J. Langone, J. Immunol. Methods, 90, 203 (1986); doi:10.1016/0022-1759(86)90077-3.
  8. P. Jacob III, M. Wilson and N.L. Benowitz, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 222, 61 (1981).
  9. M.S. Jaakkola, J. Ma, G. Yang, M.F. Chin, N.L. Benowitz, M. Ceraso and J.M. Samet, Prev. Med., 36, 282 (2003); doi:10.1016/S0091-7435(02)00037-3.
  10. H. Chen, L. Yang, W. Yu and X. Yan, Anal. Methods, 4, 863 (2012).
  11. D.C.M. Bordin, M.N.R. Alves, O.G. Cabrices, E.G. de Campos and B.S. De Martinis, J. Anal. Toxicol., 38, 31 (2014); doi:10.1093/jat/bkt092.
  12. C. Feyerabend and M.A.H. Russell, J. Pharm. Pharmacol., 42, 450 (1990); doi:10.1111/j.2042-7158.1990.tb06592.x.
  13. O.A. Ghosheh, D. Browne, T. Rogers, J. de Leon, L.P. Dwoskin and P.A. Crooks, J. Pharm. Biomed. Anal., 23, 543 (2000); doi:10.1016/S0731-7085(00)00339-3.
  14. M. Page-Sharp, T.W. Hale, L.P. Hackett, J.H. Kristensen and K.F. Ilett, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 796, 173 (2003); doi:10.1016/j.jchromb.2003.08.020.
  15. M.C. Bentley, M. Abrar, M. Kelk, J. Cook and K. Phillips, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 723, 185 (1999); doi:10.1016/S0378-4347(98)00494-0.
  16. T. Tuomi, T. Johnsson and K. Reijula, Clin. Chem., 45, 2164 (1999).
  17. M. Meger, I. Meger-Kossien, A. Schuler-Metz, D. Janket and G. Scherer, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 778, 251 (2002); doi:10.1016/S0378-4347(01)00451-0.
  18. X. Xu, M.M. Iba and C.P. Weisel, Clin. Chem., 50, 2323 (2004); doi:10.1373/clinchem.2004.038489.
  19. D.L. Heavner, J.D. Richardson, W.T. Morgan and M.W. Ogden, Biomed. Chromatogr., 19, 312 (2005); doi:10.1002/bmc.463.
  20. J. Kuhn, T. Vollmer, C. Martin, D. Hendig and C. Knabbe, J. Pharm. Biomed. Anal., 67, 137 (2012); doi:10.1016/j.jpba.2012.04.036.
  21. M. Piller, G. Gilch, G. Scherer and M. Scherer, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 951, 7 (2014); doi:10.1016/j.jchromb.2014.01.025.
  22. R. Dams, M.A. Huestis, W.E. Lambert and C.M. Murphy, J. Am. Soc. Mass Spectrom., 14, 1290 (2003); doi:10.1016/S1044-0305(03)00574-9.
  23. F. Adlkofer, G. Scherer, A. Biber, W.D. Heller, P.N. Lee and H. Schievelbein, in eds.: N. Wald and P. Froggart, Consistency of Nicotine Intake in Smokers of Cigarettes with Varying Nicotine Yields, In: Nicotine, Smoking and the Low Tar Programme, Oxford University Press, Oxford, p. 130 (1989).
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