Study on Interaction Between Cefixime and Bovine Serum Albumin by Modified Fluorescence Spectroscopy

Lihui Zhang; Baosheng Liu; Zhiyun Li; Ying Guo

doi:10.14233/ajchem.2015.18289

Issue

Vol. 27 No. 4 (2015): Vol 27 Issue 4

Issue Published : February 4, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Study on Interaction Between Cefixime and Bovine Serum Albumin by Modified Fluorescence Spectroscopy

https://doi.org/10.14233/ajchem.2015.18289

Lihui Zhang

Department of Chemistry, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P.R. China

Baosheng Liu

Department of Chemistry, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P.R. China

Zhiyun Li

Department of Chemistry, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P.R. China

Ying Guo

Department of Chemistry, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P.R. China

Corresponding Author(s) : Baosheng Liu

lbs@hbu.edu.cn

Asian Journal of Chemistry, Vol. 27 No. 4 (2015): Vol 27 Issue 4
Article Published : February 4, 2015

Abstract

The reaction mechanism between cefixime (CFX) and bovine serum albumin (BSA) at different temperature was investigated by the classical fluorescence spectroscopy with focus on the fluorescence change of protein, as well as the elastic scattering fluorescence spectroscopy with focus on the fluorescence change of drug. The results indicated that cefixime could quench the intrinsic fluorescence of bovine serum albumin strongly through a static quenching process. The electrostatic force played an important role on the conjugation reaction between cefixime and bovine serum albumin, the number of binding site (n) in the binary system was approximately equal to 1. The binding constant obtained from elastic scattering fluorescence spectroscopy was larger than the one obtained from classical fluorescence spectroscopy by two orders of magnitude for the CFX-BSA system. At last the correctness of elastic scattering fluorescence spectroscopy method was verified by UV-visible absorption spectroscopy. It is also speculated that "point to side" interaction between drugs and peptides was existed.

Keywords

Fluorescence spectroscopy Elastic scattering fluorescence Cefixime Bovine serum albumin Interaction

Zhang, L., Liu, B., Li, Z., & Guo, Y. (2015). Study on Interaction Between Cefixime and Bovine Serum Albumin by Modified Fluorescence Spectroscopy. Asian Journal of Chemistry, 27(4), 1449–1452. https://doi.org/10.14233/ajchem.2015.18289

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References

A. Varlan and M. Hillebrand, Rev. Roum. Chim., 55, 69 (2010).

X.R. Pan, R.T. Liu, P.F. Qin, L. Wang and X.C. Zhao, J. Lumin., 130, 611 (2010); doi:10.1016/j.jlumin.2009.11.004.

C.F. Bohren and D.R. Hufillann, Absorption and Scattering Light by Smal1 Particles, John Wiley & Sons, New York (1983).

T. Tan, R. Huang and Z.N. Xia, Chin. J. Anal. Chem., 35, 1415 (2007).

S.F. Yu, World Notes on Antibiotics, 16, 339 (1995).

I. Matei and M. Hillebrand, J. Pharm. Biomed. Anal., 51, 768 (2010); doi:10.1016/j.jpba.2009.09.037.

Y.J. Hu, Y.O. Yang, A.M. Bai, W. Li and Y. Liu, J. Solution Chem., 39, 709 (2010); doi:10.1007/s10953-010-9527-8.

N. Barbero, E. Barni, C. Barolo, P. Quagliotto, G. Viscardi, L. Napione, S. Pavan and F. Bussolino, Dyes Pigments, 80, 307 (2009); doi:10.1016/j.dyepig.2008.08.006.

Y.L. Wei, J.Q. Li, C. Dong, S.M. Shuang, D.S. Liu and C.W. Huie, Talanta, 70, 377 (2006); doi:10.1016/j.talanta.2006.02.052.

S.A. Markarian and M.G. Aznauryan, Mol. Biol. Rep., 39, 7559 (2012).

C.Z. Zheng, H.P. Wang, W. Xu, C.Y. Xu, J.G. Liang and H.Y. Han, Spectrochim. Acta A, 118, 897 (2014); doi:10.1016/j.saa.2013.09.082.

F. Wang, W. Huang, B. Tang and X.J. Liu, Chin. J. Anal. Chem., 34, S239 (2006).

R. Huang, Z.N. Xia and P. Gong, Spectrosc. Spect. Anal, 28, 161 (2008).

Y.M. Song, Z. Liu, K.J. Wang and N.N. Luan, Acta Chim. Sin., 68, 2191 (2010).

P. Ju, H. Fan, T. Liu, L. Cui, S.Y. Ai and X.N. Wu, Biol. Trace Elem. Res., 144, 1405 (2011); doi:10.1007/s12011-011-9090-1.

J. Mariam, P.M. Dongre and D.C. Kothari, J. Fluoresc., 21, 2193 (2011); doi:10.1007/s10895-011-0922-3.

P. Sen, S. Fatima, B. Ahmad and R.H. Khan, Spectrochim. Acta A, 74, 94 (2009); doi:10.1016/j.saa.2009.05.010.

Issue

Vol. 27 No. 4 (2015): Vol 27 Issue 4

Study on Interaction Between Cefixime and Bovine Serum Albumin by Modified Fluorescence Spectroscopy

Corresponding Author(s) : Baosheng Liu

Abstract

Keywords

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