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Vol. 27 No. 4 (2015): Vol 27 Issue 4

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Simultaneous Determination of Baicalin and Berberine Hydrochloride in Fufangkangkui Suspension by HPLC

https://doi.org/10.14233/ajchem.2015.18087
De-Zhou Ge
Bengbu Institute for Food and Drug Control, Bengbu 233030, Anhui Province, P.R. China
Hao Zhong
Bengbu Institute for Food and Drug Control, Bengbu 233030, Anhui Province, P.R. China
Zu-Lei Deng
Faculty of Pharmacy, Bengbu Medical College, Bengbu 233000, Anhui Province, P.R. China
Liu Hao
Bengbu Institute for Food and Drug Control, Bengbu 233030, Anhui Province, P.R. China

Corresponding Author(s) : Liu Hao

734387011@qq.com

Asian Journal of Chemistry, Vol. 27 No. 4 (2015): Vol 27 Issue 4

Abstract

A high-performance liquid chromatography method with diode array detection was developed for simultaneous analysis of baicalin and berberine hydrochloride in Fufangkangkui suspension, a traditional Chinese medicine being prescribed widely for antibacterial, antiviral and immunostimulant activity. A relatively simple extraction procedure was employed and optimized and separation of the components was obtained within 0.5 h using an Agilent ZORBAX Eclipse XDB-C18 column under gradient elution with acetonitrile and a buffer containing 6 mmol/L KH2PO4 solutions (pH = 4.6). All calibration curves showed good linear correlation (r2 > 0.9997) within the test ranges. Validation proved that the repeatability of the method was good and recovery was satisfactory. The HPLC method was able rapidly and efficiently to analyze baicalin and berberine hydrochloride in Fufangkangkui suspension. In conclusion, these results demonstrated that the method proposed was useful for the analysis and quality consistency evaluation of Fufangkangkui suspension.

Keywords

Baicalin Berberine hydrochloride Fufangkangkui suspension HPLC
Ge, D.-Z., Zhong, H., Deng, Z.-L., & Hao, L. (2015). Simultaneous Determination of Baicalin and Berberine Hydrochloride in Fufangkangkui Suspension by HPLC. Asian Journal of Chemistry, 27(4), 1427–1430. https://doi.org/10.14233/ajchem.2015.18087
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