Comparative Studies on the Interaction Between the Acriflavine and Bovine Serum Albumin by Fluorescence Spectroscopy, Synchronous Fluorescence

Ying Guo; Baosheng Liu; Zhiyun Li; Lihui Zhang

doi:10.14233/ajchem.2015.18313

Issue

Vol. 27 No. 4 (2015): Vol 27 Issue 4

Issue Published : February 4, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Comparative Studies on the Interaction Between the Acriflavine and Bovine Serum Albumin by Fluorescence Spectroscopy, Synchronous Fluorescence

https://doi.org/10.14233/ajchem.2015.18313

Ying Guo

College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P.R. China

Baosheng Liu

College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P.R. China

Zhiyun Li

College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P.R. China

Lihui Zhang

College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P.R. China

Corresponding Author(s) : Baosheng Liu

lbs@hbu.edu.cn

Asian Journal of Chemistry, Vol. 27 No. 4 (2015): Vol 27 Issue 4
Article Published : February 4, 2015

Abstract

Under simulated physiological conditions, we studied the reaction mechanism of acriflavine (ACF) with bovine serum albumin (BSA) at different temperature by utilizing fluorescence quenching method and synchronous fluorescence method, respectively. The results indicate that acriflavine could quench the intrinsic fluorescence of bovine serum albumin strongly and the quenching mechanism was a static quenching process; the electrostatic force played an important role on the conjugation reaction between acriflavine and bovine serum albumin. The results obtained by the two methods were consistent, which indicated synchronous fluorescence spectroscopy can replace traditional fluorescence quenching method to study reaction mechanism of dyes with proteins.

Keywords

Fluorescence spectroscopy Synchronous fluorescence Acriflavine Bovine serum albumin Interaction

Guo, Y., Liu, B., Li, Z., & Zhang, L. (2015). Comparative Studies on the Interaction Between the Acriflavine and Bovine Serum Albumin by Fluorescence Spectroscopy, Synchronous Fluorescence. Asian Journal of Chemistry, 27(4), 1453–1456. https://doi.org/10.14233/ajchem.2015.18313

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References

J. Guharay, B. Sengupta and P.K. Sengupta, Proteins, 43, 75 (2001); doi:10.1002/1097-0134(20010501)43:2<75::AID-PROT1019>3.0.CO;2-7.

C. Bertucci and E. Domenici, Curr. Med. Chem., 9, 1463 (2002); doi:10.2174/0929867023369673.

A. Tamta, M. Chaudhary and R. Sehgal, Int. J. Pharm., 6, 111 (2010); doi:10.3923/ijp.2010.111.116.

H.H. Eldaroti, S.A. Gadir, M.S. Refat and A.M.A. Adam, Int. J. Electrochem. Soc., 8, 5774 (2013).

B. Ahmad, S. Parveen and R.H. Khan, Biomacromolecules, 7, 1350 (2006); doi:10.1021/bm050996b.

S. Deepa and A.K. Mishra, J. Pharm. Biomed. Anal., 38, 556 (2005); doi:10.1016/j.jpba.2005.01.023.

E.L. Gelamo, C.H. Silva, H. Imasato and M. Tabak, Biochim. Biophys. Acta, 1594, 84 (2002); doi:10.1016/S0167-4838(01)00287-4.

J. Zhang, W.X. Li, B.Y. Ao, S.Y. Feng and X.D. Xin, Spectrochim. Acta A, 118, 972 (2014); doi:10.1016/j.saa.2013.09.135.

Y.N. Zhu, B. Li, H.A. Yin, S.G. Ge and J.H. Yu, Monatsh. Chem., 145, 167 (2014); doi:10.1007/s00706-013-0991-x.

J.C. Li, N. Li, Q.H. Wu, Z. Wang, J.J. Ma, C. Wang and L.J. Zhang, J. Mol. Struct., 833, 184 (2007); doi:10.1016/j.molstruc.2006.09.019.

Y.Z. Zhang, X.X. Chen, J. Dai, X.P. Zhang, Y.X. Liu and Y. Liu, Luminescence, 23, 150 (2008); doi:10.1002/bio.1025.

O. Azimi, Z. Emami, H. Salari and J. Chamani, Molecules, 16, 9792 (2011); doi:10.3390/molecules16129792.

Q. Zhang, Y. Ni and S. Kokot, J. Pharm. Biomed. Anal., 52, 280 (2010); doi:10.1016/j.jpba.2010.01.006.

P.D. Ross and S. Subramanian, Biochemistry, 20, 3096 (1981); doi:10.1021/bi00514a017.

Y. Teng, R.T. Liu, C. Li, Q. Xia and P.J. Zhang, J. Hazard. Mater., 190, 574 (2011); doi:10.1016/j.jhazmat.2011.03.084.

C.Z. Zheng, H.P. Wang, W. Xu, C.Y. Xu, J.G. Liang and H.Y. Han, Spectrochim. Acta A, 118, 897 (2014); doi:10.1016/j.saa.2013.09.082.

Issue

Vol. 27 No. 4 (2015): Vol 27 Issue 4

Comparative Studies on the Interaction Between the Acriflavine and Bovine Serum Albumin by Fluorescence Spectroscopy, Synchronous Fluorescence

Corresponding Author(s) : Baosheng Liu

Abstract

Keywords

Full Article

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