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Variations of Macro-compounds in Cyanobacterium, Thermosynchococcus elongatus CL-1 Under Various Nutrients
Corresponding Author(s) : Hsinta Hsueh
Asian Journal of Chemistry,
Vol. 27 No. 3 (2015): Vol 27 Issue 3
Abstract
The photosynthetic algae, Thermosynchococcus elongatus CL-1 (TCL-1) purified from an alkaline hot spring (pH 9.3, 62 °C) in eastern Taiwan was applied to enhance the overall CO2 absorption capacity in aqueous solution. It was observed that the Thermosynchococcus elongatus CL-1 can be experimentally cultivated over pH 12 and suggested its high affinity of bicarbonate and adapted ability of alkaline condition. A series of the variations of macro-compounds, including lipids, carbohydrates, proteins, pigments and element compositions (C, H and N) were determined under a batch cultivation at pH 9. Additionally, a combined method with Fourier transform infrared spectrum and curve fitting as well as a wet chemistry method was used to determine the variations of lipids.
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- D.A. Stainforth, T. Aina, C. Christensen, M. Collins, N. Faull, D.J. Frame, J.A. Kettleborough, S. Knight, A. Martin, J.M. Murphy, C. Piani, D. Sexton, L.A. Smith, R.A. Spicer, A.J. Thorpe and M.R. Allen, Nature, 433, 403 (2005); doi:10.1038/nature03301.
- H.T. Hsueh, H. Chu and C.C. Chang, Environ. Sci. Technol., 41, 1909 (2007); doi:10.1021/es0620639.
- K. Stehfest, J. Toepel and C. Wilhelm, Plant Physiol. Biochem., 43, 717 (2005); doi:10.1016/j.plaphy.2005.07.001.
- H.J.R. Stevenson and S. Levine, Science, 116, 705 (1952); doi:10.1126/science.116.3026.705.
- D.Y. Tseng, R. Vir, S.J. Traina and J.J. Chalmers, Biotechnol. Bioeng., 52, 661 (1996); doi:10.1002/(SICI)1097-0290(19961220)52:6<661::AID-BIT4>3.3.CO;2-5.
- A.L Smith, Spectroscopy, Fundamentals, Techniques and Analytical Problem-Solving; Wiley: New York (1979).
- H.T. Hsueh, H. Chu and S.C. Yu, Chemosphere, 66, 878 (2007); doi:10.1016/j.chemosphere.2006.06.022.
- T. Takeuchi, K. Utsunomiya, K. Kobayashi, M. Owada and I. Karube, J. Biotechnol., 25, 261 (1992); doi:10.1016/0168-1656(92)90160-B.
- O. Holm-Hansen, J. Coombs, B.E. Volcani and P.M. Williams, Anal. Biochem., 19, 561 (1967); doi:10.1016/0003-2697(67)90247-3.
- M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith, Anal. Chem., 28, 350 (1956); doi:10.1021/ac60111a017.
- G.D. Price, F.J. Woodger, M.R. Badger, S.M. Howitt and L. Tucker, Proc. Natl. Acad. Sci. USA, 101, 18228 (2004); doi:10.1073/pnas.0405211101.
References
D.A. Stainforth, T. Aina, C. Christensen, M. Collins, N. Faull, D.J. Frame, J.A. Kettleborough, S. Knight, A. Martin, J.M. Murphy, C. Piani, D. Sexton, L.A. Smith, R.A. Spicer, A.J. Thorpe and M.R. Allen, Nature, 433, 403 (2005); doi:10.1038/nature03301.
H.T. Hsueh, H. Chu and C.C. Chang, Environ. Sci. Technol., 41, 1909 (2007); doi:10.1021/es0620639.
K. Stehfest, J. Toepel and C. Wilhelm, Plant Physiol. Biochem., 43, 717 (2005); doi:10.1016/j.plaphy.2005.07.001.
H.J.R. Stevenson and S. Levine, Science, 116, 705 (1952); doi:10.1126/science.116.3026.705.
D.Y. Tseng, R. Vir, S.J. Traina and J.J. Chalmers, Biotechnol. Bioeng., 52, 661 (1996); doi:10.1002/(SICI)1097-0290(19961220)52:6<661::AID-BIT4>3.3.CO;2-5.
A.L Smith, Spectroscopy, Fundamentals, Techniques and Analytical Problem-Solving; Wiley: New York (1979).
H.T. Hsueh, H. Chu and S.C. Yu, Chemosphere, 66, 878 (2007); doi:10.1016/j.chemosphere.2006.06.022.
T. Takeuchi, K. Utsunomiya, K. Kobayashi, M. Owada and I. Karube, J. Biotechnol., 25, 261 (1992); doi:10.1016/0168-1656(92)90160-B.
O. Holm-Hansen, J. Coombs, B.E. Volcani and P.M. Williams, Anal. Biochem., 19, 561 (1967); doi:10.1016/0003-2697(67)90247-3.
M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith, Anal. Chem., 28, 350 (1956); doi:10.1021/ac60111a017.
G.D. Price, F.J. Woodger, M.R. Badger, S.M. Howitt and L. Tucker, Proc. Natl. Acad. Sci. USA, 101, 18228 (2004); doi:10.1073/pnas.0405211101.