Analysis of 5,8,11,14,17-Eicosapentaenoic Acid and 4,7,10,13,16,19-Docosahexaenoic Acid in the Viscera of Marine Organisms Using Gas Chromatography

Deyong Zhang; Xiaolu Xu; Xiu-Ying Shen; Huiying Xu

doi:10.14233/ajchem.2015.17124

Issue

Vol. 27 No. 3 (2015): Vol 27 Issue 3

Issue Published : January 19, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Analysis of 5,8,11,14,17-Eicosapentaenoic Acid and 4,7,10,13,16,19-Docosahexaenoic Acid in the Viscera of Marine Organisms Using Gas Chromatography

https://doi.org/10.14233/ajchem.2015.17124

Deyong Zhang

College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, P.R. China

Xiaolu Xu

College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, P.R. China

Xiu-Ying Shen

College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, P.R. China

Huiying Xu

College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, P.R. China

Corresponding Author(s) : Deyong Zhang

deyonghz@163.com

Asian Journal of Chemistry, Vol. 27 No. 3 (2015): Vol 27 Issue 3
Article Published : January 18, 2015

Abstract

A gas chromatography was established for analysis of 5,8,11,14,17-eicosapentaenoic acid and 4,7,10,13,16,19-docosahexaenoic acid in the viscera of marine organisms. The viscera of 6 kinds of non-fish marine organisms were then collected for oil extraction and detection of 4,7,10,13,16,19-docosahexaenoic acid and 5,8,11,14,17-eicosapentaenoic acid. The oil extraction ratio varied from 0.72 to 6.23 % (wt. %). Scylla serrata presented the highest oil yield, followed by Architeuthis dux and Panopea abrupta. The 5,8,11,14,17-eicosapentaenoic acid concentration (in methyl ester form) in the oil varied from 0.56 to 7.75 (mg/g). Scylla serrata presented the highest 5,8,11,14,17-eicosapentaenoic acid concentration (p < 0.05), followed by Architeuthis dux and Panopea abrupta. The 4,7,10,13,16,19-docosahexaenoic acid concentration(in methyl ester form) varied from 0.15 to 4.71 (mg/g). Architeuthis dux and Scylla serrata presented obviously higher 4,7,10,13,16,19-docosahexaenoic acid concentration than other species (p < 0.05).

Keywords

Marine organisms Viscera EPA DHA Gas chromatography

Zhang, D., Xu, X., Shen, X.-Y., & Xu, H. (2015). Analysis of 5,8,11,14,17-Eicosapentaenoic Acid and 4,7,10,13,16,19-Docosahexaenoic Acid in the Viscera of Marine Organisms Using Gas Chromatography. Asian Journal of Chemistry, 27(3), 833–836. https://doi.org/10.14233/ajchem.2015.17124

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References

F. Russell and C. Bürgin-Maunder, Mar. Drugs, 10, 2535 (2012); doi:10.3390/md10112535.

W.S. Harris, T.D. Dayspring and T.J. Moran, Postgrad. Med., 125, 100 (2013); doi:10.3810/pgm.2013.11.2717.

A.M. Minihane, Eur. J. Clin. Nutr., 67, 536 (2013); doi:10.1038/ejcn.2013.19.

T. Nicholson, H. Khademi and M.H. Moghadasian, Food Funct., 4, 357 (2013); doi:10.1039/c2fo30235g.

C.I.F. Janssen and A.J. Kiliaan, Progr. Lipid Res., 53, 1 (2014); doi:10.1016/j.plipres.2013.10.002.

D.W. Luchtman and C. Song, Neuropharmacology, 64, 550 (2013); doi:10.1016/j.neuropharm.2012.07.019.

T. Kawakita, F. Kawabata, T. Tsuji, M. Kawashima, S. Shimmura and K. Tsubota, Biomed. Res., 34, 215 (2013); doi:10.2220/biomedres.34.215.

H.L. Ramkumar, J. Tuo, D.F. Shen, J. Zhang, X. Cao, E.Y. Chew and C.-C. Chan, Nutr., 143, 1129 (2013); doi:10.3945/jn.112.169649.

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