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Vol. 27 No. 3 (2015): Vol 27 Issue 3

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Interaction Characteristic Studies of Ciprofloxacin and/or Sulphadiazine with Bovine Serum Albumin by Spectroscopic Technique

https://doi.org/10.14233/ajchem.2015.17121
Xu-Yang Liu
College of Chemical and Environmental Science, Hebei University; Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China
Qian Wang
College of Chemical and Environmental Science, Hebei University; Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China
Zhi-Hong Shi
College of Chemical and Environmental Science, Hebei University; Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China
Xiang-Hua Xia
College of Chemical and Environmental Science, Hebei University; Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China
Han-Wen Sun
College of Chemical and Environmental Science, Hebei University; Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China

Corresponding Author(s) : Han-Wen Sun

sunhanwenhbdx@126.com

Asian Journal of Chemistry, Vol. 27 No. 3 (2015): Vol 27 Issue 3

Abstract

In this paper, the interaction of ciprofloxacin (CPFX) and/or sulphadiazine (SD) with bovine serum albumin (BSA) under simulative physiological conditions was studied, by fluorescence quenching in combination with UV-visible spectroscopic method. The fluorescence quenching constants, binding distance and binding constants for BSA-CPFX and/or sulphadiazine systems were determined. The fluorescence quenching of BSA by addition of ciprofloxacin and/or sulphadiazine is due to static quenching and energy transfer. The binding constants (KA) of BSA-SD and BSA-CPFX systems were 4.04 × 106 and 6.96 × 105 M-1, respectively, showing that sulphadiazine has higher binding capability with bovine serum albumin than ciprofloxacin. In the presence of sulphadiazine or ciprofloxacin, the KA values of BSA-CPFX or BSA-SD systems decreased to 4.63 × 103 or 6.56 × 104 M-1, suggesting that free ciprofloxacin and sulphadiazine in blood increased in their co-presence. Circular dichroism spectra, synchronous fluorescence and three-dimensional fluorescence studies showed that the presence of ciprofloxacin and/or sulphadiazine could change the conformation of bovine serum albumin during the binding process. The results are of great importance in pharmacy, pharmacology and biochemistry.

Keywords

Fluorescence Bovine serum albumin Ciprofloxacin Sulphadiazine Interaction characteristics
Liu, X.-Y., Wang, Q., Shi, Z.-H., Xia, X.-H., & Sun, H.-W. (2015). Interaction Characteristic Studies of Ciprofloxacin and/or Sulphadiazine with Bovine Serum Albumin by Spectroscopic Technique. Asian Journal of Chemistry, 27(3), 818–826. https://doi.org/10.14233/ajchem.2015.17121
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