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Vol. 27 No. 3 (2015): Vol 27 Issue 3

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Determination of 5-Fluorouracil in its Injection and Biological Fluid by Enhanced Chemiluminescence Based on Luminol-Ag(III) Complex Reaction in Alkaline Solution

https://doi.org/10.14233/ajchem.2015.17120
Ya-Juan Dong
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China
Ting Wang
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China
Pei-Yun Chen
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China
Han-Wen Sun
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China

Corresponding Author(s) : Han-Wen Sun

sunhanwenhbdx@126.com

Asian Journal of Chemistry, Vol. 27 No. 3 (2015): Vol 27 Issue 3

Abstract

A novel chemiluminescence method was developed for the determination of 5-fluorouracil based on the chemiluminescence reaction between Ag(III) complex [Ag(HIO6)2]5- and luminol in alkaline solution. Chemiluminescence emission of [Ag(HIO6)2]5--luminol in alkaline medium was different from that in acidic medium. A possible mechanism of enhanced chemiluminescence emission was suggested. The enhanced effect of 5-fluorouracil on chemiluminescence emission of the [Ag(HIO6)2]5--luminol system was found. The effect of the reaction conditions on chemiluminescence emission was examined and optimized. The chemiluminescence intensity was proportional to the logarithm of 5-fluorouracil concentration with correlation coefficient (r) of 0.9954 in the range of 10-5000 ng/mL. Under the optimized conditions, the limit of detection (LOD) was 3 ng/mL. The chemiluminescence system was applied for the determination of 5-fluorouracil in its injection and biological fluid with the recoveries of 94.6-104 % and with the RSD of 1.2-2.8 %. The proposed flow-injection chemiluminescence method provides high sensitivity necessary for analysis of 5-fluorouracil in pharmaceutical and biological fluids at clinically relevant concentrations.

Keywords

Enhanced chemiluminescence Flow injection 5-Fluorouracil Pharmaceutical analysis
Dong, Y.-J., Wang, T., Chen, P.-Y., & Sun, H.-W. (2015). Determination of 5-Fluorouracil in its Injection and Biological Fluid by Enhanced Chemiluminescence Based on Luminol-Ag(III) Complex Reaction in Alkaline Solution. Asian Journal of Chemistry, 27(3), 813–817. https://doi.org/10.14233/ajchem.2015.17120
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