Separation of Lipopolvsaccharides and Heparin Sodium by Activated Charcoal Adsorption and Ultrafiltration Process

C.Y. Li; Y.F. Nai; X. Qian; H.Y. Li; G.P. Peng

doi:10.14233/ajchem.2015.17857

Issue

Vol. 27 No. 3 (2015): Vol 27 Issue 3

Issue Published : January 19, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Separation of Lipopolvsaccharides and Heparin Sodium by Activated Charcoal Adsorption and Ultrafiltration Process

https://doi.org/10.14233/ajchem.2015.17857

C.Y. Li

Pharmacy College, Nanjing University of Chinese Medicine, Nanjing, P.R. China

Y.F. Nai

Pharmacy College, Nanjing University of Chinese Medicine, Nanjing, P.R. China

X. Qian

Pharmacy College, Nanjing University of Chinese Medicine, Nanjing, P.R. China

H.Y. Li

Pharmacy College, Nanjing University of Chinese Medicine, Nanjing, P.R. China

G.P. Peng

Pharmacy College, Nanjing University of Chinese Medicine, Nanjing, P.R. China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, P.R. China

Corresponding Author(s) : G.P. Peng

guopingpeng@126.com

Asian Journal of Chemistry, Vol. 27 No. 3 (2015): Vol 27 Issue 3
Article Published : January 18, 2015

Abstract

In this study, a harmful lipopolvsaccharides (endotoxin) was separated by membrane and activated charcoal from another polysaccharide with 12 kDa molecular weight, heparin sodium, which was widely used as an injectable anticoagulant. The concentration of endotoxin was investigated by dynamic turbidity method. Meanwhile, removal efficiency of endotoxin from heparin was 80.9, 5.8 and 98.8 % with 1.0 % (w/w) activated charcoal, 200 kDa polyether sulfone membrane and 200 kDa hybrid membrane, respectively. The loss of heparin was 15.7, 4.8 and 4.1 % correspondingly. Due to the similar polysaccharide of heparin and endotoxin, activated charcoal absorption may be limited by saturating and competitive adsorption. Compared with the endotoxin rejection and heparin retention, hybrid ultrafiltration membrane showed many advantages and also provided theoretical support for industrial manufacturing of heparin injection for removing endotoxin.

Keywords

Endotoxin Ultrafiltration Activated charcoal Hybrid Polythersulfone

Li, C., Nai, Y., Qian, X., Li, H., & Peng, G. (2015). Separation of Lipopolvsaccharides and Heparin Sodium by Activated Charcoal Adsorption and Ultrafiltration Process. Asian Journal of Chemistry, 27(3), 1005–1008. https://doi.org/10.14233/ajchem.2015.17857

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References

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References

M. Walensi, H. Groot, R. Schulz, M. Hartmann and F. Petrat, J. Surg. Res., 179, e57 (2013); doi:10.1016/j.jss.2012.01.002.

T.P. Lombardi, B. Gundersen, L.O. Zammett, J.K. Walters and B.A. Morris, Clin. Pharm., 7, 832 (1988).

J.E.A. Braun and D.L. Severson, Lipids, 28, 59 (1993); doi:10.1007/BF02536362.

J.P. Filkins and N.R.D. Luzio, Am. J. Phys., 214, 1074 (1968).

H. Fang, J. Wei and Y.T. Yu, Biomaterials, 25, 5433 (2004); doi:10.1016/j.biomaterials.2003.12.035.

D. Petsch and F.B. Anspach, J. Biotechnol., 76, 97 (2000); doi:10.1016/S0168-1656(99)00185-6.

M. Nagaki, R.D. Hughes, J.Y. Lau and R. Williams, Int. J. Artif. Organs, 14, 43 (1991).

J.L. Ding and B. Ho, Trends Biotechnol., 19, 277 (2001); doi:10.1016/S0167-7799(01)01694-8.

P.O. Magalhães, A.M. Lopes, P.G. Mazzola, C. Rangel-Yagui, T.C.V. Penna and A. Jr Pessoa, J. Pharm. Pharm. Sci., 10, 388 (2007).

E. Hannecart-Pokorni, D. Dekegel and F. Depuydt, Eur. J. Biochem., 38, 6 (1973); doi:10.1111/j.1432-1033.1973.tb03025.x.

B.S. Park, D.H. Song, H.M. Kim, B.S. Choi, H. Lee and J.O. Lee, Nature, 458, 1191 (2009); doi:10.1038/nature07830.

Gorbet and M.V. Sefton, Biomaterials, 26, 6811 (2005); doi:10.1016/j.biomaterials.2005.04.063.

J.H. Li, Y.G. Shao, Z.A. Chen, R.Z. Cong, J.D. Wang and X.L. Liu, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 791, 55 (2003); doi:10.1016/S1570-0232(03)00203-4.

M. Al Manasrah, M. Kallioinen, H. Ilvesniemi and M. Mänttäri, Bioresour. Technol., 114, 375 (2012); doi:10.1016/j.biortech.2012.02.014.

R. Schindler and C.A. Dinarello, J. Immunol. Methods, 116, 159 (1989); doi:10.1016/0022-1759(89)90199-3.

H.P. Wessel, M. Hosang, T.B. Tschopp and B.-J. Weimann, Carbohydr. Res., 204, 131 (1990); doi:10.1016/0008-6215(90)84028-S.

K.G. Ong, J.M. Leland, K.F. Zeng, G. Barrett, M. Zourob and C.A. Grimes, Biosens. Bioelectron., 21, 2270 (2006); doi:10.1016/j.bios.2005.11.007.

C. McLeod and W. Katz, J. Biol. Stand., 9, 299 (1981); doi:10.1016/S0092-1157(81)80055-8.

Issue

Vol. 27 No. 3 (2015): Vol 27 Issue 3

Separation of Lipopolvsaccharides and Heparin Sodium by Activated Charcoal Adsorption and Ultrafiltration Process

Corresponding Author(s) : G.P. Peng

Abstract

Keywords

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