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Vol. 27 No. 3 (2015): Vol 27 Issue 3

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Isolation and Identification of Flavonoids with Aldose Reductase Inhibitory Activity from Petasites japonicus

https://doi.org/10.14233/ajchem.2015.17845
Dong Gu Lee
Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea
Ki Ho Lee
Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea
Kwang-Woo Park
Department of Forest Resource Conservation, Korea National Arboretum, Pocheon 487-821, Republic of Korea
Chan Kyu Han
Korea Food Research Institute, Sungnam 463-746, Republic of Korea
Buom-Yong Ryu
Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea
Eun Ju Cho
Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Republic of Korea
Sanghyun Lee
Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea

Corresponding Author(s) : Sanghyun Lee

slee@cau.ac.kr

Asian Journal of Chemistry, Vol. 27 No. 3 (2015): Vol 27 Issue 3

Abstract

The purpose of this study was to evaluate the therapeutic potential of naturally occurring aldose reductase inhibitors extracted from Petasites japonicus. Methanol extract and stepwise polarity fractions of P. japonicus leaves were tested for aldose reductase inhibition on rat lenes in vitro. Of these, the ethyl acetate (EtOAc) fraction exhibited aldose reductase inhibitory activity (IC50 value, 0.26 μM). Chromatography of the active EtOAc fraction led to the further isolation of four flavonoids, identified as kaempferol-3-O-(6"-acetyl)-b-D-glucoside (1), quercetin-3-O-(6"-acetyl)-b-D-glucoside (2), kaempferol-3-O-b-D-glucoside (3) and quercetin-3-O-b-D-glucoside (4). Compounds 1-4 exhibited high aldose reductase inhibitory activity, with IC50 values of 6.08, 3.46, 8.55 and 2.21 μM, respectively. Compounds 1 and 2 were isolated for the first time from this plant and compound 4 showed the highest aldose reductase inhibitory activity. These results suggest that compound 4 extracted from P. japonicus is a potent aldose reductase inhibitor and could be a useful lead compound in the development of a novel aldose reductase inhibitory agent against diabetic complications.

Keywords

Aldose reductase inhibition Diabetic complication Flavonoid Petasites japonicus
Gu Lee, D., Ho Lee, K., Park, K.-W., Kyu Han, C., Ryu, B.-Y., Ju Cho, E., & Lee, S. (2015). Isolation and Identification of Flavonoids with Aldose Reductase Inhibitory Activity from Petasites japonicus. Asian Journal of Chemistry, 27(3), 991–994. https://doi.org/10.14233/ajchem.2015.17845
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