Issue

Vol. 26 No. 1 (2014): Vol 26 Issue 1

Copyright (c) 2014 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Spectrophotometric and Spectrofluorometric Determination of Doxazosin Mesylate in Tablets via Eosin Y

https://doi.org/10.14233/ajchem.2014.15315
Reda A. Ammar
Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia, Department of Chemistry, College of Science, Al-Azhar University, Cairo, Egypt
Amina M. El-Brashy
Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia
Tafla N. Al-Sahly
Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia

Corresponding Author(s) : Amina M. El-Brashy

aalbrashy@ksu.edu.sa

Asian Journal of Chemistry, Vol. 26 No. 1 (2014): Vol 26 Issue 1

Abstract

Doxazosin mesylate, an inhibitor of a-adrenergic receptors, was determined through formation of ion-pair complex with Eosin Y reagent in acetate buffer at pH 3. Two simple and sensitive spectroscopic methods were suggested. The first method based on spectrophotometric measurement of the complex at 547 nm and the second one based on spectrofluorimetric quenching measurement of the complex at 570 nm, after excitation at 340 nm. Under the optimum conditions, doxazosin mesylate was determined in the range of 2 - 14 μg mL-1 and 1-10 μg mL-1 by the spectrophotometric and spectrofluorimetric methods respectively, with good accuracy and precision in pure form. The method was successfully applied for determination of doxazosin mesylate in its tablets. All the results were statistically compared with that obtained by a reference method and subjected to validation analysis according to International Conference on Harmonization.

Keywords

Doxazosin mesylate Eosin Y Fluorometry Quenching Spectrophotometry Dosage forms
A. Ammar, R., M. El-Brashy, A., & N. Al-Sahly, T. (2013). Spectrophotometric and Spectrofluorometric Determination of Doxazosin Mesylate in Tablets via Eosin Y. Asian Journal of Chemistry, 26(1), 57–62. https://doi.org/10.14233/ajchem.2014.15315
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Health Mash, www.druglib.com and www.mayoclinic.com.
  2. Y.J. Kim, Y. Lee, M.J. Kang, J.S. Huh, M. Yoon, J. Lee and Y.W. Choi, Biomed. Chromatogr., 20, 1172 (2006); doi:10.1002/bmc.666.
  3. N. Ma, W. Liu, H. Li, B. Chen, Y. Zhu, X. Liu, F. Wang, D. Xiang and B. Zhang, J. Pharm. Biomed. Anal., 43, 1049 (2007); doi:10.1016/j.jpba.2006.09.013.
  4. M. Erceg, M. Cindric, L.P. Frketic, M. Vertzoni, B. Cetina-Cizmek and C. Reppas, J. Chromatogr. Sci., 48, 114 (2010); doi:10.1093/chromsci/48.2.114.
  5. B. Dhanya, A. Suganthi, A.K. Sen, U. Sahoo and A.K. Seth, J. Pharm. Sci., 73, 120 (2011).
  6. R.T. Sane, M. Francis, P.S. Hijli, S. Pawar and A.R. Pathak, J. Planar Chromatogr., 15, 34 (2002); doi:10.1556/JPC.15.2002.1.6.
  7. G. Altıokka and Z. Atkosa, J. Pharm. Biomed. Anal., 27, 841 (2002); doi:10.1016/S0731-7085(01)00516-7.
  8. G. Altiokka and M. Tunçel, J. Pharm. Biomed. Anal., 17, 169 (1998); doi:10.1016/S0731-7085(97)00211-2.
  9. H.P. Latha and G.V. Sagar, Der. Pharm. Res., 4, 1642 (2011).
  10. L.I. Bebawy, A.A. Moustafa and N.F. Abo-Talib, Pharm. Biomed. Anal., 27, 779 (2002); doi:10.1016/S0731-7085(01)00523-4.
  11. Z. Aydogmus and A. Barla, J. AOAC Int., 92, 131 (2009).
  12. A.M. El-Brashy, M.E.S. Metwally and F.A. El-Sepai, J. AOAC Int., 91, 1309 (2008).
  13. F. Belal, A. El-Brashy, N. El-Enany and N. El-Bahay, J. AOAC Int., 91, 1309 (2008).
  14. M. Walash, F. Belal, M. Eid and S.A. Abass, J. Fluoresc., 21, 555 (2011); doi:10.1007/s10895-010-0742-x.
  15. A.F.M. El Walily, S.F. Belal and R.S. Bakry, J. Pharm. Biomed. Anal., 14, 561 (1996); doi:10.1016/0731-7085(95)01662-7.
  16. K. Kelani, L.I. Bebawy and L. Abdel-Fattah, J. Pharm. Biomed. Anal., 18, 985 (1999); doi:10.1016/S0731-7085(98)00107-1.
  17. J. Rose, “Advanced Physico-Chemical Experiments”, Pitman, London; 1964. 54 p.
  18. P. Job, “Spectrochemical Methods of Analysis”, Wiley Interscience, New York; 1971. 346 p.
  19. J.E. Conte Jr., G. Wang, E.T. Lin and E. Zurlinden, J. Chromatogr. B Biomed. Sci. Appl., 753, 343 (2001); doi:10.1016/S0378-4347(00)00572-7.
  20. ICH, Q 2(R1); Validation of Analytical Procedures: Text and Methodology, International Conference on Harmonization. Geneva; 2005.
  21. J.N. Miller and J.C. Miller, “Statistics and Chemometrics for Analytical Chemistry”. 5th Ed., Pearson Education Limited, Harlow; 2005.
  22. P. Gomes and M. Steppe, J. AOAC Int., 89, 1296 (2006).
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 2 Download : 0