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Vol. 26 No. 3 (2014): Vol 26 Issue 3

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Immobilization of Soybean Hulls Peroxidase on Activated Carbon

https://doi.org/10.14233/ajchem.2014.15475
Huaili Zheng
Key Laboratory of Three Gorges Reservoir Region's Eco-Environment Ministry of Education, Chongqing University, Chongqing 400045, P.R. China
Xiadong Jiang
Key Laboratory of Three Gorges Reservoir Region's Eco-Environment Ministry of Education, Chongqing University, Chongqing 400045, P.R. China

Corresponding Author(s) : Xiadong Jiang

zhl@cqu.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 3 (2014): Vol 26 Issue 3

Abstract

To lay a scientific foundation for the development of an immobilized enzyme-based process for oxidizing dissolved organics in aqueous solution, the immobilization process of soybean hulls peroxidase (SBP) on activated carbon was investigated. Soybean hulls peroxidase (SBP, EC 1.11.1.7) was extracted from soybean hulls in phosphate buffer solution (0.1 M, pH 6.0) and then purified. The molecular weight of the soybean hulls peroxidase is 37 kDa. The soybean hulls peroxidase was immobilized onto activated carbon, the immobilization of soybean hulls peroxidase at pH 5.0 and higher temperature (50 ºC) gave rise to the highest immobilization yield. Equilibrium adsorption models were applied to quantify the effectiveness of immobilization. Adsorption of soybean hulls peroxidase on activated carbon followed the Langmuir model better than the Freundlich model. Fourier transform infrared spectra (FT-IR) provide evidence of the soybean hulls peroxidase enzyme immobilized onto the carbon matrices. The morphology of the native activated carbon and the immobilized soybean hulls peroxidase was confirmed by scanning electron microscopy (SEM).

Keywords

Soybean hulls peroxidase Immobilization Activated carbon
Zheng, H., & Jiang, X. (2014). Immobilization of Soybean Hulls Peroxidase on Activated Carbon. Asian Journal of Chemistry, 26(3), 685–689. https://doi.org/10.14233/ajchem.2014.15475
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