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Determination of Lawsone by HPTLC in Lawsonia inermis L. Callus and Plantlets Regenerated in vitro
Corresponding Author(s) : Moitreyee Saha
Asian Journal of Chemistry,
Vol. 26 No. 3 (2014): Vol 26 Issue 3
Abstract
Leaves of Lawsonia inermis L. (henna) contain lawsone (2-hydroxy-1, 4-naphthaquinone). The orange red dye is used for colouring hair and hands. Lawsone also exhibits antifungal, antibacterial and antioxidant activity. HPTLC technique was used for estimation of lawsone in Lawsonia inermis L. plantlets regenerated in vitro and callus derived from leaf explants of in vitro seedlings. The content of lawsone found was 0.057 μg/mg in plantlet regenerated in vitro and 0.017 μg/mg (callus powder) of Lawsonia inermis L.
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References
G. Chaudhary, S. Goyal and P. Poonia, Int. J. Pharm. Sci. Drug Res., 2, 91 (2010).
A.K. Gupta, Quality standards of Indian Medicinal Plants, Indian Council Medicinal Research, New Delhi, edn 1, Vol. 1p. 23 (2003).
M.G. Chauhan and A.P.G. Pillai, Microscopic profile of powdered drug used in Indian system of medicine, Gujarat Ayurved University, Jamnagar, Gujarat, edn 1, Vol. 2, pp. 84-85 (2007).
P.D. Sethi, Quantitative Analysis of Drugs in Pharmaceutical Formulations, CBS Publisher and Distributors, pp. 589 (1997).
T. Murashige and F. Skoog, Physiol. Plant, 15, 473 (1962); doi:10.1111/j.1399-3054.1962.tb08052.x
A.T. Bakkali, M. Jaziri, A. Foriers, Y. Vander Heyden, M. Vanhaelen and J. Homès, Plant Cell Tissue Organ Cult., 51, 83 (1997); doi:10.1023/A:1005926228301.
S.S. Phirke, M. Saha and N. Chandra, Asian J. Exp. Biol. Sci., 118 (2010).