Simultaneous Determination of Multi Components in Weitongding Particle by HPLC

Lin Tang; Shan Zhong

doi:10.14233/ajchem.2014.15523

Issue

Vol. 26 No. 3 (2014): Vol 26 Issue 3

Issue Published : January 30, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Simultaneous Determination of Multi Components in Weitongding Particle by HPLC

https://doi.org/10.14233/ajchem.2014.15523

Lin Tang

State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University (Guangzhou Medical University), No. 151 YanJiang Road, Yuexiu Dist. 510120, Guangzhou Guangdong Prov., P.R. China

Shan Zhong

School of Pharmaceutical Science, Sun Yat-sen University, No. 132 East Outer Ring Road, Guangzhou University City, 510006, Guangzhou Guangdong Prov., P.R. China

Asian Journal of Chemistry, Vol. 26 No. 3 (2014): Vol 26 Issue 3
Article Published : January 30, 2014

Abstract

An HPLC method for the simultaneous determination of multi components in Weitongding particle is established. HPLC conditions included Agilent Eclipse XDB-C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase was a mixture of buffer solution (A, containing 0.1 % formic acid) and methanol (B) at a flow rate of 1 mL min^-1 for the gradient elution and the column temperature was maintained at 35 °C. Five active ingredients had good linear relationships: astragalin in the range of 0.0036-0.1800 μg (R² = 0.9991), quercetin in the range of 0.0045-0.2250 μg (R² = 0.9992), isorhamnetin in the range of 0.0113-0.5650 μg (R² = 0.9990), a-tractlone in the range of 0.0108-0.5400 μg (R² = 0.9993) and hyperoside in the range of 0.0165-0.8250 μg (R² = 0.9992). Their average recoveries were 95.1 % (RSD = 2.4 %), 93.5 % (RSD = 2.2 %), 96.2 % (RSD = 2.2 %), 94.9 % (RSD = 1.9 %) and 97.6 % (RSD = 3.0 %), respectively. This method was steady with high precision and good repeatability and could be used for the determination of the five active ingredients and quality control of Weitongding particle.

Keywords

Weitongding particle HPLC Astragalin Quercetin Isorhamnetin a-Tractlone Hyperoside

Tang, L., & Zhong, S. (2014). Simultaneous Determination of Multi Components in Weitongding Particle by HPLC. Asian Journal of Chemistry, 26(3), 758–760. https://doi.org/10.14233/ajchem.2014.15523

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References

Y.F. Yang, Q.M. Wang, W.M. Zhang et al., J. Appetite Eastern Western Com., 4, 221 (1996).

F.H. Mu, F.L. Hu and G.L. Yang, J. World Digestion, 13, 72 (2007).

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L.L. Zhang, H.X. Chen and R.Q. Zhang, Chin. Communi. Doc., 31, 194 (2012).

Y.J. Wang, G.F. He, L.H. Yang, et al, J. Appetite Eastern Western Com., 3, 185 (1995).

K. Wada, M. Nihira, H. Hayakawa, Y. Tomita, M. Hayashida and Y. Ohno, Forensic Sci. Int., 148, 21 (2005); doi:10.1016/j.forsciint.2004.04.016.

E.M. Thurman, I. Ferrer, J.A. Zweigenbaum, J.F. García-Reyes, M. Woodman and A.R. Fernández-Alba, J. Chromatogr. A, 1082, 71 (2005); doi:10.1016/j.chroma.2005.03.042.

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Issue

Vol. 26 No. 3 (2014): Vol 26 Issue 3

Simultaneous Determination of Multi Components in Weitongding Particle by HPLC

Abstract

Keywords

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